Hipokampus ve Serumda NO, H2S ve CO'nun Tiyol/Disülfit Dengesi ve İleri Oksidasyon Protein Ürünleri Üzerindeki Etkileri

Year 2023, Volume: 4 Issue: 1, 7 - 12, 31.05.2023

Çiğdem Çiçek , Veysel Baskın , Kevser Erol

Abstract

Giriş: Nitrik oksit (NO), hidrojen sülfür (H2S) ve karbon monoksit (CO) gaz otokoidler olarak bilinir. Eksojen NO, H2S ve CO uygulamasının hipokampus ve serumda tiyol/disülfit dengesini ve ileri oksidasyon protein ürünlerini (AOPP'ler) nasıl değiştirdiği açık değildir.
Gereç ve Yöntem: Çalışmada, sıçanlara NO donörü olarak L-arginin (100 mg/kg), H2S donörü olarak NaHS (10 mg/kg) ve CO donörü olarak CORM-2 (10 mg/kg) ( trikarbonildiklororutenyum dimer) enjekte edildi. Hipokampus ve serum örneklerinde tiyol/disülfit dengesi ve ileri protein oksidasyon ürünleri (AOPPs) analiz edildi.
Bulgular: L-arginin grubunun hipokampüsündeki serbest tiyol seviyesi, kontrol grubunun serbest tiyol seviyesine göre istatistiksel olarak azaldı (****p≤0.0001). L-arginin grubunun hipokampüsündeki disülfid düzeyi kontrol grubuna göre istatistiksel olarak yüksek bulundu (***p=0,009). NaHS grubu ve CORM-2 grubunun hipokampal total tiyol düzeyi istatistiksel olarak artarken (sırasıyla ***p=0,008,*p=0,0157), CORM-2 grubunun serum disülfit düzeyi azaldı (***p=0,0005). NaHS grubunun serum ve hipokampus AOPP'leri kontrol grubuna göre istatistiksel olarak yüksekti (sırasıyla ***p=0,0006, **p=0,0047). Benzer şekilde CORM-2 grubunda hipokampal AOPPs düzeyi kontrol grubundaki AOPPs düzeyine göre istatistiksel olarak yüksek bulundu (*p=0,0437).
Sonuç: NaHS, tiyol/disülfit dengesini iyileştirebilirken, CORM-2 ve L-Arginin için yeni çalışmalara ihtiyaç vardır. Bu çalışma, NO, H2S ve CO'nun hipokampus ve serumdaki tiyol/disülfit dengesi ve AOPPs'ler üzerindeki etkilerini bildiren ilk çalışmadır.

Keywords

NO, H2S, CO, AOPPs, Tiyol/Disülfit

Supporting Institution

Eskişehir Osmangazi Üniversitesi ve Yüksek İhtisas Üniversitesi

Project Number

2018-2415 ve 2021/01.004

Effects of NO, H2S and CO on Thiol/Disulfide Balance and Advanced Oxidation Protein Products in Hippocampus and Serum

Abstract

Introduction: Nitric oxide (NO), hydrogen sulfide (H2S), and carbon monoxide (CO) are known as gaseous autocoids. It is not clear how the application of exogenous NO, H2S and CO alters the thiol/disulfide balance and advanced oxidation protein products (AOPPs) in the hippocampus and serum.
Materials and Methods: In the study, rats were exogenously injected with L-arginine (100 mg/kg) as a NO donor, NaHS (10 mg/kg) as a H2S donor, and CORM-2 (10 mg/kg) as a CO donor (II) (a tricarbonyldichlororuthenium dimer). Thiol/disulfide balance and advanced protein oxidation products were analyzed in hippocampus and serum samples.
Results: The native thiol level in the hippocampus of the L-arginine group was statistically decreased compared to the native thiol level of the control group (****p≤0.0001). The disulfide level in the hippocampus of the L-arginine group was statistically increased compared to the control group (***p=0.009). Hippocampal total thiol level of NaHS group and CORM-2 group increased statistically (***p=0.008,*p=0.0157, respectively), while serum disulfide level of CORM-2 group decreased (***p=0.0005). Serum and hippocampus AOPPs levels of the NaHS group were statistically increased compared to the control group (***p=0.0006, **p=0.0047, respectively). Similarly, the hippocampal AOPP level in the CORM-2 group was found to be statistically increased compared to the AOPP level in the control group (*p=0.0437).
Conclusion: As NaHS can improve thiol/disulfide balance, new studies are needed for CORM-2 and L-Arginine. This study is the first to report the effects of NO, H2S and CO on thiol/disulfide balance and AOPPs in the hippocampus and serum.

Keywords

NO, H2S, CO, AOPPs, Thiol/disulfide

Project Number

2018-2415 ve 2021/01.004

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