The Applicability of Wheat SSR Markers to Analyzing the Molecular Diversity and Distribution of Orchardgrass (Dactylis glomerata L.) Genotypes from Eastern Anatolian Habitats

Year 2023, Volume: 28 Issue: 2, 732 - 744, 31.08.2023

Ahmad Nabhan , Ösmetullah Arvas , M. Alp Furan

https://doi.org/10.53433/yyufbed.1260839

Abstract

Türkiye is one of the world's nations with the greatest flora diversity in different plants. Moreover, it possesses a high level of plant genetic diversity. Orchardgrass (Dactylis glomerata L.) is one of the most important fodder species used during cool seasons since it is a hardy and perennial plant. The identification of Orchardgrass cultivars is essential for maximizing cultivar utilization, and safeguarding breeders' intellectual property. Dactylis glomerata L. is an allogamous, variable, monospecific genus with multiple subspecies distinguished by morphology, chromosomal count, and distribution. This genus has a single species, Dactylis glomerata L, which is comprised of multiple subspecies whose traits have not been exhaustively characterized. Using DNA assays that evaluated the transferability of nine SSR primers designed for wheat loci, the genetic diversity of 44 orchardgrass genotypes from eight naturally distributed locales in the Eastern Anatolia Area of Türkiye was calculated. On average, 6.78 alleles were discovered for each of the nine SSR primers, for a total of 61 alleles. A total of 54 polymorphic alleles were identified, with an average of 6.78 per primer. Polymorphism information content (PIC) values ranged from 0.320 (WMC96) to 0.626% (XBARC187). The average polymorphism rate of 88.89% suggests a high amount of genetic diversity among all studied genotypes. The average expected heterozygosity (He) ranged between 0.178 (Ağrı) to 0.882 (Erzurum). The genetic separation ranged from 0.01 to 0.66. In conclusion, our findings indicate that the Dactylis glomerata L genotypes gathered in Eastern Anatolia are a rich source of genetic variability, supplying a vast array of genetic material for orchardgrass breeding efforts.

Keywords

Dactylis glomerata, Genetic diversity, Orchardgrass, SSR

Supporting Institution

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri koordinasyon Birimi

Project Number

FDK-2019_7650

Thanks

The authors extend their appreciation for providing necessary research facilities and support for the completion of this work to Van Yuzuncu Yil University.

Doğu Anadolu Habitatlarından Domuz ayrığı (Dactylis glomerata L.) Türlerinin Moleküler Çeşitliliğinin ve Dağılımının Analizinde Buğday SSR Markerlerinin Uygulanabilirliği

Abstract

Türkiye, dünyanın flora çeşitliliği en fazla olan ülkelerinden biridir. Ayrıca, yüksek düzeyde bitki genetik çeşitliliğine sahiptir. Domuz ayrığı (Dactylis glomerata L.), dayanıklı ve çok yıllık bir bitki olması nedeniyle serin mevsimlerde kullanılan en önemli yem türlerinden biridir. Orchardgrass çeşitlerinin tanımlanması, çeşit kullanımını en üst düzeye çıkarmak ve yetiştiricilerin fikri mülkiyetini korumak için gereklidir. Dactylis glomerata L., morfoloji, kromozom sayısı ve dağılımı ile ayırt edilen çok sayıda alt türü olan allogam, değişken, monospesifik bir cinstir. Bu cinsin tek bir türü vardır, Dactylis glomerata L, özellikleri kapsamlı bir şekilde karakterize edilmemiş birden fazla alt türden oluşur. Buğday lokusları için tasarlanan dokuz SSR primerinin aktarılabilirliğini değerlendiren DNA tahlilleri kullanılarak, Türkiye'nin Doğu Anadolu Bölgesi'nde doğal olarak dağılmış sekiz yerel bölgeden 44 Domuz ayrığı genotipinin genetik çeşitliliği hesaplandı. Ortalama olarak, dokuz SSR primerinin her biri için toplam 61 alel için 6.78 alel keşfedildi. Primer başına ortalama altı olmak üzere toplam 54 polimorfik alel tanımlandı. Polimorfizm bilgi içeriği (PIC) değerleri %0,320 (WMC96) ile %0,626 (XBARC187) arasında değişmektedir. %88.89'luk ortalama polimorfizm oranı, çalışılan tüm genotipler arasında yüksek miktarda genetik çeşitlilik olduğunu göstermektedir. Beklenen ortalama heterozigotluk (He) 0,178 (Ağrı) ile 0,882 (Erzurum) arasında değişmekteydi. Genetik ayırma 0.01 ile 0.66 arasında değişmektedir. Sonuç olarak, bulgularımız, Doğu Anadolu'da toplanan Dactylis glomerata L. genotiplerinin, Domuz ayrığı bitkisi ıslah çabaları için çok çeşitli genetik materyal sağlayan zengin bir genetik çeşitlilik kaynağı olduğunu göstermektedir.

Keywords

Dactylis glomerata, Genetik çeşitlilik, Meyve bahçesi çimi, SSR

Project Number

FDK-2019_7650

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