Investigation of the Antidiabetic Effects of Mistletoe (Viscum album L.) Extract in Experimental Diabetes in Rats

Year 2019, Volume: 30 Issue: 2, 121 - 125, 26.07.2019

Avin Kawa Ahmed Nihat Mert , Handan Mert

Abstract

Diyabet çoklu etiyolojisi, karbonhidrat, yağ ve protein metabolizması
bozuklukları ve kronik hiperglisemi ile seyreden metabolik bir hastalıktır. V. Album (ökse otu) yaprak ekstreleri,
diyabet semptomlarını hafifletmek, diyabete yararlı bir etki sağlamak için
kullanılan yöresel bir bitkidir. Bu çalışmanın amacı, V. album ekstraktlarının streptozotosin (STZ) kaynaklı diyabetik
sıçanlar üzerindeki antidiyabetik ve antioksidan aktivitelerini araştırmaktır. Araştırmada
32 adet, dişi 6-7 haftalık Wistar sıçanları kullanıldı. Sıçanlar rastgele 4
gruba, ayrıldı. Grup I  (Kontrol), Grup II (V.
album), Grup III (Diyabetik), Grup IV (Diyabetik + V. album). Deneme sonunda tüm gruplardaki sıçanların kan örnekleri
alınıp hemen serumları ayrıldı. Total
kolesterol, HDL, trigliserit, folik asit, vitamin D, vitamin B₁₂  ve glukoz düzeyleri otoanalizörde saptandı.
TAC ve TOS analizleri ELISA’da ölçüldü. Diyabetik+V. album grubunda diyabet
grubuna göre glukoz (p≤0.05), vitamin B₁₂ (p≤0.05)  ve TOS (p≤0.05)  düzeyleri önemle azalırken, TAC düzeyi önemle
yükseldi (p≤0.05). Sonuç olarak; V.
album ekstreleri  antidiyabetik
etkilere sahiptir. Sadece 20 günlük deneme süresince V. album ile tedavi edildiğinde glukoz seviyesini önemli ölçüde
azaltmıştır. V. album, diyabetik
komplikasyonların oluşumunu önlemeye yardımcı olabilecek güçlü bir antioksidan
aktiviteye sahiptir.

Keywords

Diyabet, Lipoproteinler, Ökse otu, Toplam antioksidan kapasite, Toplam oksidatif durum

Supporting Institution

Van YYU Directory of Scientific Research

Project Number

2016- TYL-5198

References

• REFERENCES Aschner P, Aguilar-Salinas C, Aguirre L, Franco L, Gagliardino JJ, de Lapertosa SG, Seclen, S, Vinocour M (2014). Diabetes in South and Central America, an update. Diabetes Research and Clinical Practice, 103 (2), 238-243. Aslan R, Kutlu R, Civi S, Tasyurek E (2014). The correlation of the total antioxidant status (TAS), total oxidant status (TOS) and paraoxonase activity (PON1) with smoking. Clinical Biochemistry, 47(6), 393-397. Audelin MC, Genest J (2001). Homocysteine and cardiovascular disease in diabetes mellitus. Atherosclerosis, 159 (2), 497-511. Bailey LB, MolLoy A, Scott J, Rice D (1989). Streptozotocin-induced diabetes is not a model for methylmalonic acidaemia. Journal of Inherited Metabolic Disease, 12(4), 429-435. Bhatt HR, Linnell JC (1983) Induction of ethylmalonic acidaemia with streptozotocin: a possible experimental model for the study of methylmalonic acidaemia. Journal of Inherited Metabolic Disease, 6, Suppl, 2. Bhatt HR, Linnell, JC, Matthews DM (1983). Can faulty vitamin B12 (Cobalamin) metabolism produce diabetic neuropathy. The Lancet, 3, 572. Barbosa CF, Tonin AA, Da Silva AS, De Azevedo MI, Monteiro DU, Waczuk EP, Faccio L (2014). Diphenyl diselenide and sodium selenite associated with chemotherapy in experimental toxoplasmosis: influence on oxidant/antioxidant biomarkers and cytokine modulation. Parasitology, 141 (13) 1761-1768. Bartosz G, Bartosz M (1998). Antioxidant activity: What do we measure? Acta Biochimica Polonica, 46 (1), 23-29. Baytop T (1984). Therapy with Medicinal Plants in Turkey (Past and Present), Publications of IX Istanbul University, 243. Davidson MB, Duran P, Lee ML, Friedman TC (2013). High-dose vitamin D supplementation in people with prediabetes and hypovitaminosis. Diabetes Care, 36 (2), 260-266. De Silva DD, Rapior S, Hyde KD, Bahkali AH (2012). Medicinal mushrooms in prevention and control of diabetes mellitus. Fungal Diversity, 56 (1), 1-29. Erel O (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38, 1103-1111. Fraga CG, Oteiza PI, Galleano M (2014). In vitro measurements and interpretation of total antioxidant capacity. Biochemical and Biophysical Acta, 1840 (2), 931-934. Giacco F, Brownlee M (2010). Oxidative stress and diabetic complications. Circulation Research, 107(9), 1058-1070. Gray AM, Flatt PR (1999). Insulin-secreting activity of the traditional antidiabetic plant Viscum album (mistletoe). Journal of Endocrinology, 160 (3), 409-414. Gupta MM, Chari S (2005). Lipid peroxidation and antioxidant status in patients with diabetic retinopathy. Indian Journal of Physiology & Pharmacology, 49 (2), 187. Hunt JV, Smith CC, Wolff SP (1990). Autoxidative glycosylation and possible involvement of peroxides and free radicals in LDL modification by glucose. Diabetes, 39(11), 1420-1424. International Diabetes Federation (2015). Diabetes Atlas: Update of mortality attributable to diabetes for the IDF Diabetes Atlas: Estimates for the year 2013. Diabetes Research and Clinical Practice, 109 (3), 461-465. Jung M, Park M, Lee HC, Kang YH, Kang ES, Kim SK, (2006). Antidiabetic agents from medicinal plants. Current Medicinal Chemistry, 13, 10, 1203-1218. Kakkar R, Mantha SV, Radhi J, Prasad K, Kalra, J (1998). Increased oxidative stress in rat liver and pancreas during progression of streptozotocin-induced diabetes. Clinical Science, 94, 623-632. Kaneto H, Katakami N, Kawamori D, Miyatsuka T, Sakamoto KY, Matsuoka TA, Yamasaki Y (2007). Involvement of oxidative stress in the pathogenesis of diabetes. Antioxidants Redox Signaling, 9(3), 355-366. Karabay G, Zağyapan R, Take G (2006). An ultrastructural study of effects of streptozotocin induced diabetes on rats peripheral nerves. Journal of Uludağ University Medical Faculty, 32 (3), 77-81. Kollias AN, Ulbig MW (2010). Diabetic retinopathy: early diagnosis and effective treatment. Deutsches Arzteblatt International, 107(5), 75-83. Kuzuya T, Nakagawa S, Satoh J, Kanazawa Y, Iwamoto Y, Kobayashi M, Shima K (2002). Report of the Committee on the classification and diagnostic criteria of diabetes mellitus. Diabetes Research Clinical Practice, 55 (1), 65-85. Liu KW, Dai LK, Jean W (2006). Metformin-related vitamin B12 deficiency. Age and Ageing, 35( 2), 200-201. Malviya N, Jain S, Malviya SAP (2010). Antidiabetic potential of medicinal plants. Acta Poloniae Pharmaceutica, 67(2), 113-118. Miller AG (1982). Viscum album L. Flora of Turkey and the East Aegean Islands. Edinburgh University Press, 7, 547. Orhan DD, Aslan M, Sendogdu N, Ergun F, Yesilada E (2005). Evaluation of the hypoglycemic effect and antioxidant activity of three Viscum album subspecies in streptozotocin-diabetic rats. J ethnopharm, 98 (1), 95-102. Öztabak KÖ (2005). The Anti-Carcinogen Activities of Lectins and Viscum Album Agglutinin (VAA). Journal of Faculty Veterinary Medicine Erciyes University, 2, 55-59. Simmons RA (2006). Developmental origins of diabetes: the role of oxidative stress. Free Radical Biology & Medicine, 40(6), 917-922. Simsek I, Aytekin F, Yesilada E, Yildirimli S (2004). An ethnobotanical survey of the Beypazari, Ayas, and Gudul district towns of Ankara Province (Turkey). Economic Botany, 58, 4. Sone H, Tanaka S, Tanaka S, Iimuro S, Ishibashi S, Oikawa S, Yamada N (2012). Comparison of various lipid variables as predictors of coronary heart disease in japanese men and women with type 2 diabetes sub analysis of the Japan diabetes complications study. Diabetes Care, 35 (5), 1150-1157. Swanston-Flatt SK, Day C, Bailey, CJ, Flatt PR (1989). Evaluation of traditional plant treatments for diabetes: studies in streptozotocin diabetic mice. Acta Diabetologia Latina, 26(1), 51-55. Tiwari BK, Pandey KB, Abidi AB, Rizvi SI (2013). Markers of oxidative stress during diabetes mellitus. Journal of Biomarkers, 10, 1155, 8-17. Uchigata Y, Yamamoto H, Kawamura A, & Okamoto H (1982). Protection by superoxide dismutase, catalase, and poly synthetase inhibitors against alloxan-and streptozotocin-induced islet DNA strand breaks and against the inhibition of proinsulin synthesis. The Journal of Biological Chemistry, 257(11) 6084-6088. Weiss RB (1982). Streptozotocin: a review of it pharmacology, efficacy and toxicity. Cancer Treatment Reports, 66, 427-438. Widatalla A, Mahadi SE, Shawer M, Elsayem H, Ahmed M (2009). Implementation of diabetic foot ulcer classification system for research purposes to predict lower extremity amputation. International Journal of Diabetes in Developing Countries, 29 (1), 1. World Health Organisation (2014). About diabetes. World Health Organization: Retrieved April 4, 2014. Zeitz U, Weber K, Soegiarto DW, Wolf E, Balling R, Erben, RG (2003). Impaired insulin secretory capacity in mice lacking a functional vitamin D receptor. FASEB Journal, 17 (3), 509-511.