Effects of Melatonin on Some Antioxidant Enzymes in Streptozotocin-induced Diabetic Rats

Year 2019, Volume: 21 Issue: 2, 217 - 222, 15.09.2019

Deniz Uluışık , Ercan Keskin , Durmuş Hatipoğlu

https://doi.org/10.15314/tsed.560865

Cited By: 1

Abstract

In this study, it was aimed to determine the
effects of melatonin application in experimentally induced diabetes. For this
purpose, thirty two adult male Wistar Albino rats were used. Animals in control
group were not any treatment. Melatonin group animals received 50 mg/kg
melatonin intraperitoneally in daily for eight weeks. Diabetes was induced by
subcutaneous injections of streptozotocin at dose of 40 mg/kg for two days as a
single dose per day in diabetes group animals. Animals in Diabetes+Melatonin
group were made diabetic by streptozotocin
in the same way and then these animals received 50 mg/kg melatonin
intraperitoneally in daily for eight weeks. In blood
samples taken from all animals, insulin, glucose, HbA1c, MDA, GSH, SOD were determined. The insulin level in diabetes
group was lower than control group, while blood glucose level was higher
(p<0.05). Melatonin treatment to diabetic animals resulted in significantly
differences in these parameters compared to diabetes group (p<0.05). HbA1c
in diabetes group was higher than control group (p<0.05). Melatonin
application to diabetic animals significantly decreased HbA1c compared to diabetes
group (p<0.05). In diabetes group, GSH and SOD levels were found to be lower
than control group, whereas MDA level were high (p<0.05). The changes in GSH
and SOD levels with melatonin application to diabetic animals were not
important compared to diabetes group, while MDA level was significantly reduced
(p<0.05). As a result, we concluded that the melatonin treatment to
experimentally induced diabetic rats obviously improved the some negative
changes in metabolic parameters resulting from diabetes.

Keywords

Diabetes, melatonin, antioxidant, HbA1c

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