Çilekte Botrytis cinerea’ya Karşı Bakterilerin Antagonist Etkilerinin In Vitro Koşullarda Belirlenmesi

Year 2022, Volume: 27 Issue: 3, 535 - 547, 25.12.2022

Tuba Genç Kesimci , Mesude Figen Dönmez

https://doi.org/10.53433/yyufbed.1089390

Cited By: 1

Abstract

Bu çalışmada, çilekte hasat öncesi ve hasat sonrası ürün kayıplarına neden olan Botrytis cinerea’ya karşı Iğdır ilinde tuzlu topraklardan ve Phragmites australis bitkisinden izole edilen bakteri strainlerinin antagonistik özellikleri araştırılmıştır. Yapılan izolasyonlar neticesinde 89 bakteri straini elde edilmiş ve bu bakterilerin tanıları yağ asit metil ester analizi ile yapılmıştır. Elde edilen bakteriler arasında 38 strain in vitro testlerde etkili bulunmuş ve bu strainlerin B. cinerea’nın misel gelişimini farklı oranlarda engelledikleri tespit edilmiştir. Başarılı olan strainler cins düzeyinde değerlendirildiğinde Bacillus ve Paenibacillus cinslerinin daha etkili oldukları belirlenmiştir. Çalışmada kullanılan aday antagonistlerin inhibisyon oranlarına bakıldığında en etkili strainin %71.68 oranı ile Bacillus subtilis MFD2-21 olduğu, bu straini %68.89 oranı ile Paenibacillus polymyxa MFD-15 ve %61.93 oranı ile Paenibacillus apiarus MFD20 straininin takip ettiği belirlenmiştir. B. cinerea’nın misel gelişimini en düşük oranda engelleyen aday antagonist strain ise %17.36 oranı ile Bacillus sphaericus GC subgroup E MFD3-15 olarak saptanmıştır.

Keywords

Bacillus subtilis, Biyolojik mücadele, Botrytis cinerea, Çilek, In vitro

Determining of the Antagonist Effects of Bacteria Againts Botrytis cinerea in Strawberry under In Vitro Conditions

Abstract

In this study were investigated the antagonistic activities of bacterial strains isolated from salty soils and Phragmites australis plant in Iğdır province against Botrytis cinerea in strawberry. As a result of the isolations, 89 bacterial strains were obtained and the determination of the bacteria was made by fatty acid methyl ester analysis. Among them, 38 bacteria strains were found to be effective in in vitro tests and it was determined that the strains were inhibited mycelial growth of B. cinerea at different rates when successful strains were evaluated at the genus level, it was determined that Bacillus and Pseudomonas were two of the most important genus. Considering the inhibition rates of the putative antagonists were determined that the most effective strains were Bacillus subtilis MFD2-21 (71.68%) Paenibacillus polymyxa MFD-15 (68.89%) and Paenibacillus apiarus MFD20 (61.93%), respectively. The putative antagonist strain that inhibits mycelial growth of B. cinerea at the lowest rate was determined as Bacillus sphaericus GC subgroup E MFD3-15 (17.36%).

Keywords

Bacillus subtilis, Biological control, Botrytis cinerea, In vitro, Strawberry

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