SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION

Emine İrdem; Semra İlhan; Ercan Özbiçen; Lira Usakbek Kyzy; Gamze Tunca; Esma Ocak; Niyazi Can Zorluer; Uğur Çiğdem; Fatma Ayva; Rasime Demirel

doi:10.23902/trkjnat.1190972

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Year 2023, , 51 - 62, 15.04.2023

Emine İrdem , Semra İlhan , Ercan Özbiçen , Lira Usakbek Kyzy , Gamze Tunca , Esma Ocak , Niyazi Can Zorluer , Uğur Çiğdem , Fatma Ayva , Rasime Demirel

https://doi.org/10.23902/trkjnat.1190972

Abstract

Haloalkalitolerant funguslar, tuz konsantrasyonunun ve pH değerinin yüksek olduğu ortamlarda yaşayabilirler. Stresli koşullar altında üretilen biyoaktif bileşiklerin potansiyel biyoteknolojik uygulamaları vardır. Bu çalışmada, poliekstrem koşullara sahip Acıgöl/Türkiye'den izole edilen 52 mikrofungus, bazı biyoteknolojik özellikler açısından taranmıştır. Antimikrobiyal ve antioksidan aktiviteler sırasıyla agar difüzyon ve 2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürücü etki yöntemi kullanılarak belirlenmiştir. Enzim aktiviteleri, agar difüzyon tekniğinin kullanıldığı çeşitli yöntemlerle belirlenmiştir. Gümüş nanopartikül (AgNP) sentezi, hücre içermeyen mikrofungus filtratı kullanılarak gerçekleştirilmiştir. İzolatların %40'ı test mikroorganizmalarından en az birine karşı antimikrobiyal aktivite göstermiştir. Penicillium dipodomyicola, E. coli ve S. aureus’a karşı en yüksek antibakteriyel aktiviteyi gösterirken, P. brevicompactum Candida albicans'a karşı en yüksek aktiviteyi göstermiştir. Penicillium dipodomyicola ve P. bilaiae'nin pozitif kontrol ile rekabet edebilecek düzeyde (%90 ve üzeri) serbest radikal süpürücü aktiviteye sahip olduğu belirlenmiştir. Amilaz aktivitesine sahip izolatların tamamı Aspergillus ve Penicillium cinslerine ait olup, A. ochraceous, A. flavus ve P. brevicompactum en yüksek aktiviteye sahip türler olarak belirlenmiştir. İzolatların %55'i proteolitik aktivite gösterirken, A. alliaceus en yüksek aktiviteye sahip izolat olmuştur. Hemen hemen tüm izolatlar (%92) lipolitik aktivite göstermiştir. Özellikle A. amstelodami, P. sizovae ve P. solitum izolatlarının önemli düzeyde lipolitik aktiviteye sahip olduğu belirlenmiştir. İzolatların %35'i selülolitik aktivite göstermiş ve Cladosporium pseudocladosporioides, P. dipodomyicola ve P. bilaiae izolatlarında yüksek aktivite gözlenmiştir. İzolatların sekizi 24 saat içinde AgNP sentezini gerçekleştirmiştir. Tüm sonuçlar değerlendirildiğinde Aspergillus amstelodami, A. ochraceus, Penicillium dipodomyicola ve P. brevicompactum gibi izolatların farklı endüstriyel alanlarda hizmet verme potansiyeline sahip olduğu belirlenmiştir.

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SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION

Year 2023, , 51 - 62, 15.04.2023

Emine İrdem , Semra İlhan , Ercan Özbiçen , Lira Usakbek Kyzy , Gamze Tunca , Esma Ocak , Niyazi Can Zorluer , Uğur Çiğdem , Fatma Ayva , Rasime Demirel

https://doi.org/10.23902/trkjnat.1190972

Abstract

Haloalkalitolerant fungi can survive in environments with high salt concentrations and pH values. The bioactive compounds produced under stressful conditions have potential biotechnological applications. In this study, 52 microfungi isolated from Acıgöl Lake in Türkiye, offering polyextreme conditions, were screened for some biotechnological properties. The antimicrobial and antioxidant activities of the isolates were determined using the agar diffusion and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging methods, respectively. Enzyme activities were determined by various methods using the agar diffusion technique. Synthesis of silver nanoparticles (AgNPs) was carried out using cell-free filtrate of microfungi. 40% of the isolates showed antimicrobial activity against at least one of Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213 and Candida albicans ATCC 90028 used as the test microorganisms. Penicillium dipodomyicola showed the highest antibacterial activity against E. coli and S. aureus, while P. brevicompactum showed the highest activity against C. albicans. Penicillium dipodomyicola and P. bilaiae were found to have free radical scavenging activity of a level (90% and above) that can compete with positive control. All of the isolates with amylase activity belonged to Aspergillus and Penicillium and the most prominent three of them were A. ochraceous, A. flavus and P. brevicompactum. 55% of the isolates showed proteolytic activity, among which A. alliaceus had the highest activity. Almost all isolates (92%) showed lipolytic activity. Aspergillus amstelodami, P. sizovae and P. solitum had a significant level of lipolytic activity. 35% of the isolates showed cellulolytic activity with highest values Cladosporium pseudocladosporioides, P. dipodomyicola and P. bilaiae. Eight of the isolates carried out AgNP synthesis within 24 h. When all the results were evaluated, Aspergillus amstelodami, A. ochraceus, Penicillium dipodomyicola, and P. brevicompactum appeared to have the potential to serve in different industrial areas.

Keywords

polyextremophiles, alkaline lake, biological activity, microfungi

References

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4. Attia, E.Z., Farouk, H.M., Abdelmohsen, U.R. & Mo'men, H. 2020. Antimicrobial and extracellular oxidative enzyme activities of endophytic fungi isolated from alfalfa (Medicago sativa) assisted by metabolic profiling. South African Journal of Botany, 134: 156-162.
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6. Bodade, R. & Lonkar, K. 2022. Extremophilic Fungal Amylases: Screening, Purification, Assay, and Applications, pp. 465-487. In: Sahay, S. (ed.). Extremophilic Fungi, Springer, Singapore, 748 pp.
7. Bunbamrung, N., Intaraudom, C., Dramae, A., Komwijit, S., Laorob, T., Khamsaeng, S. & Pittayakhajonwut, P. 2020. Antimicrobial, antimalarial and anticholinesterase substances from the marine-derived fungus Aspergillus terreus BCC51799. Tetrahedron, 76(41): 131496. https://doi.org/10.1016/j.tet.2020.131496
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Details

Primary Language	English
Subjects	Microbiology
Journal Section	Research Article/Araştırma Makalesi
Authors	Emine İrdem 0000-0002-2955-298X Semra İlhan 0000-0002-3787-2449 Ercan Özbiçen 0000-0001-5646-6115 Lira Usakbek Kyzy 0000-0002-9424-4473 Gamze Tunca 0000-0002-4126-8340 Esma Ocak 0000-0002-9085-4151 Niyazi Can Zorluer 0000-0002-2394-2194 Uğur Çiğdem 0000-0003-4790-494X Fatma Ayva 0000-0002-7072-2928 Rasime Demirel 0000-0001-6702-489X
Publication Date	April 15, 2023
Submission Date	October 18, 2022
Acceptance Date	March 13, 2023
Published in Issue	Year 2023

Cite

APA	İrdem, E., İlhan, S., Özbiçen, E., Usakbek Kyzy, L., et al. (2023). SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. Trakya University Journal of Natural Sciences, 24(1), 51-62. https://doi.org/10.23902/trkjnat.1190972
AMA	İrdem E, İlhan S, Özbiçen E, Usakbek Kyzy L, Tunca G, Ocak E, Zorluer NC, Çiğdem U, Ayva F, Demirel R. SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. Trakya Univ J Nat Sci. April 2023;24(1):51-62. doi:10.23902/trkjnat.1190972
Chicago	İrdem, Emine, Semra İlhan, Ercan Özbiçen, Lira Usakbek Kyzy, Gamze Tunca, Esma Ocak, Niyazi Can Zorluer, Uğur Çiğdem, Fatma Ayva, and Rasime Demirel. “SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION”. Trakya University Journal of Natural Sciences 24, no. 1 (April 2023): 51-62. https://doi.org/10.23902/trkjnat.1190972.
EndNote	İrdem E, İlhan S, Özbiçen E, Usakbek Kyzy L, Tunca G, Ocak E, Zorluer NC, Çiğdem U, Ayva F, Demirel R (April 1, 2023) SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. Trakya University Journal of Natural Sciences 24 1 51–62.
IEEE	E. İrdem, “SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION”, Trakya Univ J Nat Sci, vol. 24, no. 1, pp. 51–62, 2023, doi: 10.23902/trkjnat.1190972.
ISNAD	İrdem, Emine et al. “SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION”. Trakya University Journal of Natural Sciences 24/1 (April 2023), 51-62. https://doi.org/10.23902/trkjnat.1190972.
JAMA	İrdem E, İlhan S, Özbiçen E, Usakbek Kyzy L, Tunca G, Ocak E, Zorluer NC, Çiğdem U, Ayva F, Demirel R. SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. Trakya Univ J Nat Sci. 2023;24:51–62.
MLA	İrdem, Emine et al. “SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION”. Trakya University Journal of Natural Sciences, vol. 24, no. 1, 2023, pp. 51-62, doi:10.23902/trkjnat.1190972.
Vancouver	İrdem E, İlhan S, Özbiçen E, Usakbek Kyzy L, Tunca G, Ocak E, Zorluer NC, Çiğdem U, Ayva F, Demirel R. SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION. Trakya Univ J Nat Sci. 2023;24(1):51-62.

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