Inhibitory Effect of Cell Phones Against Human Breast Cancer and Myeloid Leukemia Cells Proliferation in Culture Media

Yıl 2023, Cilt: 6 Sayı: 2, 192 - 197, 30.06.2023

Bircan Boğa , Merve Akbulut , Erkan Maytalman , İlknur Kozanoğlu

https://doi.org/10.53446/actamednicomedia.1177308

Öz

Objective: There is current news that emerges regarding the relationship between the magnetic effects of cell phones and some types of cancer. In spite of the studies carried out, the level of evidence of this news is low, and also the relationship between the magnetic effects of cell phones and other types of cancer is not certain except for brain cancer. In this study, it is aimed at examining the effects of magnetic field of cell phones on the samples of breast cancer human myeloid leukemia cell growth.
Methods: In the study, breast cancer MCF-7 and leukemia K562 cell lines were used as the source of cancer cell. During the six-day cell culture, cancer cells were subjected to the effects of cell phone by using a telephone call program (Automated outbound call software). The system made 6 calls for 1 minute for each call once in 144 minutes from fixed line. The number of cultured cells and proliferation capacities of the two types of tumor cells in the control and experimental groups were assessed.
Results: The number of cancer cells, which were subjected to the effects of cell phone as a result of the culture of tumor cells, was found lower when compared with control group (7500000 ± 100000 vs 6625000 ± 225000 for MCF-7; 15412500 ± 112500 vs 13700000 ± 250000 for K562; P < 0.05 for both). In MTT test, it was found out that two types of cell proliferation was inclined to slow down with the effect of cell phone.
Conclusion: The results can be translated that cell phone may inhibit neoplastic transformation, and this observation may promote to initiate a new clinical studies both for healthy people and for patients with cancer.

Anahtar Kelimeler

Cancer cells, Magnetic fields, Cell phone, Proliferation

Teşekkür

We would like to thank to the managers of Başkent University Adana Teaching and Medical Research Center & Hematology Research Laboratory who supported our study and Telekutu Communication Services Turkey Distributor Salim Özbiçer who provided support in cell phone calls.

Kültür Ortamında İnsan Meme Kanseri ve Miyeloid Lösemi Hücrelerinin Proliferasyonuna Karşı Cep Telefonlarının İnhibitör Etkisi

Öz

Amaç: Cep telefonlarının manyetik etkileri ile bazı kanser türleri arasındaki ilişkiye dair güncel haberler bulunmaktadır. Yapılan araştırmalara rağmen bunların kanıt düzeyi düşüktür. Ayrıca cep telefonlarının manyetik etkilerinin beyin kanseri dışında diğer kanser türleri ile ilişkisi kesin değildir. Bu çalışmada, insan meme kanseri ve miyeloid lösemi hücre örneklerinin proliferasyonu üzerinde cep telefonlarının manyetik alanının etkilerinin incelenmesi amaçlanmıştır.
Yöntemler: Çalışmada kanser hücresi kaynağı olarak meme kanseri MCF-7 ve lösemi K562 hücre dizileri kullanıldı. Altı günlük hücre kültürü sırasında kanser hücreleri, bir telefon arama programı (otomatik giden arama yazılımı) kullanılarak cep telefonunun etkilerine maruz bırakıldı. Sistem sabit hattan 144 dakikada bir her aramada 1’er dakika süreyle 6 arama yaptı. Kontrol ve deney gruplarındaki iki tip tümör hücresinin kültürlenen hücre sayısı ve çoğalma kapasiteleri değerlendirildi.
Bulgular: Tümör hücrelerinin kültürü sonucunda cep telefonu etkisine maruz kalan kanser hücrelerinin sayısı kontrol grubuna göre daha düşük bulundu (7500000 ± 100000 vs 6625000 ± 225000 MCF-7 için; 15412500 ± K562 için 112500 vs 13700000 ± 250000; her ikisi için P < 0.05). MTT testinde iki tip hücre çoğalmasının cep telefonunun etkisiyle yavaşlamaya meyilli olduğu tespit edildi.
Sonuç: Sonuçlar, cep telefonunun neoplastik dönüşümü engelleyebileceği şeklinde tercüme edilebilir ve bu gözlem hem sağlıklı insanlar hem de kanserli hastalar için yeni bir klinik çalışma başlatmayı teşvik edebilir.

Anahtar Kelimeler

Kanser Hücreleri, Manyetik alan, Cep telefonu, Proliferasyon

Kaynakça

• Referans 1. Jiang W, Chen L, Zheng S. Global Reprogramming of Apoptosis-Related Genes during Brain Development. Cells. 2021;10(11):2901. doi: 10.3390/cells10112901.
• Referans 2. Yao C, Zhao L, Peng R. The biological effects of electromagnetic exposure on immune cells and potential mechanisms. Electromagn Biol Med. 2022;41(1):108-117. doi: 10.1080/15368378.2021.2001651.
• Referans 3. Ioniţă E, Marcu A, Temelie M, Savu D, Şerbănescu M, Ciubotaru M. Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination. Sci Rep. 2021;11(1):12651. doi: 10.1038/s41598-021-91790-3.
• Referans 4. Ahearn A. Assessing the science of cell phone safety, with David Savitz. Environ Health Perspect. 2011;119(11):2 p followiing A468. doi: 10.1289/ehp.trp110111.
• Referans 5. Piszczek P, Wójcik-Piotrowicz K, Gil K, Kaszuba-Zwoińska J. Immunity and electromagnetic fields. Environ Res. 2021;200:111505. doi: 10.1016/j.envres.2021.111505.
• Referans 6. Munshi A. Cellular phones: to talk or not to talk. J Cancer Res Ther. 2011;7(4):476-7. doi: 10.4103/0973-1482.92025.
• Referans 7. Phillips JL, Singh NP, Lai H. Electromagnetic fields and DNA damage. Pathophysiology. 2009;16(2-3):79-88. doi: 10.1016/j.pathophys.2008.11.005.
• Referans 8. Hack SJ, Kinsey LJ, Beane WS. An Open Question: Is Non-Ionizing Radiation a Tool for Controlling Apoptosis-Induced Proliferation? Int J Mol Sci. 2021;22(20):11159. doi: 10.3390/ijms222011159.
• Referans 9. Swerdlow AJ, Feychting M, Green AC, Leeka Kheifets LK, Savitz DA; International Commission for Non-Ionizing Radiation Protection Standing Committee on Epidemiology. Mobile phones, brain tumors, and the interphone study: where are we now? Environ Health Perspect. 2011;119(11):1534-8. doi: 10.1289/ehp.1103693.
• Referans 10. Frank JW. Electromagnetic fields, 5G and health: what about the precautionary principle? J Epidemiol Community Health. 2021:jech-2019-213595. doi: 10.1136/jech-2019-213595.
• Referans 11. Mild KH, Wilén J, Mattsson MO, Simko M. Background ELF magnetic fields in incubators: a factor of importance in cell culture work. Cell Biol Int. 2009;33(7):755-7. doi: 10.1016/j.cellbi.2009.04.004.
• Referans Referans 12. Bartosova K, Neruda M, Vojtech L. Methodology of Studying Effects of Mobile Phone Radiation on Organisms: Technical Aspects. Int J Environ Res Public Health. 2021;18(23):12642. doi: 10.3390/ijerph182312642.
• Referans 13. Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura Y, Ueno K. A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. Biol Pharm Bull. 1996;19(11):1518-20. doi: 10.1248/bpb.19.1518.
• Referans 14. Aoyama T, Shibayama Y, Furukawa T, Sugawara M, Takekuma Y. Continuous Cytostatic Effects of BCR-ABL Tyrosine Kinase Inhibitors (TKIs) after Washout in Human Leukemic K562 Cells. Biol Pharm Bull. 2019;42(11):1805-1813. doi: 10.1248/bpb.b19-00185.
• Referans 15. Górski R, Nowak-Terpiłowska A, Śledziński P, Baranowski M, Wosiński S. Morphological and cytophysiological changes in selected lines of normal and cancer human cells under the influence of a radio-frequency electromagnetic field. Ann Agric Environ Med. 2021;28(1):163-171. doi: 10.26444/aaem/118260.
• Referans 16. Cardis E, Richardson L, Deltour I, et all. The INTERPHONE study: design, epidemiological methods, and description of the study population. Eur J Epidemiol. 2007;22(9):647-64. doi: 10.1007/s10654-007-9152-z.
• Referans 17. Castaño-Vinyals G, Sadetzki S, Vermeulen R, et all. Wireless phone use in childhood and adolescence and neuroepithelial brain tumours: Results from the international MOBI-Kids study. Environ Int. 2022;160:107069. doi: 10.1016/j.envint.2021.107069.
• Referans 18. Khan MW, Juutilainen J, Auvinen A, Naarala J, Pukkala E, Roivainen P. A cohort study on adult hematological malignancies and brain tumors in relation to magnetic fields from indoor transformer stations. Int J Hyg Environ Health. 2021;233:113712. doi: 10.1016/j.ijheh.2021.113712.
• Referans 19. Frei P, Mohler E, Neubauer G, et all. Temporal and spatial variability of personal exposure to radio frequency electromagnetic fields. Environ Res. 2009;109(6):779-85. doi: 10.1016/j.envres.2009.04.015.