Evaluation of the variations in spermatological parameters of Arabian stallions according to different periods of breeding season

Year 2024, Volume: 95 Issue: 1, 1 - 9, 15.01.2024

Kemal Tuna Olğaç , Mehmet Borga Tırpan

https://doi.org/10.33188/vetheder.1353901

Abstract

In this study, determining of the variations on spermatological characteristics of Arabian stallion semen in the different periods of the breeding season is aimed. The periods have been described as the beginning of the season (BS: 15th February – 15th March), mid-season (MS: 15th April – 15th May), and end of the season (ES: 30th May – 30th June). A total of 7 Arabian stallions that were 5-10 years old, with body condition scores between 3-3.5 and without any problems in terms of general and reproductive health, were enrolled in the study. In total, three semen samples were collected from each stallion, once in each period. Then, these samples were evaluated in terms of volume, concentration, total motility (TMOT), progressive motility (PMOT), viability, high mitochondrial membrane potential (HMMP), acrosome integrity (AI), capacitation index (Ci), and lipid peroxidation (LPO) parameters, which were analyzed by computer assisted sperm analysis and flowcytometry devices. As a result, the differences in volume, concentration, TMOT, and PMOT between the BS, MS, and ES periods were statistically insignificant (p>0.05). The lowest viability values were determined in the BS period (p<0.001). The highest values of the HMMP were obtained in the MS (p<0.05). The AI results were higher in the ES period than the others (p<0.05). While the Ci (p<0.01) and LP (p<0.001) parameters had the highest values in the BS period, a gradual decrease has been observed until the ES period. In conclusion, it has been observed that the best semen quality can be obtained in the period of MS (15 April – 15 May) for young (5-10) aged Arabian stallions breeding in Türkiye.

Keywords

Arabian stallion, breeding season, computer assisted sperm analysis, flowcytometry, semen quality

Ethical Statement

Ankara University Animal Experiments Local Ethics Committee (No:2022-20-178)

Supporting Institution

The Scientific and Technological Research Council of Turkiye (TUBITAK)

Project Number

122O897

Arap aygırlarında spermatolojik parametrelerin sezonun farklı dönemlerine göre değişimlerinin değerlendirilmesi

Abstract

Bu çalışmada, Arap aygırlarında aşım sezonu içerisinde farklı dönemlerde spermatolojik parametrelerde gözlemlenen farklılıkların belirlenmesi amaçlanmıştır. Aşım sezonu içerisindeki farklı dönemler sezon başı (SB: 15 Şubat – 15 Mart), sezon ortası (SO: 15 Nisan – 15 Mayıs) ve sezon sonu (SS: 30 Mayıs – 30 Haziran) olarak belirlenmiştir. Yaşları 5-10 arasında değişen, vücut kondisyon skorları 3-3,5 arasında olan, genel ve reprodüktif sağlık durumları açısından herhangi bir problem olmayan toplam 7 Arap aygırı çalışmaya dahil edildi. Her bir aygırdan, her bir dönemde bir defa olmak üzere, toplam 3 sperma alma işlemi gerçekleştirildi. Alınan spermalar miktar, yoğunluk, toplam motilite (TMOT), progresif motilite (PMOT), canlılık, akrozom sağlamlığı (AI), yüksek mitokondriyal membran potansiyeli (HMMP), kapasitasyon indeksi (Ci) ve lipid peroksidasyon seviyesi (LPO) parametreleri açısından bilgisayar yardımlı sperma analizi ve akış sitometri yöntemleriyle analiz edildi. Analiz sonuçlarına göre miktar, yoğunluk TMOT ve PMOT parametrelerinde periyotlar arasındaki farklılıklar istatistiksel olarak benzer bulundu (p>0,05). En düşük canlılık değeri SB periyodunda elde edildi (P<0,001). En yüksek HMMP değeri SO periyodunda bulundu (p<0,05). Akrozom sağlamlığı değeri SS periyodunda diğer periyotlara göre daha yüksekti (p<0,05). Kapasitasyon indeksi (p<0,01) ve LPO (p<0,001) değerleri SB periyodunda en yüksek değerlere sahipken, SS periyoduna gidildikçe kademeli olarak düşüş gösterdi. Sonuç olarak, Türkiye’de yetiştiriciliği yapılan genç yaşlı (5-10) Arap aygırlarında en iyi sperma kalitesinin SO periyodunda (15 Nisan – 15 Mayıs) alınabileceği sonucuna ulaşılmıştır.

Keywords

Akış sitometri, Arap aygırı, aşım sezonu, bilgisayar yardımlı sperma analizi, sperma kalitesi

Project Number

122O897

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