BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE

Year 2019, , 27 - 34, 15.04.2019

Müslüm Altun

https://doi.org/10.23902/trkjnat.448851

Cited By: 4

Abstract

Polyglutamic acid (PGA) is water-soluble and biodegradable polymer with
high production cost. For feasible PGA production, feather hydrolysate (FH) was
used as fermentation substrate. 30L fermentation of native feather was realized
to obtain keratinase enzyme using Streptomyces
pactum DSM 40530. Fermentation broth was concentrated by cross-flow
filtration where the enzyme activity increased by 8.75-fold and 8×10³UL^-1d^-1
of enzyme activity was the optimum for achieving 75% degradation per gram of
feather. 40g/L of FH was used with different media compositions to produce PGA
using Bacillus licheniformis 9945a.
Among four different cultivation where L-glutamate, tri-sodium citrate and
glycerol were used as the constituents of Medium E, highest yields of γ-PGA and
cell dry matter (CDM) were obtained from cultivation-1, at 5.4±0.4 and
8.6±0.5g/L, respectively, despite the culture media did not contain glutamic
acid. In cultivation-2, which was not only missing glutamate but also citrate,
the γ-PGA and CDM yielded 3.2±0.2 and 7.8±0.4g/L, respectively whereas it was
only 1.9±0.2 and 4.2+0.4g/L when FH was used as the sole substrate in
cultivation-3. When cultivation-4 was adopted where only glycerol was missing,
the γ-PGA and CDM yields slightly increased to 2.3±0.2 and 5.5±0.3g/L,
respectively. This is the first study that achieved the production of γ-PGA
from FH.

Keywords

γ-Poly(glutamic acid), γ-PGA, feather hydrolysate, keratinolytic activity, feather

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