Research Article
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Year 2021, Volume: 22 Issue: 2, 139 - 146, 15.10.2021
https://doi.org/10.23902/trkjnat.873651

Abstract

Tuber P. Micheli ex F.H. Wigg. (trüf) türleri, güçlü aromaları, gastronomik ve ekonomik önemleri, yüksek çeşitlilikleri, bitki ekolojisi ve hayvan beslenmesinde olan önemleri ile her zaman insanların ilgisini çekmiştir. Türkiye'de trüf mantarı türlerine olan ilgi son yıllarda artmaktadır. Türkiye'de daha önce bazı trüf mantarı türleri bilinmesine rağmen, pek çoğu da henüz tanımlanmamıştır. Yeni trüf mantarı türlerinin bulunması amacıyla 2017 yılında Edirne ve Tekirdağ bölgelerinden Tuber macrosporum Vittad. örnekleri toplanmıştır. Tuber macrosporum örnekleri ilk olarak makro ve mikroskobik özellikleriyle tanımlanmış ve bu sonuç DNA analizleri ile GenBank'a göre % 99 olarak desteklenmiştir.
Yeni rapor edilen türün kısa bir tanımı, makro ve mikro fotoğrafları ve taramalı elektron mikroskobu (SEM) ile alınan spor görüntüleri ile birlikte verilmiştir. Ek olarak, türün ITS'e dayalı evrimsel geçmişi filogenetik ağaçla verilmiştir.

Project Number

BAP/20401081

References

  • 1. Akata, I., Sevindik, M. & Şahin, E. 2020. Tuber fulgens Quél. A New record for Turkish truffles. Turkish Journal of Agriculture-Food Science and Technology, 8(11): 2472-2475.
  • 2. Alkan, S., Aktaş, S. & Kaşık, G. 2018. Türkiye’deki Tuber türleri ve Tuber aestivum için yeni bir lokalite. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, 44(1): 25-29.
  • 3. Astier, J. 1998. Truffes Blanches et Noires (Tuberaceae & Terfeziaceae). Louis-Jean, 127 pp.
  • 4. Bencivenga, M. & Baciarelli Falini, L. 2012. Manuale di Tartuficoltura. Esperienze di Coltivazionedei Tartufi in Umbria. Assessorato Regionale Agricoltura e Foreste, Regione Umbria, 137 pp.
  • 5. Benucci, G.M.N., Goga Csorbai, A., Di Massimo, G., Baciarelli Falini, L., Bencivenga, M. & Donini, D. 2012. Mycorrhization of Quercus robur L. and Corylus avellana L. seedlings with Tuber macrosporum Vittad. Mycorrhiza, 22(8): 639-646.
  • 6. Breitenbach, J. & Kränzlin, F. 1983. Fungi of Switzerland: Vol.1. Ascomycetes. Verlag Mykologia, Luzern, 310 pp.
  • 7. Castellano, M.A. & Türkoğlu, A. 2012. New records of truffle taxa in Tuber and Terfezia from Turkey. Turkish Journal of Botany, 36(3): 295-298.
  • 8. Elliott, T.F., Türkoğlu, A., Trappe, J.M. & Yaratanakul, G.M. 2016. Turkish truffles 2: eight new records from Anatolia. Mycotaxon, 131: 439-453.
  • 9. Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39: 783-791.
  • 10. Gezer, K., Kaygusuz, O., Çelik, A. & Işıloğlu, M. 2014. Ecological characteristics of truffles growing in Denizli Province, Turkey. Journal of Food, Agriculture & Environment, 12(2): 1105-1109.
  • 11. Gori, L. 2005. Funghi Ipogei Della Lucchesia di Aitre Province Italiane e dall' Estero. Pacini Fazzi, 316 pp.
  • 12. Granetti, B., De Angelis, A. & Materozzi, G. 2005. Umbria, Terra di Tartufi. Regione Umbria-Gruppo Micologico Ternano, Terni, 303 pp.
  • 13. Hall, I., Brown, G. & Zambonelli, A. 2007. Taming the Truffle. The history, Lore, and Science of the Ultimate Mushroom. Timber Press, Oregon, 304 pp.
  • 14. Kaya, A. 2009. Macromycetes of Kahramanmaraş province (Turkey). Mycotaxon, 108: 31-34.
  • 15. Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 111-120.
  • 16. Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evoluation, 35: 1547-1549.
  • 17. Ławrynowicz, M., Krzyszczyk, T. & Fałdziński, M. 2008. Occurrence of black truffles in Poland. Acta Mycologica, 43(2): 143-151.
  • 18. Marjanović, Ž., Grebenc, T., Marković, M., Glišić, A. & Milenković, M. 2010. Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula. Sydowia, 62(1): 67-87.
  • 19. Medardi, G. 2006. Ascomiceti d’Italia Atlante Fotografico. Fondazione Centro Studi Micologici Dell’A.M.B., Vicenza, 678 pp.
  • 20. Medardi, G. 2012. Atlante Fotografico Degli Ascomiceti d’Italia. Fondazione Centro Studi Micologici Dell’A.M.B. Vicenza, 454 pp.
  • 21. Montecchi, A. & Sarasini, M. 2000. Funghi Ipogei d'Europa. Associazione Micologica Bresadola, Trento, 714 pp.
  • 22. Öztürk, C., Kaşık, G. & Toprak, E. 1997. Ascomycetes makrofunguslarından Türkiye için iki yeni kayıt. Ot Sistematik Botanik Dergisi, 4: 53-56.
  • 23. Pegler, D.N., Spooner, B.M. & Young, T.W.K. 1993. British Truffles: A revision of British Hypogeous fungi. The Royal Botanic Gardens, Kew, 216 pp.
  • 24. Sesli, E., Asan, A., Selçuk, F. (eds.), Abacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H.H., Erdoğdu, M., Ergül, C.C., Eroğlu, G., Giray, G., Haliki Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Ya., Uzun, Yu. & Yoltaş, A. (2020). Türkiye Mantarları Listesi. Ali Nihat Gökyiğit Vakfı Yayını. İstanbul, 1192 pp.
  • 25. Şen, I., Allı, H. & Civelek, H.S. 2016. Checklist of Turkish truffles. Turkish Journal of Life Sciences, 1(2): 103-109.
  • 26. Stobbe, U., Büntgen, U., Sproll, L., Tegel, W. & Egli, S. 2012. Spatial distribution and ecological variation of re-discovered German truffle habitats. Fungal Ecology, 5(5): 591-599.
  • 27. Thompson, P.I. 2013. Ascomycetes in Colour: Found and Photographed in Mainland Britain. Xlibris, Lexington KY, 408 pp.
  • 28. Trappe, M., Evans, F. & Trappe, J. 2007. Field Guide to North American Truffles: Hunting, Identifying, and Enjoying the World's Most Prized Fungi. Ten Speed Press, Berkeley, Toronto, 136 pp.
  • 29. Trappe, J.M., Molina, R., Luoma, D.L., Cázares, E., Pilz, D., Smith, J.E., Castellano, M.A., Miller, S.L. & Trappe, M.J. 2009. Diversity, Ecology and Conservation of Truffle Fungi in Forests of the Pacific Northwest. United States Department of Agriculture Forest Service Pacific Northwest Research Station General Technical Report PNW-GTR-772, USA, 202 pp.
  • 30. Türkoğlu, A. & Castellano, M.A. 2014. New records of some Ascomycete truffle fungi from Turkey. Turkish Journal of Botany, 38: 406-416.
  • 31. Türkoğlu, A., Castellano, M.A., Trappe, J.M. & Yaratanakul, G.M. 2015. Turkish truffles I: 18 new records for Turkey. Turkish Journal of Botany, 39: 359-376.
  • 32. Uzun, Y. & Yakar, S. 2018. New locality record for two Tuber species in Turkey. Anatolian Journal of Botany, 2(2): 88-92.
  • 33. Vezzola, V. 2005. Primi Risultati Produttivi Con Piante Micorrizate da T. macrosporum Vittad., 51-55, Paper presented at the Atti Seminario Sullo Stato Attuale Della Artuficoltura Italiana. 21 Febbraio, Spoleto-Italy.
  • 34. White, T.J., Bruns, T., Lee, S. & Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322. In: Innis M.A., Gefland D.H., Sninsky J.J. & White T.J. (eds). PCR Protocols: A Guide to Methods and Applications. Academic Press Inc., New York, 482 pp.

A NEW TRUFFLE SPECIES ADDITION, Tuber macrosporum Vittad., TO TURKISH MYCOTA

Year 2021, Volume: 22 Issue: 2, 139 - 146, 15.10.2021
https://doi.org/10.23902/trkjnat.873651

Abstract

Tuber P. Micheli ex F.H. Wigg. species have always attracted people's attention, with their high diversity, culinary and economic interest, strong aromas as well as the importance of plant ecosystem and animal nutrition. Interest in truffle species has been increasing in recent years in Turkey. Although some truffle species have been known previously in Turkey, many species are yet to be identified. Tuber macrosporum Vittad. samples were collected from Edirne and Tekirdağ regions in 2017 in a field study conducted to find new truffle species. Tuber macrosporum samples were firstly identified by macro and microscopic features, and this result was supported as 99% by DNA analyses when compared to GenBank.
A short description of the newly reported species is given along with its macro and microphotographs, and spore images taken by a scanning electron microscope (SEM). Additionally, ITS based evolutionary history of the species is provided with phylogenetic trees.

Supporting Institution

Selçuk University, Scientific Research Projects Coordinating Office

Project Number

BAP/20401081

Thanks

I would like to thank Ziyafet Arslan for his valuable effort he made during the field studies.

References

  • 1. Akata, I., Sevindik, M. & Şahin, E. 2020. Tuber fulgens Quél. A New record for Turkish truffles. Turkish Journal of Agriculture-Food Science and Technology, 8(11): 2472-2475.
  • 2. Alkan, S., Aktaş, S. & Kaşık, G. 2018. Türkiye’deki Tuber türleri ve Tuber aestivum için yeni bir lokalite. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, 44(1): 25-29.
  • 3. Astier, J. 1998. Truffes Blanches et Noires (Tuberaceae & Terfeziaceae). Louis-Jean, 127 pp.
  • 4. Bencivenga, M. & Baciarelli Falini, L. 2012. Manuale di Tartuficoltura. Esperienze di Coltivazionedei Tartufi in Umbria. Assessorato Regionale Agricoltura e Foreste, Regione Umbria, 137 pp.
  • 5. Benucci, G.M.N., Goga Csorbai, A., Di Massimo, G., Baciarelli Falini, L., Bencivenga, M. & Donini, D. 2012. Mycorrhization of Quercus robur L. and Corylus avellana L. seedlings with Tuber macrosporum Vittad. Mycorrhiza, 22(8): 639-646.
  • 6. Breitenbach, J. & Kränzlin, F. 1983. Fungi of Switzerland: Vol.1. Ascomycetes. Verlag Mykologia, Luzern, 310 pp.
  • 7. Castellano, M.A. & Türkoğlu, A. 2012. New records of truffle taxa in Tuber and Terfezia from Turkey. Turkish Journal of Botany, 36(3): 295-298.
  • 8. Elliott, T.F., Türkoğlu, A., Trappe, J.M. & Yaratanakul, G.M. 2016. Turkish truffles 2: eight new records from Anatolia. Mycotaxon, 131: 439-453.
  • 9. Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39: 783-791.
  • 10. Gezer, K., Kaygusuz, O., Çelik, A. & Işıloğlu, M. 2014. Ecological characteristics of truffles growing in Denizli Province, Turkey. Journal of Food, Agriculture & Environment, 12(2): 1105-1109.
  • 11. Gori, L. 2005. Funghi Ipogei Della Lucchesia di Aitre Province Italiane e dall' Estero. Pacini Fazzi, 316 pp.
  • 12. Granetti, B., De Angelis, A. & Materozzi, G. 2005. Umbria, Terra di Tartufi. Regione Umbria-Gruppo Micologico Ternano, Terni, 303 pp.
  • 13. Hall, I., Brown, G. & Zambonelli, A. 2007. Taming the Truffle. The history, Lore, and Science of the Ultimate Mushroom. Timber Press, Oregon, 304 pp.
  • 14. Kaya, A. 2009. Macromycetes of Kahramanmaraş province (Turkey). Mycotaxon, 108: 31-34.
  • 15. Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 111-120.
  • 16. Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evoluation, 35: 1547-1549.
  • 17. Ławrynowicz, M., Krzyszczyk, T. & Fałdziński, M. 2008. Occurrence of black truffles in Poland. Acta Mycologica, 43(2): 143-151.
  • 18. Marjanović, Ž., Grebenc, T., Marković, M., Glišić, A. & Milenković, M. 2010. Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula. Sydowia, 62(1): 67-87.
  • 19. Medardi, G. 2006. Ascomiceti d’Italia Atlante Fotografico. Fondazione Centro Studi Micologici Dell’A.M.B., Vicenza, 678 pp.
  • 20. Medardi, G. 2012. Atlante Fotografico Degli Ascomiceti d’Italia. Fondazione Centro Studi Micologici Dell’A.M.B. Vicenza, 454 pp.
  • 21. Montecchi, A. & Sarasini, M. 2000. Funghi Ipogei d'Europa. Associazione Micologica Bresadola, Trento, 714 pp.
  • 22. Öztürk, C., Kaşık, G. & Toprak, E. 1997. Ascomycetes makrofunguslarından Türkiye için iki yeni kayıt. Ot Sistematik Botanik Dergisi, 4: 53-56.
  • 23. Pegler, D.N., Spooner, B.M. & Young, T.W.K. 1993. British Truffles: A revision of British Hypogeous fungi. The Royal Botanic Gardens, Kew, 216 pp.
  • 24. Sesli, E., Asan, A., Selçuk, F. (eds.), Abacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H.H., Erdoğdu, M., Ergül, C.C., Eroğlu, G., Giray, G., Haliki Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Ya., Uzun, Yu. & Yoltaş, A. (2020). Türkiye Mantarları Listesi. Ali Nihat Gökyiğit Vakfı Yayını. İstanbul, 1192 pp.
  • 25. Şen, I., Allı, H. & Civelek, H.S. 2016. Checklist of Turkish truffles. Turkish Journal of Life Sciences, 1(2): 103-109.
  • 26. Stobbe, U., Büntgen, U., Sproll, L., Tegel, W. & Egli, S. 2012. Spatial distribution and ecological variation of re-discovered German truffle habitats. Fungal Ecology, 5(5): 591-599.
  • 27. Thompson, P.I. 2013. Ascomycetes in Colour: Found and Photographed in Mainland Britain. Xlibris, Lexington KY, 408 pp.
  • 28. Trappe, M., Evans, F. & Trappe, J. 2007. Field Guide to North American Truffles: Hunting, Identifying, and Enjoying the World's Most Prized Fungi. Ten Speed Press, Berkeley, Toronto, 136 pp.
  • 29. Trappe, J.M., Molina, R., Luoma, D.L., Cázares, E., Pilz, D., Smith, J.E., Castellano, M.A., Miller, S.L. & Trappe, M.J. 2009. Diversity, Ecology and Conservation of Truffle Fungi in Forests of the Pacific Northwest. United States Department of Agriculture Forest Service Pacific Northwest Research Station General Technical Report PNW-GTR-772, USA, 202 pp.
  • 30. Türkoğlu, A. & Castellano, M.A. 2014. New records of some Ascomycete truffle fungi from Turkey. Turkish Journal of Botany, 38: 406-416.
  • 31. Türkoğlu, A., Castellano, M.A., Trappe, J.M. & Yaratanakul, G.M. 2015. Turkish truffles I: 18 new records for Turkey. Turkish Journal of Botany, 39: 359-376.
  • 32. Uzun, Y. & Yakar, S. 2018. New locality record for two Tuber species in Turkey. Anatolian Journal of Botany, 2(2): 88-92.
  • 33. Vezzola, V. 2005. Primi Risultati Produttivi Con Piante Micorrizate da T. macrosporum Vittad., 51-55, Paper presented at the Atti Seminario Sullo Stato Attuale Della Artuficoltura Italiana. 21 Febbraio, Spoleto-Italy.
  • 34. White, T.J., Bruns, T., Lee, S. & Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322. In: Innis M.A., Gefland D.H., Sninsky J.J. & White T.J. (eds). PCR Protocols: A Guide to Methods and Applications. Academic Press Inc., New York, 482 pp.
There are 34 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Article/Araştırma Makalesi
Authors

Hasan Dogan 0000-0001-8859-0188

Project Number BAP/20401081
Publication Date October 15, 2021
Submission Date February 3, 2021
Acceptance Date May 15, 2021
Published in Issue Year 2021 Volume: 22 Issue: 2

Cite

APA Dogan, H. (2021). A NEW TRUFFLE SPECIES ADDITION, Tuber macrosporum Vittad., TO TURKISH MYCOTA. Trakya University Journal of Natural Sciences, 22(2), 139-146. https://doi.org/10.23902/trkjnat.873651
AMA Dogan H. A NEW TRUFFLE SPECIES ADDITION, Tuber macrosporum Vittad., TO TURKISH MYCOTA. Trakya Univ J Nat Sci. October 2021;22(2):139-146. doi:10.23902/trkjnat.873651
Chicago Dogan, Hasan. “A NEW TRUFFLE SPECIES ADDITION, Tuber Macrosporum Vittad., TO TURKISH MYCOTA”. Trakya University Journal of Natural Sciences 22, no. 2 (October 2021): 139-46. https://doi.org/10.23902/trkjnat.873651.
EndNote Dogan H (October 1, 2021) A NEW TRUFFLE SPECIES ADDITION, Tuber macrosporum Vittad., TO TURKISH MYCOTA. Trakya University Journal of Natural Sciences 22 2 139–146.
IEEE H. Dogan, “A NEW TRUFFLE SPECIES ADDITION, Tuber macrosporum Vittad., TO TURKISH MYCOTA”, Trakya Univ J Nat Sci, vol. 22, no. 2, pp. 139–146, 2021, doi: 10.23902/trkjnat.873651.
ISNAD Dogan, Hasan. “A NEW TRUFFLE SPECIES ADDITION, Tuber Macrosporum Vittad., TO TURKISH MYCOTA”. Trakya University Journal of Natural Sciences 22/2 (October 2021), 139-146. https://doi.org/10.23902/trkjnat.873651.
JAMA Dogan H. A NEW TRUFFLE SPECIES ADDITION, Tuber macrosporum Vittad., TO TURKISH MYCOTA. Trakya Univ J Nat Sci. 2021;22:139–146.
MLA Dogan, Hasan. “A NEW TRUFFLE SPECIES ADDITION, Tuber Macrosporum Vittad., TO TURKISH MYCOTA”. Trakya University Journal of Natural Sciences, vol. 22, no. 2, 2021, pp. 139-46, doi:10.23902/trkjnat.873651.
Vancouver Dogan H. A NEW TRUFFLE SPECIES ADDITION, Tuber macrosporum Vittad., TO TURKISH MYCOTA. Trakya Univ J Nat Sci. 2021;22(2):139-46.

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