Biber Genotiplerinde Peroksidaz ve Katalaz İçeriklerinin Phytophthora capsici’ye Dayanıklılık Üzerine Etkileri

Year 2018, Volume: 5 Issue: 1, 65 - 71, 28.02.2018

Muhemet Zeki Karipçin , Emre Demirer Durak

https://doi.org/10.19159/tutad.358705

Cited By: 1

Abstract

Yerel
biber genotiplerinde Phytophthora capsici’ye
dayanıklılık amacıyla yürütülen bu çalışmada, 60 adet biber genotipi
kullanılmıştır. Araştırmada; kontrol olarak P.
capsici’ye dayanıklı CM 334 (Criollos de Morelos 334) genotipi seçilmiş, peroksidaz ve katalaz enzim
içerikleri araştırılan tüm materyallere iki inokulasyon uygulanmıştır. İlk
inokulasyon sonucunda CM 334 no’lu genotip ve 3 (P1),
13 (Urfa), 25 (UKST), 38 (Uİ), 48 (UKDT) ve 57 (ANKSB) no’lu yerli genotipler
ölmemiştir. İkinci inokulasyon sonucunda ise sadece CM 334 no’lu genotip
hayatta kalarak sürgün oluşumuna devam etmiştir. Her iki inokulasyonda da kısmi
veya tam dayanıklılık gösteren genotiplerin enzim içeriklerinin de yüksek
seviyelerde olduğu, özellikle peroksidaz enzim içeriğinin belirgin bir şekilde
ayırıcı olduğu sonucuna varılmıştır. İlk inokulasyonda dayanıklılık gösteren
tüm genotiplerin peroksidaz içeriği yüksek olarak belirlenirken, katalaz içeriği
dayanıklı ve hassas çeşitlerde çok belirgin olarak ortaya çıkmamıştır. Ayrıca, P. capsici’ye dayanıklılığın gelişmenin ilk evrelerinden daha çok, bitki
gelişiminin son evrelerinde ortaya çıktığı belirlenmiştir. Çalışma sonucunda,
ilk inokulasyonda dahi kısmi dayanıklılık gösteren materyallerin ıslah
materyalleri olabileceği ve melezleme programlarına dâhil edilebileceği tespit
edilmiştir. 

Keywords

Biber, Phytophthora capsici, peroksidaz, katalaz

The Effects of Peroxidase and Catalase Components of Pepper Genotypes on Phytophthora capsici Resistance

Abstract

In this study, sixty landrace pepper genotypes were used to test the resistance to Phytophthora capsici. The CM
334 (Criollos de Morelos 334) P. capsici resistant genotype was selected as the control, and two inoculations were applied to
all the materials evaluated for peroxidase and catalase enzyme contents. As a result of the first inoculation, genotype CM
334 and native genotypes 3 (P1), 13 (Urfa), 25 (UKST), 38 (UD), 48 (UKDT) and 57 (ANKSB) survived. As a result of the
second (last) inoculation, only the CM 334 genotype survived and continued to form shoots. In both inoculations, it was
concluded that the enzyme contents of genotypes showing partial or complete resistance were also at elevated levels,
especially the peroxidase enzyme content was specifically distinctive. Peroxidase content of all genotypes showing
resistance in the first inoculation was high, but catalase content was not very apparent in resistant and sensitive varieties. It
has also been determined that P. capsici resistance appeared in the last stages of plant development, rather than in the initial
stages of development. As a result of the study, it was determined that materials showing partial resistance even in the first
inoculation, could be breeding materials and used in hybridization programs.

Keywords

Pepper, Phytophthora capsici, peroxidase, catalase

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