Zencefilin (Zingiber officinale Roscoe) Diazinon Toksisitesine Karşı Koruyucu Rolünün Allium cepa L. Meristem Hücrelerinde Araştırılması

Yıl 2021, Cilt: 18 Sayı: 1, 65 - 72, 30.06.2021

Kültiğin Çavuşoğlu , Oksal Macar , Tuğçe Kalefetoğlu Macar , Emine Yalçın

https://doi.org/10.25308/aduziraat.814545

Öz

Yaygın olarak kullanılan bir pestisit olan diazinon hedef olmayan canlılar üzerinde toksik etkilere sahiptir. Bu çalışma, diazinonun meristem hücrelerinde teşvik ettiği toksisiteye karşı zencefilin koruyucu ve iyileştirici etkisini fizyolojik, genotoksik ve biyokimyasal parametrelerle araştırmayı hedeflemiştir. Ayrıca kök meristem hücrelerinde meydana gelen hasarlar incelenmiştir. Bu amaçla A. cepa soğanları 6 gruba ayrıldı (kontrol, 240 mg/L zencefil, 480 mg/L zencefil, 500 mg/L diazinon, 500 mg/L diazinon + 240 mg/L zencefil ve 500 mg/L diazinon + 480 mg/L zencefil). Diazinon süperoksit dismutaz aktivitesi, katalaz aktivitesi, malondialdehit seviyesi, mikronükleus sıklığı, kromozomal anormallik yoğunluğu ve meristematik hücre hasarlarını arttırmış; ancak mitotik indeksi, çimlenme yüzdesi, kök uzunluğu, ağırlık artışı azaltmıştır. Zencefil, diazinon ile birlikte uygulandığında doza bağlı olarak tüm bu toksisite göstergelerini düşürmüştür. Bu çalışma, A. cepa modeli kullanılarak diazinonun hedef olmayan organizmalar üzerindeki toksik etkilerine karşı zencefilin koruyucu bir rolünü açıkça göstermiştir.

Anahtar Kelimeler

antioksidant, fonksiyonel gıda, genotoksisite, mikronükleus

Destekleyen Kurum

destek alınmamıştır

Investigation of the Protective Role of Ginger (Zingiber officinale Roscoe) Against Diazinone Toxicity in Allium cepa L. Meristem Cells

Öz

Diazinon, a widely used pesticide, has toxic effects on non-target organisms. This study aimed to investigate the protective and healing effect of ginger against the toxicity promoted by diazinon in meristem cells with physiological, genotoxic and biochemical parameters. In addition, root meristem cell damages were examined. For this purpose, A. cepa bulbs were divided into 6 groups (control, 240 mg/L ginger, 480 mg/L ginger, 500 mg/L diazinone, 500 mg/L diazinone + 240 mg/L ginger and 500 mg/L diazinone + 480 mg/L ginger). Diazinon increased superoxide dismutase activity, catalase activity, malondialdehyde level, micronucleus frequency, chromosomal abnormality density and meristematic cell damage; but reduced the mitotic index, germination percentage, root length, weight gain. Ginger reduced all these toxicity indicators depending on the dose when administered with diazinon. This study clearly demonstrated the protective role of ginger against the toxic effects of diazinon on non-target organisms using the A. cepa model.

Anahtar Kelimeler

antioxidant, functional food, genotoxicity, micronucleus

Kaynakça

• Abdel-Daim MM, Abushouk AI, Alkhalf MI, Toraih EA, Fawzy MS, Ijaz H, Aleya L, Bungau SG (2018) Antagonistic Effects of Spirulina platensis on Diazinon-Induced Hemato-biochemical Alterations and Oxidative Stress in Rats. Environmental Science and Pollution Research 25(27): 27463-27470.
• Abdelkhalek NK, Eissa IA, Ahmed E, Kilany OE, El-Adl M, Dawood MA, Hassan AM, Abdel-Daim MM (2017) Protective Role of Dietary Spirulina platensis against Diazinon-Induced Oxidative Damage in Nile tilapia; Oreochromis niloticus. Environmental Toxicology and Pharmacology 54: 99-104.
• Al Hroob AM, Abukhalil MH, Alghonmeen RD, Mahmoud AM (2018) Ginger Alleviates Hyperglycemia-Induced Oxidative Stress, Inflammation and Apoptosis and Protects Rats against Diabetic Nephropathy. Biomedicine and Pharmacotherapy 106: 381-389.
• Almostady AA, Mutwakil MH, ElAssouli MZ, Ahmed MM, El Assouli SM (2015) Genotoxicity and Antigenotoxicity Activites of Rhazya stricta and Zingiber officinale Single and in Combination. American-Eurasian Journal of Agricultural and Environmental Science 15: 1392-1401.
• Atik M, Karagüzel O, Ersoy S (2007) Sıcaklığın Dalbergia sissoo tohumlarının Çimlenme Özelliklerine Etkisi. Mediterranean Agricultural Sciences 20(2): 203-210.
• Beauchamp C, Fridovich I (1971) Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels. Analytical Biochemistry 44: 276-287.
• Beers RF, Sizer IW (1952) Colorimetric Method for Estimation of Catalase. Journal of Biological Chemistry 195: 133-139.
• Bıçakçı U, Çavuşoğlu K, Yapar K, Acar A, Yalçın E (2017) Allium cepa L. Kök Ucu Hücrelerinde Diazinon Toksisitesinin Araştırılması. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi 7(3): 49-56.
• Davies DB, Holub BJ (1980) Toxicological Evaluation of Dietary Diazinon in The Rat. Archives of Environmental Contamination and Toxicology 9: 637-650.
• Dehghani MH, Kamalian S, Shayeghi M, Yousefi M, Heidarinejad Z, Agarwal S, Gupta VK (2019) High-performance Removal of Diazinon Pesticide from Water Using Multi-walled Carbon Nanotubes. Microchemical Journal 145:486-491.
• El-Shenawy NS, El-Salmy F, Al-Eisa RA, El-Ahmary B (2010) Amelioratory Effect of Vitamin E on Organophosphorus Insecticide Diazinon-Induced Oxidative Stress in Mice Liver. Pesticide Biochemistry and Physiology 96(2): 101-107.
• Fenech M (1993) The Cytokinesis-Block Micronucleus Technique: A Detailed Description of the Method and Its Application to Genotoxicity Studies in Human Populations. Mutation Research 285: 35-44.
• Fenech M, Chang WP, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E (2003) HUMN Project: Detailed Description of the Scoring Criteria for the Cytokinesis-Block Micronucleus Assay Using Isolated Human Lymphocyte Cultures. Mutation Research - Genetic Toxicology and Environmental Mutagenesis 534: 65-75.
• Fioresi VS, de Cássia Ribeiro Vieira B, de Campos JMS, da Silva Souza T (2020) Cytogenotoxic Activity of The Testicides Imidacloprid and Iprodione on Allium cepa Root Meristem. Environmental Science and Pollution Research 1-11.
• Galloway T, Handy R (2003) Immunotoxicity of organophosphorous pesticides. Ecotoxicology 12: 345-363 Hajirezaee S, Mirvaghefi AR, Farahmand H, Agh N (2016) Effects of Diazinon on Adaptation to Sea-water by The Endangered Persian Sturgeon, Acipenser persicus, Fingerlings. Ecotoxicology and Environmental Safety 133: 413-423.
• Han YA, Song CW, Koh WS, Yon GH, Kim YS, Ryu SY, Kwon HJ, Lee KH (2013) Anti‐inflammatory Effects of The Zingiber officinale Roscoe Constituent 12‐Dehydrogingerdione in Lipopolysaccharide‐stimulated Raw 264.7 Cells. Phytotherapy Research 27(8): 1200-1205.
• Hao M, Liu R (2019) Molecular Mechanism of CAT and SOD Activity Change under MPA-CdTe Quantum Dots Induced Oxidative Stress in the Mouse Primary Hepatocytes. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 220: 117104: 1-11
• Kalefetoğlu Macar T, Macar O, Yalçın E, Çavuşoğlu K (2020) Resveratrol Ameliorates the Physiological, Biochemical, Cytogenetic, and Anatomical Toxicities Induced by Copper (II) Chloride Exposure in Allium cepa L. Environmental Science and Pollution Research 27(1): 657-667.
• Karamian A, Shokrzadeh M, Ahmadi A (2016) The Potential Chemoprotective Effects of Melatonin against Genotoxicity Induced by Diazinon in Human Peripheral Blood Lymphocytes. Toxicology and Industrial Health 32(2): 360-366.
• Khanna N, Sharma S, (2013) Allium cepa Root Chromosomal Aberration Assay: A Review. Indian Journal of Pharmaceutical and Biological Research, 1(03): 105-119.
• Macar O, Macar TK, Çavuşoğlu K, Yalçın E (2020) Determination of Protective Effect of Carob (Ceratonia siliqua L.) Extract against Cobalt (II) Nitrate-Induced Toxicity. Environmental Science and Pollution Research 27: 40253-40261.
• Mao QQ, Xu XY, Cao SY, Gan RY, Corke H, Li HB (2019) Bioactive Compounds and Bioactivities of Ginger (Zingiber officinale Roscoe). Foods 8(6): 185.
• Pandir D (2018) Assesment of the Genotoxic Effect of the Diazinon on Root Cells of Allium cepa (L.). Brazilian Archives of Biology and Technology 61: e18160390.
• Saeidi M, Naeimi A, Komeili M (2016) Magnetite Nanoparticles Coated with Methoxypolyethylene Glycol as an Efficient Adsorbent of Diazinon Pesticide from Water. Advances in Environmental Technology 2(1): 25-31.
• Sh G, Shabestani Monfared A, Zabihi E, Khoshbin Khoshnazar A, Asadi J, Abedian Z, Borzoueisileh S (2020) Changes in the Radiation Toxicity of Human Lymphoblastic T-cell Line (Jurkat) by a Common Pesticide: Diazinon. Journal of Biomedical Physics and Engineering 10(2): 147-154.
• Shah MD, Iqbal M (2010) Diazinon-induced Oxidative Stress and Renal Dysfunction in Rats. Food and Chemical Toxicology 48(12): 3345-3353.
• Staykova TA, Ivanova EN, Velcheva IG (2005) Cytogenetic Effect of Heavy Metal and Cyanide in Contamined Waters from the Region of Southwest Bulgaria. Journal of Cell and Molecular Biology 4(1): 41-46.
• Stoner GD (2013) Ginger: is It Ready for Prime Time?. Cancer Prevention Research 6(4): 257-262.
• Tabasideh S, Maleki A, Shahmoradi B, Ghahremani E, McKay G (2017) Sonophotocatalytic Degradation of Diazinon in Aqueous Solution Using Iron-doped TiO2 Nanoparticles. Separation and Purification Technology 189: 186-192.
• Trimedona N, Rahzarni R, Syahrul S, Muchrida Y, Roza I (2020) Antioxidant Properties of Herbal Tea Prepared from Red Dragon Fruit Peel with The Addition of Ginger. Journal of Applied Agricultural Science and Technology 4(2): 181-188.
• Unyayar S, Celik A, Cekic FO, Gozel A (2006) Cadmium-Induced Genotoxicity, Cytotoxicity, and Lipid Peroxidation in Allium sativum and Vicia faba. Mutagenesis 21:77-81.
• USEPA (US Environmental Protection Agency) (1996) Seed Germination/Root Elongation Toxicity Test, OPPTS 850.4200, EPA712/C-96/154. In: Ecological Effects Test Guidelines. Washington, DC.
• WHO (World Health Organization) (1985) Guide to short-term tests for detectingmutagenic and carcinogenic chemicals. Environ. Health Criteria 51,Geneva, pp. 208
• Zargar A, Taheri Mirghaed A, Mirzargar SS, Ghelichpour M, Yousefi M, Hoseini SM (2020) Dietary Ginger Administration Attenuates Oxidative Stress and Immunosuppression Caused by Oxytetracycline in Rainbow Trout (Oncorhynchus mykiss). Aquaculture Research 51(10): 4215-4224.
• Zou J, Yue J, Jiang W, Liu D (2012) Effects of Cadmium Stress on Root Tip Cells and Some Physiological Indexes in Allium cepa var. agrogarum L. Acta Biologica Cracoviensia Series Botanica 54: 129-141.
• Zulaikhah ST (2018) Effects of Tender Coconut Water on Antioxidant Enzymatic Superoxida Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx) and Lipid Peroxidation in Mercury Exposure Workers. International Journal of Science and Research 4(12): 517-524.