FITNESS AND PATHOGENICITY OF OUTBREAK CAUSING Salmonella UPON SHORT-TERM EXPOSURE TO GROUNDWATER WITH RESIDUAL ANTIBIOTICS

Year 2019, , 1 - 8, 15.04.2019

Berat Zeki Haznedaroğlu

https://doi.org/10.23902/trkjnat.435384

Abstract

The changes in survival and
pathogenicity of three Salmonella
enterica subsp. enterica
serotypes upon short term exposure to groundwater with residual antibiotics
have been studied in relationship to overall microbial fitness. A wild type
flagellated Salmonella enterica ser.
Typhimurium outbreak strain, a mutant Salmonella
enterica ser. Typhimurium strain, and a wild type avian disease-causing Salmonella enterica ser. Pullorum strain
were exposed to a range of ionic strength (3-30 mM) groundwater with residual
antibiotics for 6-24 hours. Exposed organisms’ pathogenicity was tested in
vitro exposure to a human epithelial cell line (HEp2). Resistance profiles
against 10 common antibiotics were also tested and compared to unexposed
controls. Results show minor antibiotic resistance changes for S. enterica ser. Typhimurium strains in
response to some antibiotic classes mediated with active efflux pumps. This
trend was not observed for S. enterica ser.
Pullorum, suggesting that resistance found in groundwater exposed organisms
might be strain-dependent. In vitro epithelial cell invasion assays showed
bacterial invasion of HEp2 cells initially decreases with time and increases
after 24 hours. It is concluded that S.
enterica serotypes reaching groundwater environments in the presence of
residual antibiotics may exhibit increased levels of pathogenicity,
strain-dependent resistance to antibiotics, and sustained levels of viability.

Keywords

Salmonella, antibiotic resistance, invasion, groundwater, pathogenicity

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