Automatic Cells Counting in Natt-Herrick Stained Fish Blood

Year 2017, Volume: 17 Issue: 3, 283 - 294, 01.09.2017

M. Ozan İncetaş , Erdinç Veske Nesrin Emre , Recep Demirci

https://doi.org/10.17693/yunusae.v17i30729.306490

Abstract

Monitoring of hematological values which provide important information about
the health status of fish is considerably important in aquaculture. One of the
most commonly used methods for detecting the hematological values in fish blood
is the usage of Natt-Herrick solution.   Basically, in this approach, Natt-Herrick
stained blood samples are examined with a microscope and the cells are counted.
Nevertheless, the counting process is both tough and time-consuming. In this
study, a technique in which cell counting in blood samples images is
automatically performed has been presented. Natt-Herrick stained blood samples of
Oncorhynchus mykiss and Sparus aurata were used for evaluation
of the developed scheme. The outputs generated by automatic blood cells detection
algorithm in 90 images were compared with results which were obtained by means
of user’s intervention.  Consequently, an
average f-score over 0.96 was achieved.

Keywords

Fish blood, Natt-Herrick solution, Automatic cell counting

Automatic Cells Counting in Natt-Herrick Stained Fish Blood

Abstract

Hematolojik değerlerin kontrolü balıkların
sağlık durumları hakkında önemli bilgiler verdiğinden, bu kontrol balık
yetiştiriciliğinde oldukça önemlidir. Hematolojik değerlerin kontrolünde en sık
kullanılan yöntemlerden biri de Natt-Herrick solüsyonudur. Natt-Herrick
solüsyonu yardımıyla boyanan kan örnekleri mikroskop ile incelenmekte ve hücre
sayımı yapılmaktadır. Ancak bu sayım hem yorucu hem de zaman alıcıdır. Bu
çalışmada kan örneklerine ait görüntüler üzerinden otomatik olarak hücre sayımı
yapabilecek bir teknik sunulmuştur. Geliştirilen yöntemin değerlendirilmesinde
Natt-Herrick solüsyonu ile boyanmış Gökkuşağı Alabalığı ve Çipura balıklarına
ait örnekler kullanılmıştır. Bu örneklere ait 90 adet görüntüdeki kan
hücrelerinin otomatik belirlenmesi sonuçları ile kullanıcılar tarafından
belirlenen sonuçlarla karşılaştırılmıştır. Karşılaştırma sonucunda ortalama
olarak 0,96’nın üzerinde f-skor değeri elde edilmiştir.

Keywords

Balık kanı, Natt-Herrick solüsyonu, Otomatik hücre sayımı

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