Investigation of Antidiabetic, Antimicrobial Effects and Phytochemical Composition of The Species From Stenocephalae Bunge. Section

Year 2023, Volume: 10 Issue: 1, 11 - 17, 14.04.2023

Leyla Paşayeva , Ufuk İnce , Havva Karadeli , Funda Maraşlı , Dilbeste Öztürk , Selin Urmak , Osman Tugay

https://doi.org/10.19159/tutad.1181413

Abstract

The aim of this study is to examine the hypoglycemic and antimicrobial effects of Cousinia davisiana, C. foliosa, C. ramosissima, and C. stenocephala species, which are included in the Stenocephalae section. Within the scope of the study, after the above-ground parts of the plants were collected, their extracts were prepared by maceration at room temperature with methanol, and the α-amylase, α-glucosidase inhibitory, and antimicrobial effects of these extracts were determined. When the results were evaluated, among the plants, the C. ramosissima extract was found to be more active in α-amylase with 246.784±1.528 µg mL-1 values compared to other extracts and higher α-amylase activity and α-glucosidase (155.458±0.279 µg mL-1) effect compared to acarbose. It was also found that the extracts showed moderate antimicrobial activity. Apart from this, it was determined that the active extract contained phenolic compounds and flavonoids intensely as a result of the examination by LC-MS/MS. Although the results of this study prove for the first time that especially C. ramosissima extract has effective α-amylase and α-glucosidase inhibitory effects, further studies are needed to show that these extracts can help reduce postprandial glucose levels.

Keywords

Cousinia, Asteraceae, hypoglycemic effect, phenolic compounds, flavonoids

Stenocephalae Bunge. Seksiyonuna Dâhil Olan Türlerin Antidiyabetik, Antimikrobiyal Etkisi ve Fitokimyasal İçeriğinin İncelenmesi

Abstract

Bu çalışmanın amacı, Stenocephalae seksiyonuna dâhil olan Cousinia davisiana, C. foliosa, C. ramosissima ve C. stenocephala türlerinin hipoglisemik ve antimikrobiyal etkisinin incelenmesidir. Çalışma kapsamında bitkilerin toprak üstü kısmı toplandıktan sonra metanol ile oda sıcaklığında maserasyon yöntemiyle ekstreleri hazırlanmış ve bu ekstrelerin α-amilaz, α-glikozidaz inhibitör ve antimikrobiyal etkisi tayin edilmiştir. Elde edilen sonuçlar değerlendirildiğinde bitkiler arasında C. ramosissima ekstresinin 246.784±1.528 µg mL-1 IC50 değeri ile diğer ekstrelere ve akarboza göre daha yüksek α-amilaz etki ve α-glikozidaz (155.458±0.279 µg mL-1) etki, ayrıca çalışılan ekstrelerin orta derecede antimikrobiyal etki gösterdiği bulunmuştur. Bunun dışında aktif bulunan ekstrenin LC-MS/MS ile yapılan inceleme sonucunda fenolik bileşikler ve flavonoitleri yoğun olarak içerdiği belirlenmiştir. Bu çalışmanın sonuçları ile özellikle C. ramosissima ekstresinin etkili α-amilaz ve α-glikozidaz inhibitör etkiye sahip olduğu ilk kez kanıtlanmakla birlikte, bu ekstrelerin postprandiyal glikoz düzeylerini azaltmaya yardımcı olabileceğini göstermek için daha ileri çalışmalara ihtiyaç duyulmaktadır.

Keywords

Cousinia, Asteraceae, hipoglisemik etki, fenolik bileşikler, flavonoitler

References

• Ali, H., Houghton, P., Soumyanath, A., 2006. α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. Journal of Ethnopharmacology, 107(3): 449-455.
• Amiri, M.S., Hosseini, H.A., Rajai, P., 2014. Preliminary investigation on phytochemical composition and anti-bacterial activity of the root of Cousinia microcarpa Boiss. Asian Journal of Medical and Pharmaceutical Researches, 4(4): 156-159.
• Anonymous, 2008. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard. Clinical and Laboratory Standards Institute Document, M27-A3 and Supplement S, 3, 6-12.
• Anonymous, 2021. Performance Standards for Antimicrobial Susceptibility Testing. Clinical and Laboratory Standards Institute.
• Behlil, F., Samiullah, K.N., Akbar, A., Tareen, R.B., Achakazai, A.K.K., Ali, I., Rehman, A., 2019. Phytochemical screening and antioxidant activity determination of some medicinally important plants of Balochistan. Pakistan Journal of Botany, 51(2): 1-8.
• Clifford, M.N., Johnston, K.L., Knight, S., Kuhnert, N., 2003. Hierarchical scheme for LC-MS n identification of chlorogenic acids. Journal of Agricultural and Food Chemistry, 51(10): 2900-2911.
• Dixon, W.J., Massey, Jr., Frank, J., 1951. Introduction to Statistical Analysis. McGraw-Hill.
• Funke, I., Melzig, M., 2005. Effect of different phenolic compounds on α-amylase activity: Screening by microplate-reader based kinetic assay. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 60(10): 796-797.
• González-Caballero, V., Pérez-Marín, D., López, M.-I., Sánchez, M.-T., 2011. Optimization of NIR spectral data management for quality control of grape bunches during on-vine ripening. Sensors, 11(6): 6109-6124.
• Gouveia, S.C., Castilho, P.C., 2012. Phenolic composition and antioxidant capacity of cultivated artichoke, Madeira cardoon and artichoke‐based dietary supplements. Food Research International, 48(2): 712-724.
• Hasenah, P., Houghton, J., Soumyanath, A., 2006. α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. Journal of Ethnopharmacology, 10(3): 449-455.
• Huber-Morath, A., 1975. Cousinia. Flora of Turkey and the East Aegean Islands, 5: 329-353.
• Meng, Y., Su, A., Yuan, S., Zhao, H., Tan, S., Hu, C., Deng, H., Guo, Y., 2016. Evaluation of total flavonoids, myricetin, and quercetin from Hovenia dulcis Thunb. as inhibitors of α-amylase and α-glucosidase. Plant Foods for Human Nutrition, 71(4): 444-449.
• Oboh, G., Ademosun, A.O., Ayeni, P.O., Omojokun, O.S., Bello, F., 2015a. Comparative effect of quercetin and rutin on α-amylase, α-glucosidase, and some pro-oxidant-induced lipid peroxidation in rat pancreas. Comparative Clinical Pathology, 24(5): 1103-1110.
• Oboh, G., Agunloye, O.M., Adefegha, S.A., Akinyemi, A. J., Ademiluyi, A.O., 2015b. Caffeic and chlorogenic acids inhibit key enzymes linked to type 2 diabetes (in vitro): a comparative study. Journal of Basic and Clinical Physiology and Pharmacology, 26(2): 165-170.
• Paşayeva, L., Eruygur, N., Ayaz, F., Tugay, O., Fatullayev, H., 2021. Assessment of the antioxidant and enzyme inhibition activities of Cousinia iconica with focus on phytochemical investigation by LC-MS/MS. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 155(6): 1178-1188.
• Paşayeva, L., Kararenk, A.C., Koşar, M., 2020. Cousinia Cass. cinsinin geleneksel kullanimi, fitokimyasal içeriği ve biyolojik etkileri. Journal of Faculty of Pharmacy of Ankara University, 44(2): 293-307.
• Riethmüller, E., Alberti, Á., Tóth, G., Béni, S., Ortolano, F., Kéry, Á., 2013. Characterisation of diarylheptanoid‐and flavonoid‐type phenolics in Corylus avellana L. leaves and bark by HPLC/DAD–ESI/MS. Phytochemical Analysis, 24(5): 493-503.
• Saldanha, L.L., Vilegas, W., Dokkedal, A.L., 2013. Characterization of flavonoids and phenolic acids in Myrcia bella cambess. Using FIA-ESI-IT-MSn and HPLC-PAD-ESI-IT-MS combined with NMR. Molecules, 18(7): 8402-8416.
• Salimi-Sabour, E., Shirazi, F.H., Mahboubi, A., Mojab, F., Irani, M., 2021. Biological Activities and the Essential Oil Analysis of Cousinia harazensis and C. calocephala. Iranian Journal of Pharmaceutical Research, 20(3): 140.
• Shahverdi, A.R., Khorramizadeh, M.R., Attar, F., Saadat, F., Vahid, S., Ghahraman, A., 2007. Concomitant chemopreventive and antibacterial effects of some Iranian plants from the genus Cousinia (Asteraceae). Revista Brasileira de Farmacognosia, 17: 325-330.
• Shi, L., Ge, H.-M., Tan, S.-H., Li, H.-Q., Song, Y.-C., Zhu, H.-L., Tan, R.-X., 2007. Synthesis and antimicrobial activities of Schiff bases derived from 5-chloro-salicylaldehyde. European Journal of Medicinal Chemistry, 42(4): 558-564.
• Simirgiotis, M.J., Schmeda-Hirschmann, G., 2010. Determination of phenolic composition and antioxidant activity in fruits, rhizomes and leaves of the white strawberry (Fragaria chiloensis spp. chiloensis form chiloensis) using HPLC-DAD–ESI-MS and free radical quenching techniques. Journal of Food Composition and Analysis, 23(6): 545-553.
• Singh, K.N., 2012. Traditional knowledge on ethnobotanical uses of plant biodiversity: a detailed study from the Indian western Himalaya. Biodiversity Research and Conservation, 28: 63.
• Skelin, M., Rupnik, M., Cencič, A., 2010. Pancreatic beta cell lines and their applications in diabetes mellitus research. ALTEX-Alternatives to Animal Experimentation, 27(2): 105-113.
• Yılmaz, F.K., Paşayeva, L., Tugay, O., 2022. LC–ESI-MS/MS-based phytochemical profile, antioxidant and enzyme inhibition activities of extracts from Cousinia aintabesis Boiss. & Hausskn. Farmacia, 70(2): 287-294.