EVALUATION OF QUORUM SENSING SIGNALS OF STRONG BIOFILM PRODUCING BACTERIA VIA LC-MSMS, HPLC AND BIOSENSORS

Year 2023, , 23 - 30, 15.10.2023

Dilvin İpek

https://doi.org/10.23902/trkjnat.1276562

Abstract

This study aimed to show presence of Quorum Sensing (QS) signals of Gram-negative and Gram-positive biofilm producing bacteria isolated from real dairy process lines. Defining the profile and chemical composition of QS-signals is an important factor in control of microbial resistance and biofilm production. We especially focused on unusual behaviour of Gram-positive and Gram-negative isolates. Long-chain acyl-homoserine lactones (AHLs) signals (C14-HSL, C16-HSL and C18-HSL) and DFD (4,5-dihidroksi-2,3-pentanedione)-AI-2 signals of the isolates were studied by High-performance liquid chromatography (HPLC) and Liquid Chromatography with tandem mass spectrometry (LC-MSMS) methods. All Gram-positive isolates were defined as AHL-producers. All Gram-negative isolates, formerly defined as non-AHL producers by both biosensors and HPLC methods, were identified as AHL-producers. DFD signal was only detected from Gram-negative Klebsiella pneumonia, Enterobacter cloacae and Klebsiella oxytoca isolates. The results demonstrated that the QS-system is a complex system and biosensor microorganism may not be the best method for QS-signal identification. The results also provided new insights in defining the profile and chemical composition of QS-signals importance for interrupting the chemical communication completely to reduce biofilm formation and prevent resistance gain of microorganisms.

Keywords

AHL Signals, AI-2 Signals, Biofilm, Biosensors, HPLC, LC-MSMS

Supporting Institution

Scientific Research Project Commission of Çanakkale Onsekiz Mart University

Project Number

FBA-2018-1438

Thanks

I am thankful to Assoc.Prof.Dr. Nükhet N. Demirel Zorba’s laboratory members for their kind support, Food Engineering Department, Çanakkale Onsekiz Mart University, Çanakkale, Turkey. I am also thankful to Çanakkale Onsekiz Mart University Science and Technology Application and Research Center, Çanakkale Onsekiz Mart University, Çanakkale, Turkey for their cooperation in LC-MSMS study. I express my sincere thanks to Prof.Dr. Ji Hyang Kweon and Assist. Prof.Dr. Harshade Lade and their research team, Water Treatment and Membrane Laboratory, Department of Environmental Engineering, Konkuk University, Seoul, Korea for their kind cooperation in HPLC study.

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