First DNA Barcode of Bruchidius varius (Coleoptera: Chrysomelidae: Bruchinae) from Turkey

Year 2022, , 76 - 80, 29.12.2022

Gözde Uçan , Ali Nafiz Ekiz

https://doi.org/10.47137/usufedbid.1148641

Abstract

The DNA barcode of Bruchidius varius collected from Dilek Peninsula and Büyük Menderes Delta National Park (DPNP) in Aydın province of Turkey is given for the first time. As DNA barcode, mitochondrial cytochrome c oxidase subunit I (COI) with a length of approximately 628 bp is sequenced and an illustrative barcode is presented along with nucleotide composition of the target gene location. Nucleotide composition of the given sequence includes 38.63% T, 27.98% A, 17.01% C and 16.38% G and showing a strong AT bias (66.61%). GC percentage is 33.39%. BLAST tool in NCBI is used to check the taxonomic position of the sequenced sample. DNA barcoding result fully corresponds with the morphological identification of the samples.

Keywords

Seed beetles, Bruchidius varius, COI sequence, DNA barcode, nucleotide composition

Supporting Institution

TUBİTAK

Project Number

119Z544

Thanks

We sincerely thank to TUBİTAK for supporting this study (Project number: 119Z544).

Bruchidius varius'un (Coleoptera: Chrysomelidae: Bruchinae) Türkiye'den ilk DNA barkod kaydı

Abstract

Dilek Yarımadası ve Büyük Menderes Deltası Milli Parkı’ndan (Aydın) toplanmış Bruchidius varius türüne ait DNA barkodu ülkemizden ilk kez verilmiştir. DNA barkodu olarak yaklaşık 628 bp uzunluğundaki mitokondriyal sitokrom c oksidaz altünite 1 (COI) geni kullanılmıştır. Ayrıca ilgili gen bölgesi sekansının nükleotid kompozisyonu verilmiştir. Nükleotid komposizyonunda kuvvetli bir AT yönelimi ((%66,61) görülmüş olup nükleotid oranları %38,63 T, %27,98 A, %17,01 C ve %16,38 G şeklindedir. GC oranı %33,39’dur. Dizilenen örneğin taksonomik durumu NCBI’da bulunan BLAST aracıyla kontrol edilmiştir. DNA barkodlama sonucu, örneklerin morfolojik teşhisi ile örtüşmektedir.

Keywords

Tohum böcekleri, Bruchidius varius, COI sekansı, DNA barkodu, nükleotid kompozisyonu

Project Number

119Z544

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