Gökkuşağı Alabalıklarından (Oncorhynchus mykiss) İzole Edilen Patojen Bakteri İzolatlarının Hidrolitik Enzim Aktiviteleri ve Siderofor Üretim Yetenekleri

Year 2022, Volume: 27 Issue: 3, 512 - 520, 25.12.2022

Bilgin Taşkın , Şükrü Önalan

https://doi.org/10.53433/yyufbed.1082784

Abstract

Kısa sürede büyük miktarlarda kültürlenebilmeleri, genetik manipülasyonlara açık olmaları ve ürettikleri enzimlerin hayvansal ve bitkisel enzimlerden daha aktif ve istikrarlı oluşu bakterilerin çok önemli enzim ve ikincil metabolit kaynakları olarak değerlendirilmelerine sebep olmaktadır. Ayrıca ekstraselüler hidrolitik enzimler ve sideroforlar patojen bakteriler için çok önemli virülans faktörleri olarak bilinmektedir. Bu çalışmada, Van ilinde faaliyet gösteren çiftliklerde, gökkuşağı alabalıklarından (Oncorhynchus mykiss) izole edilerek kültüre alınmış dokuz adet bakteri izolatının çeşitli ekstraselüler hidrolitik enzimleri ve siderofor üretme yetenekleri ilk defa araştırılmıştır. Sonuçlar, test edilen izolatlardan dokuzunun proteaz, sekizinin lipaz, beşinin selülaz, ikisinin pektinaz aktivitesine sahip olduğu, ayrıca yedisinin siderofor üretme yeteneğine sahip olduğunu göstermiştir. Amilaz ve ksilinaz aktiviteleri hiçbir izolatta gözlemlenmemiştir. Ayrıca bu izolatlar morfolojik olarak olası üç ana gruba ayrılmış ve her bir izolat, türe özel geliştirilmiş primerler ile moleküler tanımlamaya tabi tutulmuştur. Türe özgü primerler kullanılarak yapılan moleküler tanımlama, bu izolatların balık patojeni Listonella anguillarum, Yersinia ruckeri ve Lactococcus garvieae olduklarını ortaya koymuştur.

Keywords

Ekstraselüler enzim, Patojen bakteri, Siderofor

Thanks

Bu çalışmada pozitif kontrol olarak kullanılan Y. ruckeri kültür izolatları Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenen FBA-2018-6895 numaralı projeden, L. garvieae ve L. anguillarum kültür izolatları ise Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenen FYL-2019-7463 numaralı projeden kullanılarak gerçekleştirilmiştir.

Hydrolytic Enzyme Activities and Siderophore Production Capabilities of Pathogenic Bacterial Isolates from Rainbow Trout (Oncorhynchus mykiss)

Abstract

The fact that they can be cultured in large quantities in a short time, that they are open to genetic manipulations, and that the enzymes they produce are more active and stable than animal and plant enzymes, cause bacteria to be considered as very important enzyme and secondary metabolite sources. Also, extracellular hydrolytic enzymes and siderophores are known as very important virulence factors for pathogenic bacteria. In this study, the ability to produce various extracellular hydrolytic enzymes and siderophores of nine bacterial isolates isolated from rainbow trout (Oncorhynchus mykiss) from farms operating in Van province was investigated for the first time. The results showed that of the tested bacteria, nine had protease, eight had lipase, five had cellulase, two had pectinase activity, and seven had the ability to produce siderophores. Amylase and xylanase activities were not observed in any isolate. In addition, these isolates were morphologically divided into three possible main groups and three isolates from each group were molecularly identified with primers developed specifically for the species. Molecular identification using species-specific primers revealed that these isolates could be the strains of fish pathogens Listonella anguillarum, Yersinia ruckeri and Lactococcus garvieae.

Keywords

Extracellular enzymes, Pathogenic bacteria, Siderophore

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