Determination of Ethylenediaminetetraacetic acid (EDTA) levels in surface waters by high performance liquid chromatography (HPLC)-Ultraviolet/Visible (UV/VIS) detector

Year 2022, Volume: 4 Issue: 2, 76 - 79, 29.12.2022

Oltan Canlı , Barış Güzel , Kartal Çetintürk

https://doi.org/10.51435/turkjac.1124687

Cited By: 1

Abstract

Ethylenediaminetetraacetic acid (EDTA) is a chemical that is harmful to human health with its high solubility in water, which is used as a metal chelating agent in industries such as medicine, food, personal care product, agriculture. Thus, it is necessary to should be monitored in surface waters taken from dams supplying drinking and utility water. This work presents the applicability of the HPLC-UV/VIS system for the quantification of EDTA in surface waters based on the limit values of national and international legislation such as the Turkish Regulation on the Management of Surface Water Quality. The applicability of EDTA quantification in surface water was checked with validation study. The method validation consisted of selectivity, calibration curve linearity, limit of detection (LOD) and limit of quantification (LOQ), accuracy (recovery), and precision. In selectivity study, no peaks belonging to interfering compounds that would cause false-positive results were found in the chromatograms at the retention time of EDTA (5.773 min.). The linearity of EDTA was obtained ranging from 10 µg/L to 200 µg/L concentrations with the correlation coefficient of 0.9985 and the calibration curve equation of y=4659.4x-50223. The LOD and LOQ values of EDTA was 2.85 µg/L and 9.51 µg/L with the RSD of 5.36. In accuracy, the mean recovery of EDTA in surface water has been determined as 87.51 percent with an RSD of 6.11. The repeatability (RSD, %) varied from 5.44 % to 7.02 % with concentrations of 35.19 ± 1.91 μg/L and 17.11 ± 1.20 μg/L, whereas the reproducibility (RSD, %) was obtained at 3.45 % with the concentration of 34.13 ± 1.18 μg/L. In this study, the presence of EDTA was investigated in approximately 300 surface water samples and EDTA was found as positive in the concentration range of 11.17 µg/L to 52.14 µg/L in eleven real samples.

Keywords

EDTA, HPLC, Pollution, Water

Thanks

The authors would like to thank Hüseyin Demir for his support in the laboratory studies.

References

• Referans1. R. Kubota, M. Tahara, K. Shimizu, N. Sugimoto, T. Nishimura, Determination of EDTA in water samples by SPE-gas chromatography/mass spectrometry, J. Water Environ Technol, 8, 2010, 347-353.
• Referans2. P. Kuran, D. Pilnaj, M. Dzurkova, M. Smaha, Method development for determination of EDTA in water by using traditional split/splitless injector – Comparing external and internal standard methods of quantification, IOP Conf Ser: Earth Environ Sci, 221, 2019, 012126.
• Referans3. F.G. Kari, W. Giger, Speciation and fate of ethylenediaminetetraacetate (EDTA) in municipal wastewater treatment, Water Res, 30, 1996, 122-134.
• Referans4. T.P. Knepper, A. Werner, G. Bogenschütz, Determination of synthetic chelating agents in surface and wastewater by ion chromatography-mass spectrometry, J. Chromatogr. A, 1085, 2005, 240-246.
• Referans5. C. Oviedo, J. Rodríguez, EDTA: The chelating agent under environmental scrutiny, Quim Nova, 26(6), 2003, 901–905.
• Referans6. SWR, The Turkish Regulation on the Management of Surface Water Quality, 2012, Official Gazette: 30.11.2012. Number: 28483 (in Turkish).
• Referans7. WFD, Council Directive 2008/105/EC of the European Parliament and of the Council of 16 December 2008 on Environmental Quality Standards in the Field of Water Policy, 2008.
• Referans8. T. Kemmei, S. Kodama, T. Muramoto, H. Fujishima, A. Yamamoto, Y. Inoue, K. Hayakawa, Study of solidphase extraction for the determination of sequestering agents in river water by high-performance liquid chromatography, J Chromatogr A, 1216, 2009, 1109-1114.
• Referans9. K.H. Bauer, T.P. Knepper, A. Maes, V. Schatz, M. Voihsel, Analysis of polar organic micropollutants in water with ion chromatography - electrospray mass spectrometry, J Chromatogr A, 837, 1999, 117-128.
• Referans10. Y. Nishikawa, T. Okumura, Determination of nitrilotriacetic acid and ethylenediaminetetraacetic acid in environmental samples as their methyl ester derivatives by gas chromatography-mass spectrometry, J Chromatogr A, 690, 1995, 109-118.
• Referans11. J.B. Quintana, T. Reemtsma, Rapid and sensitive determination of ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid in water samples by ion-pair reversed-phase liquid chromatography-electrospray tandem mass spectrometry, J Chromatogr A, 1145, 2007, 110-117.
• Referans12. ISO, Guide to the Expression of Uncertainty in Measurements. International Organization for Standardization (ISO), Geneva, Switzerland, 1993. https://www.iso.org/standard/ 45315.html, Accessed January 1993.
• Referans13. EURACHEM, The Fitness for Purpose of Analytical Methods: A Laboratory Guide to Method Validation and Related Topics. 2nd edn., LGC, Teddington, 2014. https://www.eurachem.org/ index.php/ publications/guides/mv
• Referans14. AOAC, Guidelines for Standard Method Performance Requirements. Appendix F., Association of Official Analytical Chemists, Virginia, USA, 2016. http://www.eoma.aoac.org/app_f.pdf
• Referans15. J.A. Ferreira, J.M.S Ferreira, V. Talamini, Determination of pesticides in coconut (Cocos nucifera Linn.) water and pulp using modified QuEChERS and LC-MS/MS. Food Chem, 213, 2016, 616-624.
• Referans16. B. Güzel, O. Canlı, Method validation and measurement uncertainty of possible thirty volatile organic compounds (VOCs) presented in the polyethylene present in bottled drinking waters sold in Turkey, J Anal Sci Technol, 11 (44), 2020, 1–17.
• Referans17. B. Güzel, O. Canlı, E. Oktem-Olgun, Gas chromatography method validationstudy for sensitive and accurate determination of volatile aromatic hydrocarbons (VAHs) in water, Cumhuriyet Sci J, 39 (4), 2018, 970–982.
• Referans18. B. Güzel, O. Canli, Applicability of purge & trap GC‐MS method for sensitive analytical detection of naphthalene and its derivatives in waters, J Mass Spectrom 55, 2020, 12.