Effect of genetically modified gene tolerant dendritic cell therapy on some blood parameters in experimental mouse arthritis model

Year 2018, Volume:37 Number:2, 101 - 107, 15.10.2018

Gökçen Güvenç İzel Yılmaz Kübra Çiftçi , Ayşenur Baş , Esra Kaşıkcı Figen Ersoy , Haluk Barbaros Oral , Murat Yalçın

https://doi.org/10.30782/uluvfd.413321

Abstract

Rheumatoid
arthritis (RA) is an inflammatory autoimmune disease that causes chronic pain
and joint destruction. T cells activation has an important role in RA
pathogenesis. Activation of T lymphocytes requires the co-stimulatory signals
provided by antigen-presenting cells. T-cell activation without co-stimulation
results in anergy. In this study, to inhibit the activation of T lymphocytes
formed in the experimental arthritis, tolerogenic dendritic cells were aimed to
be obtained by the genetical modification of dendritic cells with CTLA4-KDEL overexpression in endoplasmic
reticulum. Then, RA created animals treated with tolDCs and the effect of
treatment on blood parameters were investigated.

For
this purpose, mouse collagen induced arthritis model was used. The mice with
arthritis were intraarticularly treated with modified tolDCs. It was observed
that the treatment group significantly reversed the increase in the joint
thickness and the increase in the number of white blood cells, especially with
the increase in neutrophils when compared with control groups.

As
a result, genetically modified tolDCs reduced the clinical symptoms of
experimental arthritis and also reversed the changes in blood parameters due to
experimental arthritis in mice.

Keywords

Experimental rheumatoid arthritis, CTLA4-KDEL gene therapy, Red blood cell count, Hemoglobine, Hematocrit, White blood cell count, Formula leucocyte

Effect of genetically modified gene tolerant dendritic cell therapy on some blood parameters in experimental mouse arthritis model

Abstract

Rheumatoid arthritis (RA) is an inflammatory autoimmune disease that causes chronic pain and joint destruction. T cells activation has an important role in RA pathogenesis. Activation of T lymphocytes requires the co-stimulatory signals provided by antigen-presenting cells. T-cell activation without co-stimulation results in anergy. In this study, to inhibit the activation of T lymphocytes formed in the experimental arthritis, tolerogenic dendritic cells were aimed to be obtained by the genetical modification of dendritic cells with CTLA4-KDEL overexpression in endoplasmic reticulum. Then, RA created animals treated with tolDCs and the effect of treatment on blood parameters were investigated.

For this purpose, mouse collagen induced arthritis model was used. The mice with arthritis were intraarticularly treated with modified tolDCs. It was observed that the treatment group significantly reversed the increase in the joint thickness and the increase in the number of white blood cells, especially with the increase in neutrophils when compared with control groups.

As a result, genetically modified tolDCs reduced the clinical symptoms of experimental arthritis and also reversed the changes in blood parameters due to experimental arthritis in mice.

Keywords

Deneysel romatoid artrit, CTLA4, tolDH, Alyuvar sayısı, Hemoglobin, Hematokrit, Akyuvar sayısı, Akyuvar formülü

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