HPLC analyses of polyphenolic compounds in oak (Querces frainetto) honey from Kırklareli region of Turkey

Year 2019, Volume: 1 Issue: 1, - , 11.12.2019

Yakup Kara , Zehra Can , Sevgi Kolaylı

Abstract

In this study, phenolic profile and total
phenolic contents of oak honeys, a kind of dark honeydew honey, was determined.
 The methanolic honey extract was
enriched by liquid; liquid extraction with diethyl ether and ethyl acetate, and
then was analyzed by RP-HPLC-UV with acetonitrile: water mobile phase. Nineteen
phenolic standards were used to prepare calibration graphics. Seven phenolic
acid (gallic acid, protocatequic acid, p-OH benzoic acid, caffeic acid,
syringic acid, p-coumaric acid, ferulic acid) and twelve flavanoids
(catechin, epicatechin, rutin, myerecetin, resveratrol, daidzein, luteolin, t-cinnamic
acid, hesperetin, chrysin, pinocembrin, caffeic acid phenlyester (CAPE) were
used.  Total phenolic contents of the
honeys were measured by Folic Ciocalteau's assay.  All of the polyphenols except epicatechin,
rutin, luteolin and hesperetin were detected in varying amounts of the honey
samples. Protocatequic acid, ferulic acid, myricetin and chrysin were the most
abundant phenolic compounds. Total phenolic contents of the honey were from 54
to 88 mg GAE/100 g.  In summary, oak
honey has a high apitherapeutic value with rich polyphenol diversity.

Keywords

Antioxidant, Oak Honey

References

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