Existence of Plasmidic AmpC Beta-Lactamase-Producing Escherichia coli Isolates in Healthy Laying Hens

Year 2017, Volume: 28 Issue: 2, 63 - 67, 20.08.2017

Faruk Pehlıvanoglu

Abstract

In the
present study, determination of prevalence
of plasmidic AmpC (pAmpC) beta-lactamase-producing Escherichia coli in intestinal flora of laying hens in Burdur
province of Turkey and characterization of pAmpC beta-lactamase-producing E. coli isolates were aimed. Two
hundreds twenty five fecal samples from all laying hen farms (n=4) in Burdur
province were collected and cultured in Brilliance E. coli/coliform selective agar supplemented with cefotaxime or
ceftazidime. Presumptive AmpC beta-lactamase-producing E. coli isolates were determined by a phenotypic test and the
isolates were screened by PCR for bla_pAmpC
genes. Susceptibilities of the E. coli
isolates to beta-lactams and other classes of antibiotics were investigated by
agar disc diffusion test and finally phylogenetic analysis of the E. coli isolates was performed by
multiplex PCR. pAmpC beta-lactamase-producing E. coli was isolated from 15 (6.7%) laying hen fecal samples. bla_CITM family gene was found
in all E. coli isolates. The pAmpC
beta-lactamase-producing isolates showed co-resistance to several classes of
antibiotics (aminoglycosides, quinolones, sulfamethoxazole-trimethoprim and
tetracycline). According to phylogenetic analysis, the E. coli isolates belonged to A₁, B1 and D₁
groups. Consequently, by the present study the first isolation of pAmpC
beta-lactamase-producing E. coli
isolates with multidrug-resistance phenotype on lying hen production from
Turkey was reported.

Keywords

Chicken, Escherichia coli, Multiple antibacterial drug resistance, AmpC beta-lactamases

Sağlıklı Yumurta Tavuklarında Plasmid Kökenli AmpC Beta Laktamaz üreten Escherichia coli Varlığı

Abstract

u
çalışmada, Burdur ilinde yetiştirilen yumurtacı tavukların bağırsak
mikroflorasında plazmid kökenli AmpC (pAmpC) beta laktamaz üreten Escherichia coli izolatlarının
prevalansını belirlemek ve belirlenen pAmpC beta laktamaz üreten E. coli izolatlarının
karakterizasyonunun yapılması amaçlandı.
Burdur ilindeki tüm yumurtacı tavuk çiftliklerinden (n=4) 225 dışkı örneği
toplandı ve içerisine sefotaksim veya seftazidim ilave edilerek hazırlanmış E. coli/koliform selektif agarda dışkı
örneklerinin kültürleri yapıldı. AmpC beta laktamaz üreten E. coli izolatlarının ön
tanısı fenotipik bir test ile yapıldı ve izolatlar bla_pAmpC beta laktamaz genleri için PZR ile tarandı. E. coli izolatlarının beta laktam ve
diğer sınıflardan antibiyotiklere olan duyarlılıkları agar disk difüzyon testi
ile araştırıldı ve son olarak E. coli
izolatlarının filogenetik analizi multipleks PZR ile gerçekleştirildi. pAmpC
beta laktamaz üreten E. coli 15 adet
(%6.7) dışkı örneğinden izole edildi. CIT familyasına ait bla_pAmpC geni (bla_CIT)
tüm E. coli izolatlarında bulundu.
pAmpC beta laktamaz üreten izolatlar çeşitli sınıflardan antibiyotiklere
(aminoglikozidler, kinolonlar, sulfametoksazol-trimethoprim ve tetrasiklinler)
karşı dirençli bulundu. Filogenetik analiz sonuçlarına göre E. coli izolatları A₁, B1 ve
D₁ gruplarına ait oldukları belirlendi. Sonuç olarak, Türkiye’de
yumurtacı tavuk üretiminde pAmpC beta laktamaz üreten ve çoklu antibiyotik
direnci gösteren E. coli
izolatlarının ilk kez bu çalışmayla ortaya konulduğu görüldü.

Keywords

Tavuk, Escherichia coli, Çoklu antibakteryel ilaç dirençliliği, AmpC beta laktamazlar

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