DENEYSEL KOLİT MODELİNDE KARACİĞER VE PANKREASTA GÖRÜLEN MORFOMETRİK DEĞİŞİKLİKLER VE VAGAL STİMULASYONUN KRONİK DÖNEMDE BU DEĞİŞİKLİKLER ÜZERİNE OLAN ETKİLERİNİN İNCELENMESİ

Year 2024, Volume: 5 Issue: 1, 16 - 23, 31.05.2024

Özgenur Koçak , Rabet Gozil , Kerem Atalar , Saadet Özen Akarca Dizakar , Ece Alım , Ayşe Soylu , Meltem Bahcelıoglu

Abstract

Giriş: İnflamatuvar bağırsak hastalığı, sindirim sisteminin kronik ve idiyopatik bir hastalığıdır. Hastalık ayrıca karaciğeri ve pankreası da etkilemektedir. Çalışmadaki amacımız transkutanoz aurikular vagal sinir stimulasyonunun (TAVNS) karaciğer ve pankreas hasarının iyileşmesi üzerindeki etkisini incelemektir.

Materyal ve Metot: Çalışmamız 4 grup halinde 36 sıçanla yapılmıştır. Sham grubuna intrakolonik salin enjeksiyonu yapılmış ve TAVNS uygulanmamıştır. Sham+ TAVNS grubuna intrakolonik olarak salin enjekte edilmiş ve TAVNS uygulanmıştır. TNBS+Sham grubuna intrakolonik olarak TNBS (trinitrobenzen sülfonik asit) enjekte edilmiş ve TAVNS uygulanmamıştır. TNBS+TAVNS grubuna hem TNBS enjekte edilmiş hem de TAVNS uygulanmıştır. Karaciğer dokusu ve pankreas dokusu histolojik ve histomorfometrik olarak incelenmiştir.

Bulgular: Çalışmamızda TNBS+Sham ve TNBS+TAVNS gruplarının son vücut ağırlıkları Sham ve Sham+TAVNS gruplarına göre anlamlı olarak düşük bulundu. TNBS uygulanan grupların karaciğer ve pankreas histopatolojik skorları anlamlı olarak yüksekti. TNBS+Sham grubundaki sıçanların karaciğer hepatositlerinde bazı bölgelerde nekrotik alanlar, vakuolar dejenerasyon ve sinüzoidal konjesyon gözlendi. Grup TNBS+TAVNS grubunun karaciğer kesitlerindeki dejeneratif bulgular kısmen azaldı. TNBS+Sham ve TNBS+TAVNS gruplarındaki hayvanların pankreasındaki Langerhans adacıklarının sayısı ve alanı, Sham ve Sham+ TAVNS gruplarına göre daha düşük bulundu.

Sonuç: Bu çalışmada sıçanlarda TNBS kaynaklı kolitin karaciğer ve pankreasta histopatolojik ve histomorfometrik değişikliklere neden olarak kilo kaybına yol açtığı ve TAVNS'nun bu değişiklikler üzerinde terapötik etkilerinin olduğu tespit edilmiştir.

Keywords

kolit, vagal sinir stimulasyonu, karaciğer, pankreas

Project Number

TTU-2021-7301

Morphometric Changes in Liver and Pancreas in Experimental Colitis Model and Examination of the Effects of Vagal Stimulation on These Changes in Chronic Period

Abstract

Introduction: Inflammatory bowel disease is a chronic and idiopathic disease of the digestive tract. The disease also affects the liver and pancreas. Our aim in the study was to examine the effect of transcutaneous auricular vagal nerve stimulation (TAVNS) on the healing of liver and pancreas damage.

Material and Method: 36 rats in 4 groups were included in this study. The Sham group was intracolonic injected with saline and TAVNS was not applied. The Sham+ TAVNS group was injected intracolonically with saline and TAVNS was applied. The TNBS+Sham group was injected with TNBS (trinitrobenzene sulfonic acid) intracolonically and TAVNS was not applied. In TNBS+ TAVNS group both TNBS was injected and TAVNS was applied. Liver tissue and pancreas tissue were examined histologically and histomorphometrically.

Results: In our study, the final body weights of TNBS+Sham and TNBS+ TAVNS groups were found to be significantly lower than sham and Sham+TAVNS groups. The liver and pancreas histopathological scores of the TNBS injected groups were significantly higher. In the liver hepatocytes of rats in TNBS+Sham group, necrotic areas, vacuolar degeneration, and sinusoidal congestion were observed in some regions. Degenerative findings in liver sections of group TNBS+ TAVNS group were also partially reduced. The number and area of Langerhans islets in the pancreas of the animals in TNBS+Sham and TNBS+ TAVNS groups were found to be lower than in Sham and Sham+ TAVNS groups.

Conclusions: In this study, we found that TNBS-induced colitis in rats caused histopathological and histomorphometric changes in the liver and pancreas, causing weight loss, and that TAVNS had therapeutic effects on these changes.

Keywords

colitis, vagal nerve stimulation, liver, pancreas

Ethical Statement

Ethical approval of the study was obtained from the Animal Research Committee at Ankara Gazi University (G.Ü.ET 21.041).

Supporting Institution

The study was supported by Gazi University Scientific Research Projects Unit with project number TTU-2021-7301.

Project Number

TTU-2021-7301

Thanks

We extend our thanks to Gazi University and Yüksek İhtisas University.

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