Molecular based identification and association of Mycobacterium tuberculosis and hepatitis C virus in patient samples of Muzaffargarh, Pakistan

Year 2020, Volume: 1 Issue: 3, 1 - 6, 30.09.2020

Naveed Bashır Muhammad Safdar , Mehmet Özaslan

Abstract

Mycobacterium tuberculosis and Hepatitis C virus are the major diseases spreading more rapidly all over the Pakistan and its load increasing day by day. The main aim of the topic is to determine the correlation of Hepatitis C virus infection among the patient of M. tuberculosis and also to increase the awareness about co infection of HCV in M. tuberculosis patients. The study was cross-sectional and convenient sampling technique was used to approach the patients. In the study the basically patients were separated into different groups of male, female, age group (of <20 years, 20 to 40 years and > 40 years), married, unmarried, financially good, poor, literate and illiterate. The fresh EDTA samples of M. tuberculosis positive patients were taken to screen HCV infection. Further the HCV positive plasma was stored at -210C for later confirmation by PCR. The RNA was extracted first and converted into cDNA for amplification and detection. The standard curve was obtained by HCV reagent RT-PCR Kit for confirmation of qPCR assay. Finally CT value was used to get patients results. Among the 534 M. tuberculosis patients confirmed by genexpert in sputum, there were 94 patients of HCV infection. Overall the burden of HCV infection was 17.60%. The correlation of HCV co infection in male group was 49.46%, in female 50.54%, in literate was 31%, in illiterate 69%, age group < 20 years had 9.57% co-infection, age group 20-40 years had 39.36%, >40 years 51.01%, among married and unmarried the HCV co-infection was 17.03% and 82.97% respectively. There was very high correlation of HCV and M. tuberculosis infection in Muzaffargarh district. The M. tuberculosis patients were less immune to HCV infection as compared to non M. tuberculosis patients. The findings helps to give more awareness to physician to put more efforts towards M. tuberculosis patients who more prone to HCV infection and more work in future are needed to save the M. tuberculosis patients from getting HCV infection.

Keywords

M. tuberculosis, RNA extraction, qPCR, Identification

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