INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY

Year 2018, , 147 - 157, 15.10.2018

Pelin Turhan Serttaş , Tamer Özcan

https://doi.org/10.23902/trkjnat.433329

Cited By: 3

Abstract

In this study, intra-specific variations in
naturally growing and cultivated mastic tree (Pistacia lentiscus L.) samples obtained from western parts of
Turkey were examined using ISSR and IRAP marker techniques. Samples from Crete
and Chios were also included in the study. Morphological measurements of some
leaf characteristics of the samples were performed and the measured data was evaluated
statistically with a Pearson Correlation analysis to reveal the correlations
between character pairs. ISSR primers produced 81 bands between 161-1884bp with
96.3% polymorphism and IRAP primers produced 72 bands between
124-2027bp with 91.67% polymorphism. Polymorphism information content (PIC)
values were 0.458 and 0.418 for ISSR and IRAP, respectively. Genetic similarity
matrix was examined with Jaccard’s coefficient. Maximum similarity was found
between the Cretan samples (LG2 and LG3) with the ISSR analysis (0.933) and
between L25A (C1, Bodrum) and L29A (C1, Milas) with the IRAP analysis (0.593).
Unweighted pair group method with arithmetic mean (UPGMA) dendrogram was
divided into 12 and 4 groups by ISSR and IRAP methods, respectively. Specimens
were segregated on 3 main different clusters by the Principal Component
Analysis (PCA) based on the combined marker systems. The results showed that P. lentiscus has very high ratios of
intraspecific variation. The present work is an original study in terms of
large sampling including wild genotypes, cultivated specimen, Chios and Cretan
varieties, use of ISSR and IRAP combination, determination of relations between
culture and wild genotypes and the use of Bagy-1
retrotransposons in intraspecific polymorphism. This study may be considered as
a reference study for studies on gene pools of P. lentiscus and phylogenetic relationships within the species and
may contribute to species concept and agricultural breeding programs.

Keywords

IRAP, ISSR, Phylogenetics analysis

INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY

Abstract

Bu
çalışmada, ISSR ve IRAP markör teknikleri kullanılarak Türkiye’nin batı
kesiminde doğal olarak yetişen ve kültürü yapılan sakız ağacı (Pistacia lentiscus L.) örneklerindeki
tür içi varyasyon analizi yapılmıştır. Girit ve Sakız Adası örnekleri de
çalışmaya dahil edilmiştir. Bazı yaprak özelliklerinin morfolojik ölçümleri
gerçekleştirilmiş ve aralarındaki korelasyon Pearson Korelasyon analizi ile
belirlenmiştir. ISSR primerleri 161-1884bç arasında %96,3 polimorfizm ile 81
bant üretmiştir. IRAP primerleri ise 124-2027bç arasında %91,67 polimorfizm ile
72 bant üretmiştir. Polimorfizm bilgi içeriği (PIC) değerleri 0,458 (ISSR) ve
0,418 (IRAP) arasında bulunmuştur. Genetik benzerlik matriksleri Jaccard
katsayısıyla oluşturulmuştur. ISSR sonuçlarında en yüksek benzerlik Girit
örnekleri (LG2 ve LG3) arasında (0,933) ve IRAP sonuçlarında ise L25A (C1,
Bodrum) ve L29A (C1, Milas) arasında bulunmuştur. UPGMA yöntemiyle kurulan
dendrogramlarda sırasıyla ISSR için 12 grup ve IRAP için 4 grup ayrılmıştır.
Her iki markör sistemi için ortak kurulan temel bileşen analizi (PCA)
grafiğinde 3 farklı küme oluşmuştur. Sonuçlar göstermiştir ki, P. lentiscus yüksek oranlarda tür içi
varyasyona sahiptir. Bu çalışma, ISSR ve IRAP markörlerinin yabani genotipler,
kültür türleri, Sakız Adası ve Girit çeşitlerinin analizinde kullanımı, kültür
varyeteleri ve yabani genotipler arasındaki ilişkilerin belirlenmesi ve tür içi
polimorfizmde Bagy-1
retrotranspozonlarının kullanımı açısından özgün bir çalışmadır. Söz konusu
çalışma, P. lentiscus gen havuzunun
ve filogenetik ilişkilerinin araştırılması, tür sınırlarını saptamaya yönelik
yapılacak tarımsal ıslah çalışmaları için referans niteliğindedir.

Keywords

IRAP, ISSR, Phylogenetics analysis

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