Literature on investment studies show that one of the basic criteria of angel investors regarding a well-founded start-up is the passion of the team they will invest in, alongside many other tangible criteria. This fact raises some questions including how do entrepreneurs transform their enterprise passion into the organizational culture. In this study, passion is defined as a sociological structure which can be examined with dualistic model. In the start-up, construction of this structure and its transforming into the organizational culture are examined. In line with this purpose, enterprises from the earliest Science and Technology Park were selected in Turkey. Current study was carried out in three phases. The data was collected from 9 entrepreneurs and 26 employees by face to face interview and evaluated by critical discourse analysis. According to the findings, the entrepreneurs were classified into four categorizations in terms of their passion. While entrepreneurs who belong to first category could not transform their passion to the organizational culture the most successful entrepreneurs about transformation are in the category four. Successful entrepreneurs can share passion more easily with their employees if they have internalized -not idealized- besides having risk oriented passion.