Evaluation of bacteria isolated from different animal species and antibiotic resistance in the veterinary diagnostic laboratory

Year 2022, Volume: 1 Issue: 1, 14 - 25, 30.06.2022

Ecehan Aytek Muhammed Furkan Kaplan , Cihan Oz , Anna Levchenko

Abstract

Isolation of bacteria can be performed by taking correct and convenient samples, especially from the infection site. In addition, after isolation of the bacteria, the antimicrobial susceptibility test should be performed routinely to treat animals conveniently. For this purpose, 129 isolates were included in the current study from different animal origins and different examination materials in the culture collection of Atatürk University Faculty of Veterinary Medicine Department of Microbiology between 2020 and 2021. The isolates were identified by bacteriological methods and their antibiotic resistance was evaluated phenotypically. In the study, it was determined that bacteria belonging to the Staphylococcus genus (27.2%) were mostly involved in different infections. Overall results displayed those bacteria tested in this study were resistant to neomycin (100%), penicillin (76.74%), oxytetracycline (73.80%), and sulfamethoxazole-trimethoprim (61.25%) with a different rate, whereas they were susceptible to cephalosporin antibiotics (cefovecin %64.3 ceftiofur %80, and cefoxitin %81.8) used in the current study.

Keywords

Antimicrobial resistance, bacteria, fungi, retrospective study

References

• Adiguzel, M. C., Schaefer, K., Rodriguez, T., Ortiz, J., & Sahin, O. (2022). Prevalence, mechanism, genetic diversity, and cross-resistance patterns of methicillin-resistant Staphylococcus isolated from companion animal clinical samples submitted to a veterinary diagnostic laboratory in the Midwestern United States. Antibiotics, 11(5), 609. https://doi.org/10.3390/antibiotics11050609
• Adiguzel, M. C., Cengiz, S., Cengiz, M., & Hayirli, A. (2021). Pathogenic bacteria present in the lochia first 10− day postpartum prolongs days open in dairy cows. Ataturk University Journal of Veterinary Science, 16(1), 32-40. https://doi.org/10.17094/ataunivbd.845646
• Baran, A., Oz, C., Cengiz, S., & Adiguzel, M. C. (2022). Genomic characterization, antimicrobial resistance profiles, enterotoxin, and biofilm production of methicillin- resistant Staphylococcus aureus isolated from clinical and animal products origins in Eastern Turkey. Pesquisa Veterinária Brasileira, 42, 1-8. https://doi.org/10.1590/1678- 5150-PVB-6991
• Barnes, H. J., Nolan, L. K., Vaillancourt, J. P., & Saif, Y. M. (2008). Colibacillosis In: Saif Y.M. (Ed.), Diseases of Poultry. Blackwell Publishing.
• Campbell, C. K., & Johnson, E. M. (2013). Identification of Pathogenic Fungi. John Wiley & Sons. Clinical and Laboratory Standards Institute (CLSI). (2017). Performance Standards for Antibacterial Susceptibility Testing, (27th ed). CLSI. Wayne, PA, M100-s23.
• Denyer, S. P., Hodges, N. A., & Gorman, S. P. (2008). Hugo and Russell's Pharmaceutical Microbiology. John Wiley & Sons.
• Diren Sigirci, B., Metiner, K., Celik, B., Basaran Kahraman, B., İkiz, S., Bagcigil, A.F., Ozgur, N.Y., & Ak, S. (2019). Dermatophytes isolated from dogs and cats suspected dermatophytoses in Istanbul, Turkey within a 15-year-period: An updated report.
• Kocatepe Veterinary Journal, 12(2), 116-121. https://doi.org/10.30607/kvj.495736 European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2017). Media Preparation for EUCAST Disk Diffusion Testing and for Determination of MIC Values by the Broth Microdilution Method, EUCAST. Basel, Switzerland.
• Goulart, D. B., Beyi, A. F., Wu, Z., Adiguzel, M. C., Schroeder, A., Singh, K., ... & Sahin, O. (2022). Effect of danofloxacin treatment on the development of fluoroquinolone resistance in Campylobacter jejuni in calves. Antibiotics, 11(4), 531. https://doi.org/10.3390/antibiotics11040531
• Hoang, P. H., Awasthi, S. P., DO Nguyen, P., Nguyen, N. L., Nguyen, D. T., Le, N. H., Van Dang, C., Hinenoya, A., & Yamasaki, S. (2017). Antimicrobial resistance profiles and molecular characterization of Escherichia coli strains isolated from healthy adults in Ho Chi Minh City, Vietnam. The Journal of Veterinary Medical Science, 79(3), 479–485. https://doi.org/10.1292/jvms.16-0639
• Jonker, A., & Michel, A. L. (2021). Retrospective study of bacterial and fungal causes of abortion in domestic ruminants in northern regions of South Africa (2006–2016). Australian Veterinary Journal, 99(3), 66-71. https://doi.org/10.1111/avj.13035
• Kakooza, S., Muwonge, A., Nabatta, E., Eneku, W., Ndoboli, D., Wampande, E., Munyiirwa, D., Kayaga, E., Tumwebaze, M. A., Afayoa, M., Ssajjakambwe, P., Tayebwa, D. S., Tsuchida, S., Okubo, T., Ushida, K., Sakurai, K., & Mutebi, F. (2021). A retrospective analysis of antimicrobial resistance in pathogenic Escherichia coli and Salmonella spp. isolates from poultry in Uganda. International Journal of Veterinary Science and Medicine, 9(1), 11–21. https://doi.org/10.1080/23144599.2021.1926056
• Kilic D. (2004) Hayvan beslenmesinde antibiyotik kullanimi ve direnç. Flora, 9(1), 29-36. Larone, D.L. (2002). Medically Important Fungi: A Guide to Identification. ASM Press.
• Ribeiro, M. G., Risseti, R. M., Bolaños, C. A., Caffaro, K. A., de Morais, A. C., Lara, G. H., Zamprogna, T. O., Paes, A. C., Listoni, F. J., & Franco, M. M. (2015). Trueperella pyogenes multispecies infections in domestic animals: a retrospective study of 144 cases (2002 to 2012). Veterinary Quarterly, 35(2), 82–87. https://doi.org/10.1080/01652176.2015.1022667
• Nocera, F.P., Ambrosio, M., Fiorito, F., Cortese, L., De Martino, L. (2021). On gram- positive- and gram-negative-bacteria-associated canine and feline skin infections: a 4-year retrospective study of the university veterinary microbiology diagnostic laboratory of Naples, Italy. Animals. 11(3), 1603. https://doi.org/10.3390/ani11061603
• Quinn PJ, Carter, ME., Markey, BK., Donnelly, WJC., & Leonard, FC. (2004). Clinical Veterinary Microbiology, (5th ed.). Harcourt Publishers Limited.
• Puvarajan, B., Reetha, T. L., Kumar, S. S., & Manickam, R. (2020). A retrospective observational study of the prevalence of clinical conditions with special reference to antimicrobial resistance pattern of coliform mastitis in cows and otitis in canines. Journal of Entomology and Zoology Studies, 8(2), 287-289.
• Razali, K., Kaidi, R., Abdelli, A., Menoueri, M. N., & Ait-Oudhia, K. (2020). Oral flora of stray dogs and cats in Algeria: Pasteurella and other zoonotic bacteria. Veterinary World, 13(12), 2806–2814. https://doi.org/10.14202/vetworld.2020.2806-2814
• Srivastava, S. (2013). Genetics of Bacteria. Springer India. https://doi.org/10.1007/978-81- 322-1090-0
• Terziev, G., & Urumova V. (2018). Retrospective study on the etiology and clinical signs of canine otitis. Comparative Clinical Pathology, 27, 7–12. https://doi.org/10.1007/s00580-017-2528-x