ÜLKEMİZDE GÜNEY MARMARA BÖLGESİ’NDEKİ BAL ARILARINDA NOSEMA CERANAE’NİN MİKROSKOBİK VE MOLEKÜLER OLARAK BELİRLENMESİ

Year 2016, Volume: 16 Issue: 1, 20 - 26, 30.11.2016

İbrahim Çakmak

https://doi.org/10.31467/uluaricilik.379261

Cited By: 1

Abstract

Bu çalışmada Bursa ve Balıkesir çevresindeki kolonilerde mikroskobik olarak Nosema türlerinin
varlığını belirlemek ve multipleks PCR yöntemi ile kolonilerin hangi Nosema türü (N. ceranae ve N.
apis) ile enfekte olduğunu tespit etmek amaçlanmıştır. Mikroskobik inceleme sonucunda, 14
koloniden 11 tanesindeki (%78,6) bireylerde Nosema sporları bulunmuş ve taranan bireylerin Nosema
ile enfekte olduğu tespit edilmiştir. Bu kolonilerden alınan örneklerde multipleks PCR metodu
kullanılarak moleküler tanımlama ile N. ceranae’nın 16S rRNA gen bölgesini karakterize eden bantlar
bulunmuştur. Çalışmada kolonilerin büyük çoğunluğunun N. ceranae ile enfekte olduğu tespit
edilmiştir.

Keywords

Apis mellifera, Nosema apis, Nosema ceranae, tanımlama, Bursa ve Balıkesir

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