Methylene-Tetrahydrofolate Reductase 677 and 1298 Variations in Relation to Recurrent Abortion

Year 2022, Volume: 3 Issue: 3, 20 - 26, 24.08.2022

Belan Kanabe , Mehmet Özaslan

https://doi.org/10.55549/zbs.1167427

Abstract

Recurrent abortion is multifactorial involving clinical and biological risk factors. Evidence addressed the relationship of inherited thrombophilia with repeated pregnancy abortion and other serious pregnancy complications. However, the relation between thrombophilia associated gene mutations and adverse obstetric outcome is controversial and data in the literature are inconsistent. Main Purpose of this research was to exanimate the prevalence of Methylene-Tetrahydrofolate Reductase gene (MTHFR) variations in association with recurrent abortion. A total of 92 samples were screened in the project: 52 women with multiple consecutive abortion and 40 normal controls. DNA of genomic was extracted from whole blood. For evaluate the presence of the both usual A1298C and C677T MTHFR gene variations in the women with recurrent abortion and controls, we employed real time polymerase chain reaction (RT-PCR). There is critical distinction in the pervasiveness of 677T/T genotype among ladies with repetitive premature birth and typical controls (P = 0.001). Consequently, the outcomes show significant difference in MTHFR C677T/A1298C genotype distribution among the healthy and women with recurrent abortion; hence, further examinations on bigger populace and other hereditary changes to more readily comprehend the molecular pathobiology of repeated pregnancy are required.

Keywords

Recurrent abortion, MTHFR 677, MTHFR 1298, RT-PCR

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