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Efficiency of Native Beauveria bassiana and Metarhizium anisopliae Isolates against the Early Instar fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) Larvae

Year 2021, , 4 - 9, 30.11.2021
https://doi.org/10.33724/zm.886120

Abstract

The fall webworm, Hyphantria cunea is a polyphagous species causing significant damage on many plant species. This study was carried out to determine the insecticidal activity of native Beauveria bassiana and Metarhizium anisopliae isolates against early instar larvae of H. cunea in laboratory conditions. B. bassiana and M. anisopliae were sprayed to 1st and 2nd instar larvae at the dose of 1x105 conidia ml-1. Distilled water with 0.03% Tween 80 was applied to the control groups. The number of dead larvae was recorded after 7 days. All trials repeated four times. B. bassiana and M. anisopliae isolates had significant mortalities on 1st and 2nd instar larvae. While B. bassiana isolates YK11 and YK14 killed 75% of the 1st instar larvae, M. anisopliae isolates YK38, YK43 and YK44 killed 70, 77.5 and 80%, respectively. B. bassiana YK11 and YK14 isolates caused 68.61 and 65.83% mortality in the 2nd instar larvae, respectively. M. anisopliae YK38, YK43 and YK44 isolates displayed 55%, 73.89% and 74.17% mortality at the same instar larvae, respectively. It found that B. bassiana and M. anisopliae isolates can be used to control the larvae of H. cunea. However, there are need future studies to increase their efficiency.

References

  • Aker, O., & Tuncer, C. (2016). Efficacy of Metarhizium anisopliae and some entomopathogenic fungi on larvae of fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae). Journal of Entomology and Zoology Studies, 4(5), 171-176.
  • Aker, O., & Kushiyev, R. (2016). Pathogenicity of Metarhizium anisopliae on larvae of fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) at different temperatures. International Journal of Zoology Studies, 1(6): 29-32.
  • Akkuzu, E. & Mol, T. (2006). Amerikan beyaz kelebeği (Hyphantria cunea (Dry.)) üzerine biyolojik ve morfolojik araştırmalar. Süleyman Demirel Üniversitesi Orman Fakültesi Dergisi, 2, 50–57.
  • Azizoglu, U., & Karabörklü, S. (2021). Role of Recombinant DNA Technology to Improve the Efficacy of Microbial Insecticides” In M.A., Khan, & Ahmad, W., (Eds.) Microbes for Sustainable lnsect Pest Management: Hydrolytic Enzyme & Secondary Metabolite (pp. 159– 182). Switzerland: Springer.
  • Batta, Y. A., & Kavallieratos, N. G. (2018). The use of entomopathogenic fungi for the control of stored-grain insects. International Journal of Pest Management, 64(1), 77-87.
  • Bovey, P. (1954). Un nouveau ravageur en Europe: I’Ecaille fileuse (Hyphantria cunea Drury), Journal Forestier Suisse, No. I.
  • de Faria, M. R., & Wraight, S. P. (2007). Mycoinsecticides and Mycoacaricides: a comprehensive list with worldwide coverage and international classification of formulation types. Biological Control, 43, 237-256.
  • Ge, X., He, S., Zhu, C., Wang, T., Xu, Z., & Zong, S. (2019). Projecting the current and future potential global distribution of Hyphantria cunea (Lepidoptera: Arctiidae) using CLIMEX. Pest Management Science, 75(1), 160-169.
  • Gencer, D., Bayramoglu, Z., Nalcacioglu, R., Demirbag, Z., & Demir, I. (2020). Genome sequence analysis and organization of the Hyphantria cunea granulovirus (HycuGV-Hc1) from Turkey. Genomics, 112(1), 459- 466.
  • Goettel, M. S., Eilenberg, J., & Glare, T. (2005). Entomopathogenic fungi and their role in regulation of insect populations. (Ed: L.I. Gilbert, K. Iatrou, S.S. Gill), Comprehensive Molecular Insect Science, Elseiver, Amsterdam.
  • İskender, N. A., Örtücü, S., & Aksu, Y. (2012). Pathogenicity of three isolates of the entomopathogenic fungi Beauveria Bassiana to control Hyphantria cunea Drury Lepidoptera: Arctiidae larvae. Manas Fen Bilimleri Dergisi, 2(13), 15-21.
  • Ito, Y., & Miyashita, K. (1968) Biology of Hyphantria cunea Drury (Lepidoptera: Arctiidae) in Japan. V. Preliminary life tables and mortality data in urban areas. Researches on Population Ecology, 10,177–209.
  • Karabörklü, S., Azizoglu, U., & Azizoglu, Z. B., (2018). Recombinant entomopathogenic agents: a review of biotechnological approaches to pest insect control. World Journal of Microbiology and Biotechnology, 34(1), 14.
  • Karabörklü, S., Altın, N., & Keskin, Y., (2019). Native Entomopathogenic fungi ısolated from Duzce, Turkey and their virulence on the mealworm beetle Tenebrio molitor L. (Coleoptera: Tenebrionidae). Philippines Agricultural Scientist, 102(1), 82-89.
  • Karabörklü, S., Altın, N., Yıldırım, İ., Öztemiz, S., Sadıç, E., & Aydın, Ö. (2020). Bazı yerel entomopatojen fungusların Amerikan beyaz kelebeğine Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) karşı laboratuvar koşullarındaki insektisidal aktivitesi. Türkiye Biyolojik Mücadele Dergisi, 11(1), 119-128.
  • Karabörklü, S., & Altın, N. (2020). Yerel Beauveria bassiana ve Metarhizium anisopliae İzolatlarının Myzocallis coryli ve Corylobium avellanae Üzerindeki Etkinliği. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi, 6(3), 478-485.
  • Keskin, Y., Karabörklü, S., & Altın, N., (2019). Bazı yerel entomopatojen fungusların toprak koşullarındaki etkinliklerinin Tenebrio molitor L. (Col.: Tenebrionidae) larvaları kullanılarak araştırılması. Türkiye Teknoloji ve Uygulamalı Bilimler Dergisi, 2(1), 26-31.
  • Lacey, L. A. & Goettel, M. S. (1995). Current developments in microbial control of ınsect pests and prospects for the early 21st century. Entomophaga, 40(1): 3-27.
  • Lacey, L. A., Grzywacz, D., Shapiro-Ilan, D. I., Frutos, R., Brownbridge, M., & Goettel, M. S. (2015) Insect pathogens as biological control agents: back to the future. Journal of Invertebrate Pathology, 132, 1–41.
  • Lu, H., Song, H., & Zhu, H. (2017). A series of population models for Hyphantria cunea with delay and seasonality. Mathematical Biosciences, 292, 57–66.
  • Meyling, N. V., Arthur, S., Pedersen, K. E., Dhakal, S., Cedergreen, N., & Fredensborg, B. L. (2018). Implications of sequence and timing of exposure for synergy between the pyrethroid insecticide alpha‐cypermethrin and the entomopathogenic fungus Beauveria bassiana. Pest Management Science, 74, 2488– 2495.
  • Rath, A.C. (2000). The use of entomopathogenic fungi for control of termites. Biocontrol Science and Technology, 10, 563-581.
  • Schneider-Orelli (1947). Entomologisches Praktikum: Einführung in die landund forstwirtschaftliche Insektenkunde. HR Sauerlander, Aarau, Switzerland.
  • Schowalter, T. D. & Ring, D. R. 2017. Biology and management of the fall webworm, Hyphantria cunea (Lepidoptera: Erebidae). Journal of Integrated Pest Management, 8(1): 7: 1–6.
  • Sevim, A., Sevim, E., & Demirbağ, Z. (2015). Entomopatojenik fungusların genel biyolojileri ve Türkiye’de zararlı böceklerin mücadelesinde kullanılma potansiyelleri. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8(1), 115-147.
  • Shah, P. A., & Pell, J. K. (2003). Entomopathogenic fungi as biological control agents. Applied Microbiology and Biotechnology, 61, 413–423.
  • Tuncer, C., & Kansu, I. A. 1994. Konukçu Bitkilerin Hyphantria cunea (Drury) (Lepidoptera, Arctiidae)’ya etkileri üzerinde araştırmalar. Türkiye Entomoloji Dergisi, 18(4): 209–222.
  • Tuncer, C., & Mdviani, R. (2014). Hazelnut pests of Silkroad countries, with spesific emphasis on pests of Georgia. Acta Horticulturae, 1032, 175-181.
  • Wakefield, M. E. (2018). Microbial Biopesticides. In C. Athanassiou, & F. Arthur (Eds.), Recent Advances in Stored Product Protection (pp. 143-168). Berlin, Germany: Springer.
  • Wang, W., Zhou, L., Dong, G., & Chen, F. (2020). Isolation and identification of entomopathogenic fungi and an evaluation of their actions against the larvae of the fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae). BioControl, 65(1), 101-111.

Amerikan Beyaz Kelebeği, Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebiidae) Erken Dönem Larvalarına Karşı Yerel Beauveria bassiana ve Metarhizium anisopliae İzolatlarının Etkinliği

Year 2021, , 4 - 9, 30.11.2021
https://doi.org/10.33724/zm.886120

Abstract

Amerikan beyaz kelebeği, Hyphantria cunea polifag bir tür olup meyve üretim sahalarında, park ve bahçeler ile ormanlık alanlarda önemli derecede zarar meydana getirmektedir. Bu çalışma, Amerikan beyaz kelebeği, H. cunea’nın erken dönem larvalarına karşı yerel Beauveria bassiana ve Metarhizium anisopliae izolatlarının öldürücü etkilerinin belirlenmesi amacıyla laboratuvar koşullarında gerçekleştirilmiştir. Beauveria bassiana ve M. anisopliae izolatları 1x105 konsantrasyonda (konidi ml- 1) püskürtme yoluyla 1. ve 2. dönem larvalara uygulanmıştır. Kontrol gruplarına ise içerisinde %0.03’lük Tween 80 bulunan saf su uygulaması yapılmıştır. Uygulamalar 7 gün boyunca takip edilmiş ve ölen larva sayıları kaydedilmiştir. Bütün denemeler dört tekrarlı olarak yürütülmüştür. Beauveria bassiana ve M. anisopliae izolatlarının 1. ve 2. dönem H. cunea larvaları üzerinde önemli oranlarda öldürücü etki gösterdiği belirlenmiştir. Beauveria bassiana YK11 ve YK14 izolatları 1. dönem H. cunea larvalarının %75’ini öldürmüşken, M. anisopliae YK38, YK43 ve YK44 izolatları ise sırasıyla %70, %77.5 ve 80’ini öldürmüştür. H. cunea’nın 2. dönem larvalarında ise B. bassiana YK11 ve YK14 izolatları sırasıyla %68.61 ve %65.83 oranında, M. anisopliae YK38, YK43 ve YK44 izolatları ise sırasıyla %55, %73.89 ve %74.17 oranında öldürücü etki göstermiştir. Hyphantria cunea ile mücadelede B. bassiana ve M. anisopliae izolatlarının kullanılabileceğine yönelik bulgular elde edilmiştir. Ancak izolatların etkinliğini artırmaya yönelik kapsamlı çalışmalara ihtiyaç duyulmaktadır.

References

  • Aker, O., & Tuncer, C. (2016). Efficacy of Metarhizium anisopliae and some entomopathogenic fungi on larvae of fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae). Journal of Entomology and Zoology Studies, 4(5), 171-176.
  • Aker, O., & Kushiyev, R. (2016). Pathogenicity of Metarhizium anisopliae on larvae of fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) at different temperatures. International Journal of Zoology Studies, 1(6): 29-32.
  • Akkuzu, E. & Mol, T. (2006). Amerikan beyaz kelebeği (Hyphantria cunea (Dry.)) üzerine biyolojik ve morfolojik araştırmalar. Süleyman Demirel Üniversitesi Orman Fakültesi Dergisi, 2, 50–57.
  • Azizoglu, U., & Karabörklü, S. (2021). Role of Recombinant DNA Technology to Improve the Efficacy of Microbial Insecticides” In M.A., Khan, & Ahmad, W., (Eds.) Microbes for Sustainable lnsect Pest Management: Hydrolytic Enzyme & Secondary Metabolite (pp. 159– 182). Switzerland: Springer.
  • Batta, Y. A., & Kavallieratos, N. G. (2018). The use of entomopathogenic fungi for the control of stored-grain insects. International Journal of Pest Management, 64(1), 77-87.
  • Bovey, P. (1954). Un nouveau ravageur en Europe: I’Ecaille fileuse (Hyphantria cunea Drury), Journal Forestier Suisse, No. I.
  • de Faria, M. R., & Wraight, S. P. (2007). Mycoinsecticides and Mycoacaricides: a comprehensive list with worldwide coverage and international classification of formulation types. Biological Control, 43, 237-256.
  • Ge, X., He, S., Zhu, C., Wang, T., Xu, Z., & Zong, S. (2019). Projecting the current and future potential global distribution of Hyphantria cunea (Lepidoptera: Arctiidae) using CLIMEX. Pest Management Science, 75(1), 160-169.
  • Gencer, D., Bayramoglu, Z., Nalcacioglu, R., Demirbag, Z., & Demir, I. (2020). Genome sequence analysis and organization of the Hyphantria cunea granulovirus (HycuGV-Hc1) from Turkey. Genomics, 112(1), 459- 466.
  • Goettel, M. S., Eilenberg, J., & Glare, T. (2005). Entomopathogenic fungi and their role in regulation of insect populations. (Ed: L.I. Gilbert, K. Iatrou, S.S. Gill), Comprehensive Molecular Insect Science, Elseiver, Amsterdam.
  • İskender, N. A., Örtücü, S., & Aksu, Y. (2012). Pathogenicity of three isolates of the entomopathogenic fungi Beauveria Bassiana to control Hyphantria cunea Drury Lepidoptera: Arctiidae larvae. Manas Fen Bilimleri Dergisi, 2(13), 15-21.
  • Ito, Y., & Miyashita, K. (1968) Biology of Hyphantria cunea Drury (Lepidoptera: Arctiidae) in Japan. V. Preliminary life tables and mortality data in urban areas. Researches on Population Ecology, 10,177–209.
  • Karabörklü, S., Azizoglu, U., & Azizoglu, Z. B., (2018). Recombinant entomopathogenic agents: a review of biotechnological approaches to pest insect control. World Journal of Microbiology and Biotechnology, 34(1), 14.
  • Karabörklü, S., Altın, N., & Keskin, Y., (2019). Native Entomopathogenic fungi ısolated from Duzce, Turkey and their virulence on the mealworm beetle Tenebrio molitor L. (Coleoptera: Tenebrionidae). Philippines Agricultural Scientist, 102(1), 82-89.
  • Karabörklü, S., Altın, N., Yıldırım, İ., Öztemiz, S., Sadıç, E., & Aydın, Ö. (2020). Bazı yerel entomopatojen fungusların Amerikan beyaz kelebeğine Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) karşı laboratuvar koşullarındaki insektisidal aktivitesi. Türkiye Biyolojik Mücadele Dergisi, 11(1), 119-128.
  • Karabörklü, S., & Altın, N. (2020). Yerel Beauveria bassiana ve Metarhizium anisopliae İzolatlarının Myzocallis coryli ve Corylobium avellanae Üzerindeki Etkinliği. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi, 6(3), 478-485.
  • Keskin, Y., Karabörklü, S., & Altın, N., (2019). Bazı yerel entomopatojen fungusların toprak koşullarındaki etkinliklerinin Tenebrio molitor L. (Col.: Tenebrionidae) larvaları kullanılarak araştırılması. Türkiye Teknoloji ve Uygulamalı Bilimler Dergisi, 2(1), 26-31.
  • Lacey, L. A. & Goettel, M. S. (1995). Current developments in microbial control of ınsect pests and prospects for the early 21st century. Entomophaga, 40(1): 3-27.
  • Lacey, L. A., Grzywacz, D., Shapiro-Ilan, D. I., Frutos, R., Brownbridge, M., & Goettel, M. S. (2015) Insect pathogens as biological control agents: back to the future. Journal of Invertebrate Pathology, 132, 1–41.
  • Lu, H., Song, H., & Zhu, H. (2017). A series of population models for Hyphantria cunea with delay and seasonality. Mathematical Biosciences, 292, 57–66.
  • Meyling, N. V., Arthur, S., Pedersen, K. E., Dhakal, S., Cedergreen, N., & Fredensborg, B. L. (2018). Implications of sequence and timing of exposure for synergy between the pyrethroid insecticide alpha‐cypermethrin and the entomopathogenic fungus Beauveria bassiana. Pest Management Science, 74, 2488– 2495.
  • Rath, A.C. (2000). The use of entomopathogenic fungi for control of termites. Biocontrol Science and Technology, 10, 563-581.
  • Schneider-Orelli (1947). Entomologisches Praktikum: Einführung in die landund forstwirtschaftliche Insektenkunde. HR Sauerlander, Aarau, Switzerland.
  • Schowalter, T. D. & Ring, D. R. 2017. Biology and management of the fall webworm, Hyphantria cunea (Lepidoptera: Erebidae). Journal of Integrated Pest Management, 8(1): 7: 1–6.
  • Sevim, A., Sevim, E., & Demirbağ, Z. (2015). Entomopatojenik fungusların genel biyolojileri ve Türkiye’de zararlı böceklerin mücadelesinde kullanılma potansiyelleri. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8(1), 115-147.
  • Shah, P. A., & Pell, J. K. (2003). Entomopathogenic fungi as biological control agents. Applied Microbiology and Biotechnology, 61, 413–423.
  • Tuncer, C., & Kansu, I. A. 1994. Konukçu Bitkilerin Hyphantria cunea (Drury) (Lepidoptera, Arctiidae)’ya etkileri üzerinde araştırmalar. Türkiye Entomoloji Dergisi, 18(4): 209–222.
  • Tuncer, C., & Mdviani, R. (2014). Hazelnut pests of Silkroad countries, with spesific emphasis on pests of Georgia. Acta Horticulturae, 1032, 175-181.
  • Wakefield, M. E. (2018). Microbial Biopesticides. In C. Athanassiou, & F. Arthur (Eds.), Recent Advances in Stored Product Protection (pp. 143-168). Berlin, Germany: Springer.
  • Wang, W., Zhou, L., Dong, G., & Chen, F. (2020). Isolation and identification of entomopathogenic fungi and an evaluation of their actions against the larvae of the fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae). BioControl, 65(1), 101-111.
There are 30 citations in total.

Details

Primary Language Turkish
Subjects Agricultural, Veterinary and Food Sciences
Journal Section Kabul Edilmiş - Yayına Hazır Olan Makaleler - Erken Görünüm
Authors

Salih Karabörklü 0000-0003-4737-853X

Nedim Altın 0000-0003-1267-7951

Aleyna Bahadır 0000-0002-7104-1817

Publication Date November 30, 2021
Submission Date February 24, 2021
Acceptance Date May 23, 2021
Published in Issue Year 2021

Cite

APA Karabörklü, S., Altın, N., & Bahadır, A. (2021). Amerikan Beyaz Kelebeği, Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebiidae) Erken Dönem Larvalarına Karşı Yerel Beauveria bassiana ve Metarhizium anisopliae İzolatlarının Etkinliği. Ziraat Mühendisliği(373), 4-9. https://doi.org/10.33724/zm.886120