In vitro evaluation of the effects of potential GSK-3β inhibitors terbutaline and orciprenaline

Year 2024, Volume: 25 Issue: 1, 73 - 80, 15.04.2024

Ebru Uzunhisarcıklı

https://doi.org/10.23902/trkjnat.1356270

Abstract

Lung cancer is a type of cancer that is mostly diagnosed at an advanced stage and has a short survival time despite standard chemotherapy and targeted therapies. Terbutaline and Orci-prenaline are bronchodilator agents that are potent and selective β2 receptor agonists. The purpose of this study was to investigate to evaluate the effects of Terbutaline and Orci-prenaline on A549 human lung carcinoma cell line and Beas-2b human bronchial epithelial cell line. Cells were treated with 1, 10, 100, 200, 400 µM concentrations of Terbutaline and Orciprenaline. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay and xCELLigence real-time cell analyzer were used to determine their effects on cell viability. The cell index was monitored continuously by visualizing the impedance of the E-plate wells. Because of the roles of Glycogen Synthase Kinase 3β (GSK3β) in a diverse range of cellular processes like metabolism, cell proliferation, differentiation and survival and its key position at several signaling pathways, GSK3β inhibition by Terbutaline and Orciprenaline was also investigated. The results showed that Terbutaline and Orciprenaline inhibits GSK-3β. The overall results led to the conclusion that Terbutaline and especially Orciprenaline may have potential therapeutic effects in lung carcinoma.

Keywords

Lung cancer, Cytotoxicity, Bronchodilator agents, xCELLigence, Glycogen synthase kinase

Ethical Statement

Since the article does not contain any studies with human or animal subject, its approval to the ethics committee was not required.

Supporting Institution

Erciyes University Scientific Research Projects Coordination Unit

Project Number

TLO-2022-12397 and TLO-2022-12398.

Thanks

I would like to thank Rukiye Aslan (Kayseri, Türkiye) for her help in the experiments.

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