FcMgv1, FcStuA AND FcVeA BASED GENETIC CHARACTERIZATION IN Fusarium culmorum (W.G. Smith)

Year 2018, , 63 - 69, 24.03.2018

Emre Yörük , Özlem Sefer

https://doi.org/10.23902/trkjnat.334792

Abstract

Dünya
çapında pek çok ülkede olduğu gibi Türkiye’de de Fusarium culmorum (W.G. Smith) başak yanıklığı ve kök çürüklüğü
hastalıkları ile arpa ve buğday tarım alanlarında ekonomik kayıplara yol açar.
Bu çalışmada, Türkiye’den köken alan 33 F.
culmorum izolatının in vitro
büyüme kapasitesi ile FcMgv1, FcStuA ve FcVeA genetik benzerliği aracılığı ile elde edilen fenotipik ve
genetik karakterlerin ilişkisi incelenmiştir. Doğrusal büyüme oranı değerleri
inkübasyonun 4. ve 7. günlerinde kaydedilmiştir. Ortalama doğrusal büyüme
oranlarının 7,58±1.06 ve 14,7±1.26mm/gün arasında olduğu görülmüştür. Göreceli
olarak yüksek LGR değerlerine sahip olduğu belirlenen F2 ile 18F izolatları ile
göreceli olarak düşük LGR değerlerine sahip olduğu belirlenen 12F ve F19
izolatları multilokus temelli genotiplendirme analizlerinde kullanılmak üzere
seçilmiştir. FcMgv1, FcStuA ve FcVeA genlerine ait
sırasıyla 1733, 2001 ve 1898bç ürünler elde edilmiştir. Genler dizilenmiş, bir
araya getirilmiş ve BLASTn ile maksimum olasılık topoloji analizi yapılmıştır.
Her bir genin nükleotid dizisi NCBI'da 0,0-0,0 E-değeri ve 1188-3256 arası bit
skoru vermiştir. Hizalama analizi en az %89 ön yükleme değeri ile
sonuçlanmıştır. Ayrıca, benzer büyüme oranına sahip izolatlar filogenetik
analizlerde aynı alt kümede yer almıştır. Bu çalışmada elde edilen bulgular,
fungal yaşam için gerekli olan bu üç genin, genetik karakterizasyonda ve
fenotipik ve genotipik özellikleri arasında ilişki kurulmasında
kullanılabileceğini ortaya konmuştur. 

Keywords

Fusarium culmorum, PCR, genetic characterization, genetic diversity, linear growth rate

FcMgv1, FcStuA AND FcVeA BASED GENETIC CHARACTERIZATION IN Fusarium culmorum (W.G. Smith)

Abstract

Fusarium culmorum (W.G. Smith) leads
to economic losses in wheat and
barley fields in Turkey as well as in many countries worldwide as a result of
head blight and crown rot diseases. In this study, in vitro growth capacity of 33 F.
culmorum isolates originating from Turkey and the relationship between phenotypic
and genetic characteristics obtained based on similarities of FcMgv1, FcStuA and FcVeA genes
were investigated. Linear growth rate
values were recorded at 4^th and 7^th days of incubation.
The mean linear growth rate values ranged from 7.58±1.06
to 14.7±1.26mm/day. The isolates F2 and 18F with relatively high linear
growth values and the isolates 12F and F19 with relatively low linear growth
values, were selected to be used in multiloci based genotyping analysis. FcMgv1, FcStuA and FcVeA genes
were amplified in lengths of 1733, 2001 and 1898bp, respectively. The genes
were sequenced, aligned and then subjected to BLASTn and to maximum likelihood
topology analysis. Nucleotide sequence of each gene showed maximum hit with
associated genes deposited in NCBI with 0.0-0.0 E-values and 1188 to 3256 bit
scores. Alignment analysis resulted in at least 89% bootstrap support.
Moreover, isolates with similar linear growth rates were co-clustered in
phylogenetic analysis. The findings obtained in this study showed that the
three genes which are essential for fungal survival could be used in genetic
characterization analysis and in revealing the associations between their
genetic and phenotypic characteristics.

Keywords

Fusarium culmorum, PCR, genetic characterization, genetic diversity, linear growth rate

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