Farklı Stres Uygulamalarının Bazı Üreme Hormonları, Sperm Parametreleri, Lipid Profili, İmmünohistokimyasal ve İmmünofloresan Belirteçler Üzerine Etkileri

Year 2023, Volume: 34 Issue: 3, 263 - 270, 27.11.2023

Saadet Belhan , Zübeyir Huyut , Serkan Yildirim , Sermin Algül

https://doi.org/10.36483/vanvetj.1326578

Abstract

Mevcut çalışma, uygulanan 5 stres protokolünün sperm parametrelerini, lipid profilini ve bazı üreme hormonlarını nasıl etkilediğini değerlendirdi. Çalışmanın canlı materyalini 50 sıçan oluşturdu. Gruplardaki sıçan sayısı eşitti ve rastgele dağıtıldılar. Her grup 10 sıçandan oluşuyordu. Kontrol grubuna herhangi bir stres uygulaması yapılmadı. Psikolojik stres grubundaki sıçanlar günde 4 saat aydınlık, 20 saat karanlık döngüsüne tabi tutuldu. Fiziksel stres grubundaki sıçanlar günde 2 saat yem ve sudan mahrum bırakıldı. Psikolojik stres + fiziksel stres grubunda psikolojik ve fiziksel stres protokolü uygulandı. İlk 4 grupta tüm uygulamalar 14 gün süreyle yapılmıştır. Depresyon grubundaki sıçanlara her gün farklı bir stres uygulama uygulandı. Anormal sperm oranının stres ve depresyon gruplarında yüksek olduğu ancak en yüksek oranın depresyon grubunda olduğu belirlendi. Ayrıca sperm motilitesi ve yoğunluğu depresyon grubunda en düşüktü. Stres ve depresyon gruplarında serum trigliserit ve HDL düzeyleri ile LH ve FSH düzeyleri anlamlı olarak düşük bulunurken, kolesterol ve LDL değerleri anlamlı olarak yüksekti. Bax ekspresyonu ve 8 OHdG ekspresyonu psikolojik stress + fiziksel stres grubu ve depresyon grubunda şiddetli düzeydeydi. Bulgular toplu olarak değerlendirildiğinde; stresin sperm parametrelerini, lipid profilini, üreme hormonlarını, immünofloresan ve immünohistokimyasal parametreleri olumsuz etkilediği belirlendi.

Keywords

Bax, LDL, LH, Sperm, Stres, Trigliserit

Effects of Different Stress Applications on Some Reproductive Hormones, Sperm Parameters, Lipid Profile, Immunohistochemical and Immunofluorescent Markers

Abstract

The current study assessed how 5 stress protocols applied affected sperm parameters, lipid profile, and some reproductive hormones. Live materials of the study consisted of 50 rats. The number of rats in the groups was equal and they were randomly assigned. Each group consisted of 10 rats. No stress application was conducted in the control group. The rats in the psychological stress group were subjected to a cycle of 4-hour light and 20-hour dark per day. The rats in the physical stress group were deprived of feed and water for two hours per day. In the psychological stress + physical stress group, the psychological and physical stress protocol was conducted. In the first 4 groups, all the applications were conducted for 14 days. A different stress application was applied to the rats in the depression group every day. It was determined that the abnormal sperm rate was high in the stress and depression groups, but the highest rate was in the depression group. In addition, sperm motility and sperm concentration were the lowest in the depression group. While the stress and depression groups had significantly lower serum triglyceride and HDL levels and LH and FSH levels, cholesterol and LDL values were significantly higher. Bax expression and 8 OHdG expression were severe in psychological stress+physical stress group and depression group. When the findings are evaluated collectively; it was determined that stress negatively affected sperm parameters, lipid profile, reproductive hormones, immunofluorescence and immunohistochemical parameters.

Keywords

Bax, LDL, LH, Sperm, Stress, Triglyceride

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