Şeytan Elması (Datura stramonium L.) ve Sirken (Chenopodium album L.) Etanol Ekstraktlarının Bitki Patojeni (Macrophomina phaseolina ve Rhizoctonia solani) Fungusları Üzerine Allelopatik Etkileri

Year 2024, Volume: 11 Issue: 2, 389 - 395, 30.04.2024

Ayhan Taştan Enes Fidan , İlhan Kaya Tekbudak

https://doi.org/10.30910/turkjans.1429355

Cited By: 1

Abstract

Bu çalışmada şeytan elması (Datura stramonium L.) ve sirken (Chenopodium album L.)’den elde edilen etanol ekstraktlarının toprak kökenli bitki patojeni olan Macrophomina phaseolina ve Rhizoctonia solani AG3 fungusları üzerine etkileri araştırılmıştır. Bu patojenlerin mücadelesinde bitki koruma ürünlerine alternatif olabilecek bitkilerde sentezlenen doğal bileşikler ile insan ve çevreye duyarlı yöntemlerin geliştirilmesi amaçlanmıştır. Çalışmalar tesadüf parselleri deneme desenine göre beş tekrarlı olarak Patates dekstroz agar (PDA) besi ortamında 9 cm’lik petri kaplarında in vitro koşullarda yürütülmüştür. Negatif kontrol için Captan 50 içerikli fungisit, pozitif kontrol için ise %0 saf su kullanılmıştır. Toplanan şeytan elması ve sirken bitkilerinden elde edilen etanol ektraktları %2, 4, 8, 16 ve 32 konsantrasyonlarında uygulanmıştır. Söz konusu test patojenlerinin ekimi gerçekleştirilmiş ve 24±1°C’ye ayarlı inkübatörlere gelişim için bırakılmıştır. Yedinci günün sonunda bu uygulamaların miseliyal gelişimleri ölçülerek değerlendirilmeye alınmıştır. Bu değerlendirmelere göre, şeytan elması ve sirkenden elde edilen etanol ekstraktlarının her iki patojenin gelişimini olumsuz etkilediği gözlenmiştir. Özellikle %8 üzerindeki etanol dozlarının her iki test patojeninde gelişimini tamamen engellediği tespit edilmiştir. Sonuç olarak, şeytan elması ve sirkenin etanol ekstraktlarının M. phaseolina ve R. solani AG3 etmenlerine karşı pestisitlere alternatif olabilmeleri açısından ümitvar olduğu düşünülmektedir.

Keywords

Allelopati, Datura stramonium L., Chenopodium album L., bitki ekstrakları, fitopatojen fungus.

Allelopathic Effect Of Jimson Weed (Datura stramonium L.) and Lamb's Quarters (Chenopodium album L.) Ethanol Extracts On Some Plant Pathogen (Macrophomina phaseolina Ve Rhizoctonia solani) Fungi

Abstract

In this study were investigated the effects of ethanol extracts from jimson weed (Datura stramonium L.) and lamb's quarters (Chenopodium album L.) on soilborne plant pathogens Macrophomina phaseolina and Rhizoctonia solani AG3. It is aimed to develop methods that are sensitive to humans and the environment with natural compounds synthesized in plants that can be an alternative to pesticides in the control against these pathogens. The studies were conducted in vitro conditions in 9 cm petri dishes on Potato dextrose agar (PDA) medium, with five replicates, according to the randomized plot design. While Captan 50 fungicide was used for negative control, 0% pure water was applied for positive control. Ethanol extracts obtained from collected jimson weed and lamb's quarters plants were used at concentrations of 2, 4, 8, 16 and 32%. These test pathogens were inoculated and placed in incubators set at 24±1°C for growth. The mycelial development of these applications was measured and evaluated at the end of the seventh day. According to these evaluations, it was observed that ethanol extracts obtained from jimson weed and lamb's quarters negatively affected the development of both pathogens. In particular, ethanol doses above 8% were found to completely inhibit the growth of both test pathogens. Therefore, it is thought that there is potential for ethanol extracts of devil’s apple and lamb's quarters to replace pesticides in the control of M. phaseolina and R. solani AG3.

Keywords

Allelopathy, Datura stramonium L., Chenopodium album L., plant extract, phytopathogenic fungus.

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