Fitoplankton Sınıflarının Belirlenmesinde HPLC ve Spektroflorometrik Yöntemlerin Karşılaştırılması

Year 2017, Volume: 17 Issue: 2, 163 - 170, 01.06.2017

Nebil Yücel

https://doi.org/10.17693/yunusae.vi.286302

Abstract

Bu çalışmada fitoplankton
gruplarının tespit edilmesinde yaygın olarak kullanılan Yüksek Performanslı
Sıvı Kromatografik (HPLC) ve spektroflorometrik metotlar kıyaslanmıştır. Bu
amaçla Mersin Körfezi – Rodos Adası arasında farklı ekosistemleri temsilen 5
farklı istasyondan 4 farklı dönemde (Temmuz-Eylül 2012 ve Mart-Mayıs 2013) 152
adet su örneği alınarak farklı tip filtrelere süzülmüştür. Klorofil-a, pikoplankton, nanoplankton ve
mikroplankton parametreleri her iki metotla ölçülerek kıyaslanmıştır. HPLC
cihazının spektroflorometreye göre daha hassas olduğu fakat yöntemde kullanılan
GF/F filtrelerin pikoplanktonik hücreleri tutmada başarısız olduğu
belirlenmiştir. Akdeniz gibi pikoplankton yoğunluğunun fazla olduğu denizlerde
kullanılan GF/F filtreler, nükleopor polikarbonat filtrelere göre biyokütle
kaybına neden olarak yanlış okumalara neden olabilmektedir. Toplam klorofil-a ve mikroplankton ölçümlerinde her iki
yöntem birbirleriyle uyumlu bulunmuştur. 

Keywords

Klorofil-a, Pigment, Pikoplankton, Nanoplankton, Mikoplankton

Comparison of HPLC and Spectrofluorometric Method for Determination of Phytoplankton Classess

Abstract

In this study, High Performance Liquid
Chromatographic (HPLC) and Spectrofluorometric methods commonly used in
determining of phytoplankton groups were compared. For this purpose, totally
152 water samples were collected in four different periods (July-September 2012
and March-May 2013) from five stations representing different ecosystems between
Mersin Bay and Rhodes Island filtered by using different types of filters.
Chlorophyll a, picoplankton,
nanoplankton and microplankton parameters were compared by using HPLC and
spectrofluorometric methods. The HPLC was found to be more sensitive than the spectrofluorometer,
but it was determined that the GF/F filters used in the method failed to retain
the picoplanktonic cells. GF/F filters used in seas where the concentration of
picoplankton is high, such as the Mediterranean, may cause biomass loss as compared
to nucleopore polycarbonate filters, causing false readings. Chlorophyll-a and microplankton measurements were
found to be compatible with each other when using both methods.

Keywords

Cholorophyll-a, Pigments, Picoplankton, Nanoplankton, Microplankton

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