Mesobuthus gibbosus (Scorpiones: Buthidae) Akrep Ham Zehrinin Kanser Hücre Hatları Üzerine Sitotoksik ve Antiproliferatif Etkilerinin Araştırılması

Yıl 2023, Cilt: 12 Sayı: 1, 19 - 31, 28.04.2023

Nazife Yiğit Kayhan , İlkay Çorak Öcal , Onur Büyükkartal

https://doi.org/10.47493/abantmedj.1094466

Öz

Amaç: Akrep zehirleri, çeşitli kimyasal maddelerin oluşturduğu oldukça kompleks heterojen karışımlardır. Bu çalışmada, Mesobuthus gibbosus (Brullé, 1832) (Scorpiones: Buthidae) akrebinin ham zehrinin kanser hücreleri üzerine sitotoksik ve antiproliferatif etkileri araştırılmıştır.
Gereç ve Yöntemler: M. gibbosus’dan sağılan ham zehrin sitotoksik etkileri MCF-7 meme kanseri ve A549 akciğer karsinomu ve normal fare fibroblast (L929) hücreleri üzerindeki sitotoksik ve antiproliferatif etkileri çalışılmış ve elde edilen veriler değerlendirilmiştir. Sitotoksisiteyi belirlemek için MTT testi, apoptoz ve nekroz indeksini belirlemek için ikili boyama metodu ve xCELLigence gerçek zamanlı analiz sistemi ile hücre proliferasyonunun analizi in vitro koşullarda yapılmıştır.
Bulgular: Çalışmada, akrep ham zehrinin MCF-7 ve A549 kanserli hücrelerin üzerine sitotoksik etki sonuçları ayrı ayrı L929 fibroblast hücre sonuçları ile Mann Whitney U testi kullanılarak istatistiksel analize tabi tutulmuştur. Bunun sonucunda L929 hücrelerinin % canlılık değeri A-549 ‘un değerine çok yakın iken MCF-7 hücrelerinin % canlılık değeri L929’un değerine göre daha yüksek olmasına rağmen araştırmadaki fark, istatistiksel olarak anlamlı bulunmamıştır. Bu hücrelerin proliferasyon grafikleri incelendiğinde ise en yüksek dozdaki zehrin MCF-7 ve L929 hücrelerinin proliferasyonunun bir miktar azalttığı ancak A549 hücresinin proliferasyonunu etkilemediği gözlenmiştir.
Sonuç: M. gibbosus akrep ham zehrinin MCF-7 ve A549 kanserli hücreleri üzerine apoptotik ve antiproliferatif etkileri bir miktar gözlenmiştir. Ancak bu sonuçlar istatiksel olarak anlamlı değildir.

Anahtar Kelimeler

Akrep Zehri, Mesobuthus gibbossus, Kanser, Hücre Proliferasyonu, Sitotoksisite

Investigation of Cytotoxic and Antiproliferative Effects of Mesobuthus gibbosus (Scorpiones: Buthidae) Crude Scorpion Venom on Cancer Cell Lines

Öz

Objective: Scorpion venoms are a highly complex mixture of various chemicals with surprising biological effects. In this study, the cytotoxic and anti-proliferative effects of Mesobuthus gibbosus (Brullé, 1832) (Scorpiones: Buthidae) scorpion crude venom on cancer cell lines were investigated.
Materials and Methods: The cytotoxic and anti-proliferative effects of crude venom milked from M. gibbosus on MCF-7 breast cancer and A549 lung carcinoma, and normal mouse fibroblast (L929) cells were studied and the obtained data were evaluated. The MTT test to determine cytotoxicity, the double staining method to determine the apoptosis and necrosis index, and the analysis of cell proliferation with the xCELLigence real-time analysis system were performed in vitro.
Results: In the study, the results of cytotoxic effects of scorpion crude venom on between MCF-7 and A549 cancer cells and L929 fibroblast cell were analyzed statistically by using Mann Whitely U test separately. As a result, although the % viability value of L929 cells was very close to the value of A-549, the % viability value of MCF-7 cells was higher than the value of L929, but the difference in the study was not statistically significant. When the proliferation graphs of these cells were examined, it was observed that the highest dose of venom slightly reduced the proliferation of MCF-7 and L929 cells, but did not affect the proliferation of A549 cells.
Conclusion: The apoptotic and antiproliferative effects of M. gibbosus scorpion crude venom on MCF-7 and A549 cancer cells were observed to some extent. However, these results are not statistically significant.

Anahtar Kelimeler

Scorpion Venom, Mesobuthus gibbosus, Cancer, Cell Proliferation, Cytotoxic

Kaynakça

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