DETERMINATION OF in vitro BIOFILM FORMATION ABILITIES OF FOOD BORNE Salmonella enterica ISOLATES

Year 2019, , 57 - 62, 15.04.2019

Deniz Aksoy

https://doi.org/10.23902/trkjnat.471236

Cited By: 5

Abstract

Salmonellosis caused by non-typhodial Salmonella enterica serotypes is one of
the most important food-borne diseases worldwide and biofilm structure formed
by these pathogens provide a reservoir for food contamination and a source for
infections. This study was performed in order to determine biofilm formation abilities
of food borne Salmonella isolates on
polystyrene and on air liquid interphase and their colony morphologies when
grown on Congo Red Agar plates. 32 food-borne Salmonella strains isolated from retail chicken carcasses in Edirne
province of Turkey and belonging to the Infantis, Enteritidis, Kentucky and
Telaviv serotypes were used. The microtiter plate technique was used to
determine biofilm formation abilities of the isolates on polystyrene surfaces
by measuring the optical density (OD) values of the stained bacterial biofilms.
The results showed that the strongest biofilm formation capacities of the
isolates were observed at 22°C for 3 days of incubation. Although all isolates
formed pellicle on the liquid-air interface at 22°C, only 13% of the isolates
belonging to the Infantis, Kentucky and Enteritidis serovars formed pellicle at
liquid-air interface at 37°C. Three different colony morphotypes (saw; smooth
and white, bdar; brown, dry and rough, rdar; red, dry and rough) were
determined on Congo Red Agar among the isolates. High biofilm formation
abilities of the tested Salmonella
isolates can lead to widespread of virulence and resistance properties,
especially to medically important antibiotics such as ciprofloxacin, via food
chain. This situation constitutes an important concern for public health.

Keywords

Salmonella, biofilm, food-borne pathogen, microbial food safety

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