Abstract
The present study was aimed at investigating the pecten oculi of the
ostrich by light and electron microscopy. The pecten oculi of 5 healthy adult
ostriches, obtained from a slaughterhouse, constituted the material of the
study. When examined macroscopically, the pecten oculi of the ostrich was
observed to be a highly pigmented and relatively large fan-like shaped (vaned)
structure consisting of approximately 20-24 folds. It was situated on the
ventral surface of the eye and extended into the vitreous body. Light
microscopic investigation revealed two or more blood vessels and numerous
capillaries in each pecteneal fold. The capillaries had a thick basal lamina
and numerous melanocytes and the pecten was surrounded by the vitreo-pecteneal
limiting membrane. Hyalocytes were not observed outside of the vitreo-pecteneal
membrane. At transmission electron microscopic (TEM) examination, endothelial
cells were observed to possess a thin cytoplasm and the organelles were
localized to the center of the cells. The capillary endothelium exhibited a
large number of luminal and abluminal folds. Pericytes were seen lying beneath
the basal lamina. In the present study, ostrich pecten oculi was examined by
light and electron microscopic and compared with other poultry species and
their differences were revealed.
References
- Amemiya T (1985). Constituents of connective tissue around the capillary of chick pecten oculi. Acta Anat, 122, 235-238. Braekevelt CR (1984). Electron microscopic observation on the pecten oculi of the nighthawk. Ophthalmologica, 189, 211-220. Braekevelt CR (1988). Fine structure of the pecten oculi of the pigeon. Ophthalmologica, 196, 151-159. Braekevelt CR (1991). Electron microscopic observation on the pecten oculi of the gread blue heron. Histol Histopathol, 6, 345-351. Braekevelt CR (1991a). Fine structure of the pecten oculi in the red-tailed hawk. Anat Histol Embryol, 8, 9-15. Braekevelt CR (1993). Fine structure of the pecten oculi in the great horned owl. Histol Histopathol, 8, 9-15. Braekevelt CR (1994). Fine structure of the pecten oculi in the American crow. Anat. Histol Embryol, 23, 357-366. Braekevelt CR (1998). Fine structure of the pecten oculi of the emu. Tissue Cell, 30, 157-165. Dayan MO, Ozaydin TA (2013). Comparative morphomertical study of the pecten oculi in different avian species. Sci World J, Article ID: 968652 Denk H, Kunzele H, Plenk H, Ruschoff J, Sellner W (1989). Romeis Microscopische Tecnic, 17, Neubearbeitete Auflage. Urban und Schwarzenberg, München, Wien, Baltimore, p: 439-450. De Schaepdrijver L, Simoens P, Lauwers H, De Geest JP (1989). Retinal vascular patterns in domestic animals. Res Vet Sci, 47, 34-44. Eichhorn M, Flugel C (1988). Histochemical demonstration of carbonic anhydrase and Na/K-ATPase in the pecten oculi of the fowl. Exp Eye Res, 47, 147-153. Gerhardt H, Liebner S, Wolburg H (1996). The pecten oculi of the chicken as a new in vivo model of the blood-brain barrier. Cell Tissue Res, 285, 91-100. Karnovsky MJ (1965). Formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol, 27, 137A-138A. Kiama SG, Bhattacharjee T, Maina JN, Weyrauch KD (1994). A scanning electron microscope study of the pecten oculi of the black kite: possible involvement of melanosomes in protecting the pecten against damage by ultraviolet light. J Anat, 185, 637-642. Kiama SG, Bhattacharjee T, Maina JN., Weyrauch KD (1997). Surface specialization of the capillary endothelium in the pecten oculi of the chicken and their overt roles in pectineal hemodynamics and nutrient transfer to the inner neuronal retina. Acta Biol Hung, 48, 473-483. Kiama SG, Maina JN, Bhattacharjee T, Weyrauch KD, Gehr P (1998). A scanning electron microscope study of the luminal surface specializations in the blood vessels of the pecten oculi in a diurnal bird, the black kite. Anat Anz, 180, 455-460. Kiama SG, Maina JN, Bhattacharjee T, Mwangi DK, Macharia RG, Weyrauch KD (2009). The morphology of the pecten oculi of the ostrich, Struthio camelus. Annals Anatomy, 188, 519-528. Korkmaz D, Kum S (2016): Investigation of the antigen recognition and presentation capacity of pecteneal hyalocytes in the chicken (Gallus gallus domesticus). Biotech & Histochem, 91(3), 212-219. Liebner S, Gerhardt H, Wolburg H (1997). Maturation of the blood-retina barrier in the developing pecten oculi of the chicken. Dev Brain Res, 100, 205-219. Llombart C, Nacher V, Ramos D, Luppo M, Carretero A, Navarro M, Melgarejo V, Armengol C, Rodriquez-Baeza A, Mendes-Jorge L, Ruberte J (2009). Morphological characterization of pecteneal hyalocytes in the developing quail retina. J Anat, 215, 280-291. Nickel R, Schummer A, Seiferle E (1977). Anatomy of the Domestic Birds. Verlag Paul Parey Berlin/Hamburg p: 153-154. Orhan OI, Ekim O, Bayraktaroğlu AG (2011). Morphological investigation of the pecten oculi in quail (Coturnix coturnix japonica). Vet J Ankara Univ, 58, 5-10. Rahman ML, Lee E, Aoyama M, Sugita S (2010). Light and electron microscopy study of the pecten oculi of the jungle crow (Corvus macrorhynchos). Okajimas Folia Anat Jpn, 87, 75-83 Smith BJ, Smith SA, Braekevelt CR (1996). Fine structure of the pecten oculi of the Barred owl. Histol Histopathol, 11(1), 89-96. Tucker R (1975). The surface of the pecten oculi in the pigeon. Cell and Tissue Research, 157 (4), 457-465. Uhera M, Imagawa T, Kitagawa H (1996). Morphological studies of the hyalocytes in the chicken eye: scanning electron microscopy and inflammatory response after the intra vitreous injection of carbon particles. J Anat, 188, 661-669. Veneable J, Coggeshall R (1965). A simplified lead citrate stain for use in electron microscopy. J Cell Biol, 45, 407-408. Wolburg H, Liebner S, Reichenbach A, Gerhardt H (1999). The pecten oculi of the chicken: a model system for vascular differentiation and barrier maturation. Int Rev Cytol, 187, 11-159.
Abstract
Yapılan bu araştırmada devekuşu pekten okulisinin ışık ve elektron
mikroskobik olarak incelenmesi amaçlandı. Çalışmada et tüketimine sunulan ve mezbahanede
kesilen 5 adet erişkin ve sağlıklı devekuşundan alınan pekten okuli örnekleri
kullanıldı. Makroskobik incelemeler sonucu, devekuşunda pekten okulinin gözün
ventral yüzeyinden vitreusa doğru uzanan, koyu pigmentli, kendi üzerinde
yaklaşık 20-24 adet kıvrım yapan, oldukça büyük bir yapı olduğu görüldü. Işık
mikroskobik incelemelerde pekten okulinin her bir kıvrımında çok sayıda
kapillar ve melanosit yanında az sayıda arter ve ven de bulunmaktaydı.
Melanositlerin pekten kıvrımlarının perifer kısmında, damarların etrafında
yoğunlaştığı görüldü. Yapının etrafını pekteno-sınırlayıcı membran
çevrelemekteydi. Devekuşu pekten okulisinde hyalositlere rastlanmadı. TEM’de
(Transmission Electron Microscope) endotel hücrelerinin ince stoplazmaya sahip
olduğu ve organellerin hücrenin merkezinde, çekirdek etrafında yer aldığı
gözlendi. Kapillar endotelinin çok sayıda luminal ve abluminal kıvrımı
bulunmaktaydı ve kalın bir bazal laminaya sahipti. Bu laminanın altında ise
perisiler gözlendi. Sunulan çalışmada devekuşu pekten okulisi ışık ve elektron
mikroskobik olarak incelendi ve diğer kanatlı türleriyle karşılaştırılarak
farklılıkları ortaya kondu.
Keywords
References
- Amemiya T (1985). Constituents of connective tissue around the capillary of chick pecten oculi. Acta Anat, 122, 235-238. Braekevelt CR (1984). Electron microscopic observation on the pecten oculi of the nighthawk. Ophthalmologica, 189, 211-220. Braekevelt CR (1988). Fine structure of the pecten oculi of the pigeon. Ophthalmologica, 196, 151-159. Braekevelt CR (1991). Electron microscopic observation on the pecten oculi of the gread blue heron. Histol Histopathol, 6, 345-351. Braekevelt CR (1991a). Fine structure of the pecten oculi in the red-tailed hawk. Anat Histol Embryol, 8, 9-15. Braekevelt CR (1993). Fine structure of the pecten oculi in the great horned owl. Histol Histopathol, 8, 9-15. Braekevelt CR (1994). Fine structure of the pecten oculi in the American crow. Anat. Histol Embryol, 23, 357-366. Braekevelt CR (1998). Fine structure of the pecten oculi of the emu. Tissue Cell, 30, 157-165. Dayan MO, Ozaydin TA (2013). Comparative morphomertical study of the pecten oculi in different avian species. Sci World J, Article ID: 968652 Denk H, Kunzele H, Plenk H, Ruschoff J, Sellner W (1989). Romeis Microscopische Tecnic, 17, Neubearbeitete Auflage. Urban und Schwarzenberg, München, Wien, Baltimore, p: 439-450. De Schaepdrijver L, Simoens P, Lauwers H, De Geest JP (1989). Retinal vascular patterns in domestic animals. Res Vet Sci, 47, 34-44. Eichhorn M, Flugel C (1988). Histochemical demonstration of carbonic anhydrase and Na/K-ATPase in the pecten oculi of the fowl. Exp Eye Res, 47, 147-153. Gerhardt H, Liebner S, Wolburg H (1996). The pecten oculi of the chicken as a new in vivo model of the blood-brain barrier. Cell Tissue Res, 285, 91-100. Karnovsky MJ (1965). Formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol, 27, 137A-138A. Kiama SG, Bhattacharjee T, Maina JN, Weyrauch KD (1994). A scanning electron microscope study of the pecten oculi of the black kite: possible involvement of melanosomes in protecting the pecten against damage by ultraviolet light. J Anat, 185, 637-642. Kiama SG, Bhattacharjee T, Maina JN., Weyrauch KD (1997). Surface specialization of the capillary endothelium in the pecten oculi of the chicken and their overt roles in pectineal hemodynamics and nutrient transfer to the inner neuronal retina. Acta Biol Hung, 48, 473-483. Kiama SG, Maina JN, Bhattacharjee T, Weyrauch KD, Gehr P (1998). A scanning electron microscope study of the luminal surface specializations in the blood vessels of the pecten oculi in a diurnal bird, the black kite. Anat Anz, 180, 455-460. Kiama SG, Maina JN, Bhattacharjee T, Mwangi DK, Macharia RG, Weyrauch KD (2009). The morphology of the pecten oculi of the ostrich, Struthio camelus. Annals Anatomy, 188, 519-528. Korkmaz D, Kum S (2016): Investigation of the antigen recognition and presentation capacity of pecteneal hyalocytes in the chicken (Gallus gallus domesticus). Biotech & Histochem, 91(3), 212-219. Liebner S, Gerhardt H, Wolburg H (1997). Maturation of the blood-retina barrier in the developing pecten oculi of the chicken. Dev Brain Res, 100, 205-219. Llombart C, Nacher V, Ramos D, Luppo M, Carretero A, Navarro M, Melgarejo V, Armengol C, Rodriquez-Baeza A, Mendes-Jorge L, Ruberte J (2009). Morphological characterization of pecteneal hyalocytes in the developing quail retina. J Anat, 215, 280-291. Nickel R, Schummer A, Seiferle E (1977). Anatomy of the Domestic Birds. Verlag Paul Parey Berlin/Hamburg p: 153-154. Orhan OI, Ekim O, Bayraktaroğlu AG (2011). Morphological investigation of the pecten oculi in quail (Coturnix coturnix japonica). Vet J Ankara Univ, 58, 5-10. Rahman ML, Lee E, Aoyama M, Sugita S (2010). Light and electron microscopy study of the pecten oculi of the jungle crow (Corvus macrorhynchos). Okajimas Folia Anat Jpn, 87, 75-83 Smith BJ, Smith SA, Braekevelt CR (1996). Fine structure of the pecten oculi of the Barred owl. Histol Histopathol, 11(1), 89-96. Tucker R (1975). The surface of the pecten oculi in the pigeon. Cell and Tissue Research, 157 (4), 457-465. Uhera M, Imagawa T, Kitagawa H (1996). Morphological studies of the hyalocytes in the chicken eye: scanning electron microscopy and inflammatory response after the intra vitreous injection of carbon particles. J Anat, 188, 661-669. Veneable J, Coggeshall R (1965). A simplified lead citrate stain for use in electron microscopy. J Cell Biol, 45, 407-408. Wolburg H, Liebner S, Reichenbach A, Gerhardt H (1999). The pecten oculi of the chicken: a model system for vascular differentiation and barrier maturation. Int Rev Cytol, 187, 11-159.
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | August 20, 2017 |
| Submission Date | June 2, 2017 |
| Acceptance Date | September 5, 2017 |
| Published in Issue | Year 2017 Volume: 28 Issue: 2 |
Cite
Accepted papers are licensed under Creative Commons Attribution-NonCommercial 4.0 International License