Different Doses Effects of Trichoderma harzianum and Bacillus subtilis on Young plants 110R/Merlot II. Uprooting Period

Yıl 2018, , 67 - 75, 31.12.2018

İlknur Korkutal , Elman Bahar , Majed Noor Al-deen Mahmood

https://doi.org/10.25308/aduziraat.430557

Cited By: 2

Öz

This study was performed in 2014 at Namik Kemal University Agricultural Faculty Department of Horticulture Practices Area, in order to identify the effects of different doses Bacillus subtilis (0, 2%, 4%, 8%) and Trichoderma harzianum (0, 5g/L, 10g/L, 20g/L) on Merlot/110R two years old young plants. For this purpose, each young plants were dipped into 4 different doses of bio-agents (Sim Bacil and Sim Derma) solution for 5min before they were planted. In this research, diameter characteristics, root and shoot characteristics were evaluated. Bacillus subtilis decreased root fresh and dry weight whereas the other criterias unlike. Trichoderma harzianum decreased main shoot diameter, lateral root fresh weight and total shoot dry weight or positive effected the others. As a result, Bacillus subtilis dose 8% and Trichoderma harzianum dose 5g/L had a positive effect on Merlot/110R graft combination in uprooting period for examined characteristics. Therefore; it is suggested that these doses can be used for further studies.

Anahtar Kelimeler

Merlot, 110R, Bacillus subtilis, Trichoderma harzianum, Vitis vinifera L., uprooting period

110R Anacına Aşılı Merlot Üzüm Çeşidi Genç Omcalarına Farklı Dozlarda Uygulanan Trichoderma harzianum ve Bacillus subtilis’ in II. Söküm Dönemindeki Etkileri

Öz

Bu araştırma 2014 yılında Namık Kemal
Üniversitesi Ziraat Fakültesi Bahçe Bitkileri Bölümü Uygulama arazisinde, 2
yaşındaki Merlot/110R fidanları üzerine farklı dozlarda uygulanan Bacillus subtilis (0, %2, %4, %8) ve Trichoderma harzianum (0, 5g/L, 10g/L,
20g/L)’ un asma fidanlarının çap, kök ve sürgün gelişimi üzerine etkilerinin
belirlenmesi amacıyla yapılmıştır. Her bir fidan 4 farklı dozda biyo-ajan (Sim
Derma ve Sim Bacil) solüsyonuna 5dk süresince batırılıp bekletilmiştir.
Araştırmada çap özellikleri, kök özellikleri ve sürgün özellikleri incelenmiştir.
Bacillus subtilis; dip kök yaş ve
kuru ağırlığı üzerine azaltıcı etkilerde bulunmuş, diğer kriterlerde ise artırıcı
bir etki göstermiştir. Trichoderma
harzianum ise ana sürgün çapı, yan kök yaş ağırlığı ve genel sürgün kuru
ağırlığı üzerine azaltıcı etkiler yapmış; diğer kriterler üzerine ise pozitif
bir etki yapmıştır. Sonuç olarak tüm biyo-ajanlar ve dozları incelendiğinde Bacillus subtilis’ in %8 ve Trichoderma harzianum 5g/L dozunun
Merlot/110R fidanlarında söküm döneminde incelenen kriterler üzerine olumlu
etkiler yaptığı belirlenmiştir. Bu nedenle araştırıcılar tarafından bundan
sonra yapılacak olan çalışmalarda kullanılması önerilebilir.

Anahtar Kelimeler

Merlot, 110R, Bacillus subtilis, Trichoderma harzianum, Vitis vinifera L., söküm dönemi

Kaynakça

• Ağaoğlu YS (1999) Bilimsel ve Uygulamalı Bağcılık Cilt I: Asma Biyolojisi Kavaklıdere Eğitim Yayınları No: 1. Ankara. 205s.
• Al-Zubaidi HK (1992) Almqaoma Alhiowiya llafat. Dar alkutup lltibaa ve alneshir. Jamiat almusul. 440s.
• Arıkan S, Ipek M, Pirlak L (2013) Effects of Plant Growth Promoting Rhizobacteria (PGPR) on Yield and Fruit Quality of Quince. 2013 International Conference on Agriculture and Biotechnology IPCBEE 60. IACSIT Press, Singapore.
• Aslantas R, Cakmakci R, Sahin F (2007) Effect of Plant Growth Promoting Rhizobacteria on Young Apples Trees Growth and Fruit Yield Under Orchard Conditions. Scientia Horticulture 4: 371-377.
• Björkman T, Blanchard LM, Harman GE (1998) Growth Enhancement of shrunken-2 (sh2) Sweet Corn by Trichoderma harzianum 1295-22: Effect of Environmental Stress. Journal of the American Society for Horticultural Sciences 123(1):35-40.
• Bora T (2002) Bitki Hastalıklarıyla Biyolojik Savaşta Gelişmeler ve Türkiye’ de Durum. Türkiye 5. Biyolojik Mücadele Kongresi. 4-7 Eylül 2002. Erzurum. 14s.
• Chen J (2006) The Combined of Chemical and Organic Fertilizers and/or Biofertilizer for Crop Growth and Salt Fertility. International Workshop on Sustained Management of the Soil-Rhizosphere System for Efficient Crop Production and Fertilizer Use. (Bangkok) 10900: 16-20.
• Di Marco S, Osti F (2007) Applications of Trichoderma to prevent Phaeomoniella Chlamydospora Infections in Organic Nurseries. Phytopathologia Mediterranea 46 (1) 73-83.
• Esitken A, Karlidag H, Ercisli S, Turan M, Sahin F (2003) The Effect of Spraying a Growth Promoting Bacterium on the Yield, Growth and Nutrient Element Composition of Leaves of Apricot (Prunus armeniaca L. cv. Hacihaliloglu). Australian Journal of Agricultural Research 54: 377-380.
• Ghabrial SA, Suzuki N (2009) Viruses of Plant Pathogenic Fungi. Annual Review of Phytopathology 47(1): 353-384.
• Larkin RP (2004) Development of Integrated Biological and Cultural Approaches for Control of Powdery Scab and Other Soil Borne Disease. USDA, ARS, New England Plant, Soil, and Water Lab Univ. of Maine, Orone, ME O 44469.
• Mahmood MAS (2014) Production of Bio-Formula of Bacillus subtilis in Oculaum for Control of Damping off Disease Caused by Pythium aphanidermatum on Cucumber Plants Under Green House Condition. Agriculture Science Plant Protection. Tikrit University.
• Moses RT (2006) Biological and Chemical Control of Fungi Seedling Diseases of Cowpea. M.Sc. Thesis. Department of Microbiology and Plant Pathology. Fac. of Natural and Agricultural Sciences. University of Pretoria. 67pp.
• Muhammad S, Amusa A (2003) In-vitro Inhibition of Growth of Some Seedling Blight Inducing Pathogens by Compost-Inhabiting Microbs. Journal of Biotechnology 2: 161-164.
• Mervat AA, Shawky SM, Shaker GS (2012) Comparative Efficacy of Some Bioagents, Plant Oil and Plant Aqueous Extracts in Controlling Meloidogyne incognita on Growth and Yield of Grapevines. Annals of Agricultural Science 57(1): 7-18.
• Özaktan H, Aysan Y, Yıldız F, Kınay P (2010) Fitopatolojide Biyolojik Mücadele. Türkiye Biyolojik Mücadele Dergisi 1(1): 61-78.
• Raudales RE, McSpadden Gardener BB (2008) Microbial Biopesticides for the Control of Plant Diseases in Organic Farming. Agriculture and Natural Resources Fact Sheet. HYG-3310-08. The Ohio State University Extension. 5p.
• Sabir A, Yazici MA, Kara Z, Sahin F (2012) Growth and Mineral Acquisition Response of Grapevine Rootstocks (Vitis spp.) to Inoculation with Different Strains of Plant Growth-Promoting Rhizobacteria (PGPR). Journal of Science Food and Agriculture 92(10): 2148-2153.
• Szczech M, Shoda M (2004) Biocontrol of Rhizoctonia Damping - off of Tomato by Bacillus subtilis Combined with Burkholderia Cepacia. Phytopathology 152: 549-556.
• Şahin F (2010) Organik Bitkisel Üretimde Biyoteknoloji ve Ar-Ge. Türkiye IV. Organik Tarım Sempozyumu, 28 Haziran - 1 Temmuz 2010. Erzurum (Çağrılı Bildiri).
• Thomas C (2009) Managing Plant Diseases With Biofungicides. Virginia Cooperative Extension Virginiatech. Virginia Polytechnic Institute and State University. 2906-1298. 3p.
• Yedidia I, Benhamou N, Kapulnik Y, Chet I (2000) Induction and Accumilation of PR Proteins Activity during Early Stages of Root Colonization by the Mycoparasite Trichoderma harzianum Strain T203. Plant Physiology and Biochemistry 38: 863-873.
• Yedidia I, Srivastva AK, Kapulnik Y, Chet I (2001) Effect of Trichoderma harzianum on Microelement Concentrations and Increased Growth of Cucumber Plants. Plant and Soil 235: 235-242.
• Yonsel Ş, Demir M (2011) Kiraz ve Elma Fidanları ve Domates Fidelerinde Trichoderma harzianum KUEN 1585 Uygulamaları. Çanakkale Tarımı Sempozyumu Dünü, Bugünü Geleceği, 10234-11 Ocak 2011 Bildiriler 297-301.
• Zhou T, Paulitz C (1993) In-vitro and In-vivo effects of Pseudomonas spp. on Pythium aphanidermatum: Zoospores Behavior in Exudates and on the Rhizoplane of Bacteria Treated Cucumber Roots. Phytopathology 84(8): 872-876.