Determination of Acute Effects of 2,4-Dichlorophenoxyacetic Acid on Antioxidant Enzymes and Lipid Peroxidation Levels in Zebrafish (Danio rerio Hamilton, 1822) Gills

Year 2017, Volume: 18 Issue: 2, 143 - 148, 20.10.2017

Figen Esin Kayhan , Güllü Kaymak , Cansu Akbulut , Nazan Deniz Yön Ertuğ

https://doi.org/10.23902/trkjnat.294722

Cited By: 1

Abstract

The
aim of this study is to determine the potential of 2,4-D
(2,4-Dichlorophenoxyacetic acid) herbicide in causing acute oxidative stress in
the gills of zebrafish (Danio rerio
Hamilton, 1822) using spectrophotometric methods. Test animals except the
control group (Group-K) were exposed to sublethal doses (0.1ppm, 0.5ppm and
1ppm) of 2,4-D herbicide for 96 hours. Malondialdehyde (MDA) was used to
determine lipid peroxidation levels, and reduced glutathione (GSH), catalase
enzyme activity (CAT) and total protein (TP) levels were determined to
determine their effects on antioxidant systems in gill tissues. In this study,
total protein levels in gills of zebrafish exposed to sublethal doses of 2,4-D
were observed to be reduced compared to the control group. MDA levels
significantly increased compared to the control group. GSH levels increased in
the highest dose of 2,4-D herbicide but decreased in other doses. No
significant difference was found in CAT activity compared to the control group.

Keywords

2.4-Dichlorophenoxyacetic acid, Antioxidant enzymes, gill, zebrafish

2,4-D (DİKLOROFENOKSİASETİK ASİT)’İN ZEBRA BALIĞI (Danio rerio) SOLUNGAÇLARINDA ANTİOKSİDAN ENZİMLER VE LİPİD PEROKSİDASYON SEVİYESİ ÜZERİNE AKUT ETKİLERİNİN BELİRLENMESİ

Abstract

Bu
çalışmanın amacı; 2-4-D (2,4-Diklorofenoksiasetik asit) herbisitinin, zebra
balığı (Danio rerio Hamilton, 1822)
solungaçlarında akut oksidatif strese neden olma potansiyellerinin
spektrofotometrik yöntemlerle belirlenmesidir. Kontrol grubu (Grup-K) dışındaki
balıklar, 96 saat süreyle 2-4-D herbisitinin subletal dozlarının (0.1, 0.5 ve 1
ppm) etkisine bırakılmıştır. Solungaç dokusunda lipid peroksidasyon
seviyelerinin belirlenmesi amacıyla malondialdehit (MDA), antioksidan
sistemlere etkisini belirlemek amacıyla da indirgenmiş glutatyon (GSH), katalaz
enzim aktivitesi (CAT) ve total protein (TP) seviyeleri spektrofotometrik
yöntemler kullanılarak belirlenmiştir. Bu çalışmada 2,4-D’nin subletal
dozlarına maruz bırakılan zebra balıklarının solungaçlarında total protein seviyelerinin
kontrol grubuna oranla azaldığı gözlenmiştir. MDA seviyeleri kontrol grubuna
oranla önemli ölçüde artmıştır. GSH seviyeleri 2-4-D herbisitinin en yüksek
dozunda artarken diğer gruplarda azalmıştır. CAT aktivitesinde ise kontrol
grubuna oranla önemli bir fark gözlenmemiştir.

Keywords

antioksidan enzimler, solungaç, zebra balığı, 2-4Diklorofenoksiasetik asit

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