Genotype frequency of FecXB (Belclare) mutation of BMP15 gene in Chios (Sakiz) sheep

Year 2018, Volume:37 Number:2, 87 - 91, 15.10.2018

Deniz Dinçel , Sena Ardıçlı , Hale Şamlı Faruk Balcı

https://doi.org/10.30782/uluvfd.413857

Cited By: 3

Abstract

Ovulation rate and litter
size, varies between breeds or individulas who belong to the same breed, are
evaluated as fertility traits. Fertility traits are important for economic
values in animal breeding. However these traits exhibit only in females and they
are determined in the later stages of lifes. Hence the genetic perspective is more
effective way to confirm the fertility capacities early period of life time.
Fecundity genes such as BMP15
regulate the fertility traits by increasing the ovulation rate due to the occuring
mutations. Despite some prolific breed were investigated for FecX^B mutation; the
researches carried out to determine thatmutation in Chios sheep breed are limited. The objective of the
current study was to estimate the the genotype frequencies of FecX^B mutation of BMP15 gene in prolific Chios sheep. We
investigated the FecX^B
mutation by PCR-RFLP method in Chios sheep (n=77). According to result, the
investigated Chios breed was found monomorphic for FecX^B mutation. All individuals were digested by DdeI restriction enzyme and showed wild-type
genotype and did not carry FecX^B
mutation nin present study. In conclusion it’s thought that the high
prolificacy in Chios sheep may be based on another region of BMP15 gene or different major gene. Thus
the effect of other major genes or regions/QTL’s should be investigated in
future studies with a large number of animals.

Keywords

Sheep, fertiliy, mutation, PCR-RFLP, BMP15 gene, Chios

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