Nitrate and Nitrite Levels in Serum and Seminal Plasma in Horses

Year 2018, Volume: 29 Issue: 3, 159 - 162, 27.12.2018

Leyla Mis , Handan Mert İnci Söğütlü Nihat Mert , Kıvanç Irak

Abstract

Nowadays, the fertility of the growing horses is of great importance. The aim of our study is to determine and compare the level of nitric oxide oxidation products of these animals in serum and seminal plasma. Seven healthy British horses were used in the study. Blood samples were taken from the vena jugularis. The sera were removed by centrifugation. Seminal plasmas of ejaculates taken with artificial vagina were separated. Seminal plasma and serum were obtained and nitrate nitrite values were determined. Nitrate and nitrite levels of samples were measured by coupling reagent reagent method. Nitrate levels in both semis and seminal plasma were 6,34 ± 2,7 and 4,95 ± 1,4, respectively. Nitrite levels were higher than seminal plasma in seminal plasma (p≤ 0.05), while nitrite levels in seminal plasma were higher than serum (p≤ 0.05). As a result, seminal plasma and serum nitrate-nitrite values ​​in British stallions were used. Presented to the knowledge of clinical veterinary medicine.

Keywords

Horse, Nitrate, Nitrite, Seminal plasma

Atlarda Serum ve Seminal Plazmada Nitrat ve Nitrit Düzeyleri

Abstract

Günümüzde yetiştiriciliği giderek artan atların fertilitesinin önemi büyüktür. Çalışmamızın amacı, bu hayvanların serum ve seminal plazmada nitrik oksit oksidasyon ürünlerinin seviyesini belirleyip karşılaştırmaktır.Çalışmada 7 adet sağlıklı İngiliz atı kullanıldı. Kan örnekleri vena jugularisten usulüne uygun alındı. Santrifüj edilerek serumlar çıkarıldı. Suni vajen yardımı ile alınan ejakulatlarının seminal plazmaları ayrıldı.  Seminal plazma ve serum elde edilerek nitrat nitrit değerleri belirlendi. Alınan örneklerde nitrat ve nitrit düzeyleri coupling reaktif ayıracı yöntemi ile ölçüldü. Serum ve seminal plazmadaNitrat düzeyleri sırasıyla 6,34±2,7 ve 4,95 ±1,4 iken Nitrit 1,55±0,7 ve 2,02±0,45 olarak belirlendi. Serumda nitrat seminal plazmadan yüksek (p≤ 0.05) iken seminal plazmada nitrit düzeyi serumdan daha yüksek (p≤ 0.05) hesaplandı. Sonuç olarak İngiliz aygırlarında seminal plazma ve serum nitrat-nitrit değerleri kaliteli sperma hakkında fikir sahibi olmak için klinik veteriner hekimliğin bilgisine sunuldu.

Keywords

At, Nitrat, Nitrit, Seminal plazma

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