OBTAINING CANDIDATE SALT TOLERANT WHEAT MUTANT LINES DERIVED FROM COMBINATION OF SODIUM AZIDE MUTAGENESIS AND SOMATIC EMBRYOGENESIS

Year 2019, Volume: 20 Issue: 2, 129 - 134, 15.10.2019

Ayşe Şen , Fatma Sarsu

https://doi.org/10.23902/trkjnat.571255

Cited By: 1

Abstract

Plant mutants are
important bio-resources for crop breeding and functional gene studies. In the
present study, conventional chemical mutagenesis technique was combined with
somatic embryogenesis to obtain candidate salt tolerant mutant wheat lines. For
this purpose, 0-5 mM Sodium Azide (NaN₃) was applied for 30 minutes
to embryonic calli under in vitro
conditions to produce genetic variations in the bread wheat (Triticum aestivum L. cv. Adana 99).
Treated and non-treated calli were put in somatic embryo induction media, and 3
and 4 mM NaN₃ were determined as optimum mutation doses for somatic
embryo induction. The obtained somatic embryos from these optimum mutagen doses were then
screened for tolerance in regeneration media containing 125 mM NaCl to be used
to improve tolerance to salt stress. In NaN₃ treatment, 14 mutants
with moderate salt tolerance were obtained. The results suggest that the in vitro technique in combination with
chemical mutagenesis may be a useful approach for accelerating breeding
strategies to create enough genetic variation in populations and to get fourth
generation putative salt tolerant wheat mutant lines it less than 1.5 years.

Keywords

Sodium azide, in vitro mutagenesis, salt tolerance, somatic embryogenesis, wheat

Supporting Institution

The Research Fund of Istanbul University

Project Number

BEK-2017-24939, 24750, 45260 and 49463

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