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Preovulatör folikülün vaskülarizasyonu: Arap kısraklarda erken gebelik sonuçlarında renkli Doppler değerlendirmesi ve öngörülen değeri

Year 2020, Volume: 91 Issue: 2, 104 - 109, 15.06.2020
https://doi.org/10.33188/vetheder.672255

Abstract

Sunulan makalede renkli Doppler ultrasonografi ile preovulatör folikül çeperindeki damarlaşma düzeyinin gebelik oluşumuyla ilişkisinin belirlenmesi amaçlandı. Üreme sezonundaki sağlıklı 26 adet Arap kısraktan alınan renkli Doppler ultrasonografi görüntüleri değerlendirildi. Sakin mizaçlı ve reprodüktif açıdan problemi olmayan kısraklar seçildi. Preovulatör folikül (>35mm), transrektal B-mod ultrasonografi ve renkli Doppler ultrasonografi ile ovulasyon gününe dek günde iki kere izlendi. Çalışmaya dahil edilen foliküler vaskülarizasyon görüntüleri yumurtlamadan 18 saat önce izlendi. Ayrıca, renkli Doppler üzerinden akım görülen alanlardaki piksel sayısı bilgisayar destekli görüntü analiz programı ile değerlendirildi. Kısraklar, bir aygırla doğal aşım yoluyla çiftleştirildi. Aşımı izleyen 14-30 günlerde ultrasonografi ile gebelik muayeneleri yapıldı. Bunun neticesinde kısraklar gebe (n=13) ve gebe olmayan (n=13) olmak üzere iki gruba ayrıldı. Gebe kısraklara ait preovulatör folikül çeperindeki damarlaşma miktarı ile gebe olmayan kısraklara ait preovulatör folikül çeperindeki damarlaşma miktarı arasındaki istatistiksel fark t-testi ile karşılaştırıldı. Çalışmanın sonucunda, preovulatör folikül çeperindeki renkli piksellere ait alan, hacim ve yoğunluk düzeylerinin gebe ve gebe olmayan kısraklarda farklı olmadığı görüldü (P>0,05). Sonuç olarak, üreme sezonundaki kısraklarda preovulatör follikül duvarına ait renkli Doppler görüntülerinin kantitatif değerlendirmesinin gebelik tanısında kullanılamayacağı görüldü.

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References

  • Acosta TJ, Beg MA, Ginther OJ (2004): Aberrant blood flow area and plasma gonadotropin concentrations during the development of dominant-sized transitional anovulatory follicles in mares. Biol Reprod, 71, 637–642.
  • Altermatt JL, Marolf AJ, Wrigley RH, Carnevale EM (2012): Effects of FSH and LH on ovarian and follicular blood flow, follicular growth and oocyte developmental competence in young and old mares. Anim Reprod Sci, 133, 191– 197.
  • Bekyürek T, Canoğlu C, Demiral O, Ün M, Abay M (2012): Kısraklarda östrusun uyarılmasında prid kullanımı. J Fac Vet Med Univ Erciyes, 9(1), 29-32.
  • Cengiz M, Çolak A, Polat B, Chacher MFA (2018): Ultrasonographic B-mod echotexture analysis of genital organs in veterinary gynecology. Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics, 4(1), 55-61.
  • Erdoğan G (2018): Using of Doppler ultrasonography in veterinary gnecology. Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics, 4(1),43-49.
  • Ginther OJ, Gastal EL, Gastal MO, Siddiqui MA, Beg MA (2007): Relationships of follicle versus oocyte maturity to ultrasound morphology, blood flow, and hormone concentrations of the preovulatory follicle in mares. Biol Reprod, 77, 202–208.
  • Ginther OJ (2008): How ultrasound technologies have expanded and revolutionized research in reproduction in large animals. Theriogenology, 81, 112–125.
  • Ginther OJ, Rakesh HB, Hoffman MM (2014): Blood flow to follicles and CL during development of the periovulatory follicular wave in heifers. Theriogenology, 82, 304-311.
  • Kılıçarslan MR, Uçar M (2015): Genital organların muayenesi. 45-66. In: M Kaymaz, M Fındık, A Rişvanlı, A Köker (Eds), Kısraklarda Doğum ve Jinekoloji. Medipres, Malatya.
  • Kılıçarslan MR, Tek Ç, Sabuncu A, Uçar M (2018): Gynecological transrektal ultrasonography for equine breeding. Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics, 4(1), 16-20.
  • Mortensen CJ, Kelly DE, Smith RL, Adkin A (2012): Predicting a fertile cycle: studies examining vascular perfusion of the preovulatory follicle via Doppler ultrasonography. J Equine Vet Sci, 32, 397-422.
  • Siddiqui MAR, Almamun M, Ginther OJ (2009): Blood flow in the wall of the preovulatory follicle and its relationship to pregnancy establishment in heifers. Anim Reprod Sci, 113, 287-92.
  • Silva LA, Gastal EL, Gastal MO, Beg MA, Ginther OJ (2006): Relationship between vascularity of the preovulatory follicle and establishment of pregnancy in mares. Anim Reprod, 3, 339-346.
  • Şenünver A, Gültiken N (2015): Gebelik ve fizyolojisi. 97-113. In: Kaymaz M, Fındık M, Rişvanlı A, Köker A, (Eds.), Kısraklarda Doğum ve Jinekoloji, Medipres, Malatya.
  • Varughese EE, Brar PS, Ghuman SS (2017): Vascularization to preovulatory follicle and corpus luteum-a valuable predictor of fertility in dairy cows. Theriogenology, 103, 59-68.

The vascularity of preovulatory follicle: The colour–Doppler assessment and its predictive value in the early pregnancy outcome in Arabian Mares

Year 2020, Volume: 91 Issue: 2, 104 - 109, 15.06.2020
https://doi.org/10.33188/vetheder.672255

Abstract

The aim of this study is to determine the relationship between the amount of vascularization in the preovulatory follicle wall and pregnancy establisment with colour Doppler ultrasonography. Colour Doppler ultrasonography images from 26 Arabian mares in breeding season were evaluated in the study. Mares no abnormalities in the reproductive system and mild-manner mares were handled. Preovulatory follicle (>35mm) was monitored twice in a day by transrectal B-mode ultrasonography and colour Doppler ultrasonography until the ovulation day. Follicular vascularization images which were incorporated into the study, were monitored 18 hours before the ovulatio Also amount of pixels in colour Doppler images were evaluated with computer-based image analysis program. The mares were mated naturally with a stallion. Pregnancy diagnosis was performed by ultrasonography on day 14 to day 30 after mating. As a result of ultrasonography examination, mares were divided into two groups as pregnant (n=13) and non-pregnant (n=13). The statistical difference between the amount of vascularization in the preovulatory follicle wall of pregnant mares and the amount of vascularization in the preovulatory follicle wall of non-pregnant mares was compared by t-test. As a result of the study, there were no significant differences between pregnant and non-pregnant mares in terms of area, volume and intensity units of coloured pixels in the preovulatory follicle wall (P> 0.05). In conclusion, it was observed that the quantitative evaluation of the colour Doppler images of the preovulatory follicle wall in mares in the breeding season cannot be used in the diagnosis of pregnancy.

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Project Number

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Thanks

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References

  • Acosta TJ, Beg MA, Ginther OJ (2004): Aberrant blood flow area and plasma gonadotropin concentrations during the development of dominant-sized transitional anovulatory follicles in mares. Biol Reprod, 71, 637–642.
  • Altermatt JL, Marolf AJ, Wrigley RH, Carnevale EM (2012): Effects of FSH and LH on ovarian and follicular blood flow, follicular growth and oocyte developmental competence in young and old mares. Anim Reprod Sci, 133, 191– 197.
  • Bekyürek T, Canoğlu C, Demiral O, Ün M, Abay M (2012): Kısraklarda östrusun uyarılmasında prid kullanımı. J Fac Vet Med Univ Erciyes, 9(1), 29-32.
  • Cengiz M, Çolak A, Polat B, Chacher MFA (2018): Ultrasonographic B-mod echotexture analysis of genital organs in veterinary gynecology. Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics, 4(1), 55-61.
  • Erdoğan G (2018): Using of Doppler ultrasonography in veterinary gnecology. Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics, 4(1),43-49.
  • Ginther OJ, Gastal EL, Gastal MO, Siddiqui MA, Beg MA (2007): Relationships of follicle versus oocyte maturity to ultrasound morphology, blood flow, and hormone concentrations of the preovulatory follicle in mares. Biol Reprod, 77, 202–208.
  • Ginther OJ (2008): How ultrasound technologies have expanded and revolutionized research in reproduction in large animals. Theriogenology, 81, 112–125.
  • Ginther OJ, Rakesh HB, Hoffman MM (2014): Blood flow to follicles and CL during development of the periovulatory follicular wave in heifers. Theriogenology, 82, 304-311.
  • Kılıçarslan MR, Uçar M (2015): Genital organların muayenesi. 45-66. In: M Kaymaz, M Fındık, A Rişvanlı, A Köker (Eds), Kısraklarda Doğum ve Jinekoloji. Medipres, Malatya.
  • Kılıçarslan MR, Tek Ç, Sabuncu A, Uçar M (2018): Gynecological transrektal ultrasonography for equine breeding. Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics, 4(1), 16-20.
  • Mortensen CJ, Kelly DE, Smith RL, Adkin A (2012): Predicting a fertile cycle: studies examining vascular perfusion of the preovulatory follicle via Doppler ultrasonography. J Equine Vet Sci, 32, 397-422.
  • Siddiqui MAR, Almamun M, Ginther OJ (2009): Blood flow in the wall of the preovulatory follicle and its relationship to pregnancy establishment in heifers. Anim Reprod Sci, 113, 287-92.
  • Silva LA, Gastal EL, Gastal MO, Beg MA, Ginther OJ (2006): Relationship between vascularity of the preovulatory follicle and establishment of pregnancy in mares. Anim Reprod, 3, 339-346.
  • Şenünver A, Gültiken N (2015): Gebelik ve fizyolojisi. 97-113. In: Kaymaz M, Fındık M, Rişvanlı A, Köker A, (Eds.), Kısraklarda Doğum ve Jinekoloji, Medipres, Malatya.
  • Varughese EE, Brar PS, Ghuman SS (2017): Vascularization to preovulatory follicle and corpus luteum-a valuable predictor of fertility in dairy cows. Theriogenology, 103, 59-68.
There are 15 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Zeynep Günay Uçmak 0000-0003-2530-1291

İbrahim Kurban 0000-0002-8391-905X

Melih Uçmak 0000-0002-8688-127X

Project Number -
Publication Date June 15, 2020
Submission Date January 9, 2020
Acceptance Date April 25, 2020
Published in Issue Year 2020 Volume: 91 Issue: 2

Cite

Vancouver Günay Uçmak Z, Kurban İ, Uçmak M. The vascularity of preovulatory follicle: The colour–Doppler assessment and its predictive value in the early pregnancy outcome in Arabian Mares. Vet Hekim Der Derg. 2020;91(2):104-9.

Veteriner Hekimler Derneği Dergisi (Journal of Turkish Veterinary Medical Society) is an open access publication, and the journal’s publication model is based on Budapest Access Initiative (BOAI) declaration. All published content is licensed under a Creative Commons CC BY-NC 4.0 license, available online and free of charge. Authors retain the copyright of their published work in Veteriner Hekimler Derneği Dergisi (Journal of Turkish Veterinary Medical Society). 

Veteriner Hekimler Derneği / Turkish Veterinary Medical Society