SCREENING FOR ANTI-QUORUM SENSING AND ANTI-BIOFILM ACTIVITY IN Viscum album L. EXTRACTS AND ITS BIOCHEMICAL COMPOSITION

Year 2018, Volume: 19 Issue: 2, 175 - 186, 15.10.2018

Demet Erdönmez , Nihal Kenar Kübra Erkan Türkmen

https://doi.org/10.23902/trkjnat.369911

Cited By: 6

Abstract

Fırsatçı
patojenik bakteriler, virülans ifadelerini koordine etmek için Quorum Sensing
(QS) sistemini kullanır. Dolayısıyla QS sistemi, bakteriyel enfeksiyonların
kontrolü için yeni bir yaklaşım olarak tercih edilebilir. Bu çalışmanın amacı,
2 Gram-pozitif, 7 Gram-negatif patojenik bakteri üzerinde analiz edilen Viscum album L. bitkisinin
gövde, yaprak ve meyve gibi bölümlerine ait etanol, kloroform ve
diklorometan:metanol ekstraktlarının antimikrobiyal, anti-biyofilm ve
anti-quorum sensing aktivitelerinin değerlendirilmesidir. Kullanılan bitki
parçası ekstraktına ve test mikroorganizmasına bağlı olarak, 50-250mg/ml
arasında değişen konsantrasyonlarda ekstraklar test edildi. 50mg/ml’lik konsantrasyonda antimikrobiyal
aktivite görülmediği için anti-quorum
sensing ve antibiyofilm çalışmalarında bu konsantrasyon kullanılmıştır. En iyi biyolojik aktivitenin
görüldüğü çözücünün ise diklorometan:metanol olduğu saptandı. Ekstraktların anti-quorum sensing aktiviteleri, Chromobacterium violaceum CV026
biyosensör suşu ve sinyal molekülü N- (β-ketokaproil) -L-homoserin lakton
(3-okso-C6-HSL)’nün bulunduğu besiyerinde agar difüzyon deneyi kullanılarak da
tespit edilmiştir. Biyofilm, mikrotiter plaka testi ve kristal viyole
kullanılarak ölçülmüştür. Yaprak
ve gövde kısımlarının antimikrobiyal, anti-biyofilm ve anti-quorum sensing
aktivitesi, meyve ekstraktına göre daha yüksek verimlilik göstermiştir. V.
album özütlerinin, biyofilm ya da QS inhibisyonu yoluyla
mikrobiyal enfeksiyonları tedavi etme potansiyeline sahip olduğu kanısına
varılmıştır.

Keywords

Anti-quorum sensing, Anti-biofilm activity, Antibacterial activity, Viscum album L.

SCREENING FOR ANTI-QUORUM SENSING AND ANTI-BIOFILM ACTIVITY IN Viscum album L. EXTRACTS AND ITS BIOCHEMICAL COMPOSITION

Abstract

Many opportunistic pathogenic bacteria use the Quorum Sensing (QS)
system to coordinate their virulence expressions. Thus, QS can likely be a new
approach to control bacterial infections. The aim of this study was to evaluate
the antimicrobial, anti-biofilm, and anti-quorum sensing activities of
ethanol, chloroform, and dichloromethane: methanol extracts of leaf, stem, and
fruits of the European mistletoe Viscum
album L. on 2 Gram-positive and 7 Gram-negative pathogenic bacteria. The extracts
at concentrations ranging from 50 to 250mg/ml were tested depending on the
extracts of the plant parts and the test bacteria. The extract with 50mg/ml
concentration, in which no antimicrobial activity was observed, was used for
anti-quorum sensing and antibiofilm studies. The dichloromethane: methanol
extracts were found to show the highest biological activities. QS activities of the plant
extracts were also determined using the recently established Chromobacterium
violaceum CV026 reporter strain and the signaling molecule N-(ß-ketocaproyl)-L-homoserine lactone
(3-oxo-C6-HSL) agar well diffusion assay. Biofilm was
quantified using the microtiter plate test and the crystal violet assay.
Anti-microbial, anti-biofilm, and anti-quorum sensing activity of leaf
and stem extracts showed higher efficiency than fruit extracts. It was concluded that
the extracts of V. album had the
potential to treat microbial infections by biofilm inhibition or inhibition of
QS.

Keywords

Anti-quorum sensing, Anti-biofilm activity, Antibacterial activity, Viscum album L.

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