The Effect on Antioxidant Defense System of Chondroitin-4-Sulphate in Human Erythrocytes Subject to High Glucose Levels: In The Laboratory Conditions

Yıl 2018, Cilt: 8 Sayı: 2, 98 - 114, 28.12.2018

Seda Balkan

Öz

The purpose of this research was to evaluation the effects on antioxidant defense system of chondroitin-4-sulphate (C4S) in high glucose treated human erythrocytes. 10-20 ml venous blood samples from ten healthy volunteers ages 20-40, nonsmoker, were accumulated in ethylenediamine tetraacetic acid (EDTA) vials for the preparation of red blood cell (RBC) suspensions.  Blood for in vitro treatments was divided into six groups. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), acetylcholinesterase (AChE), the levels of glutathione (GSH) and malondialdehyde (MDA) were calculated to determine the effects on antioxidant defense system in all groups. As a result of the statistical evaluations, the significant increases (p<0.001) in GSH-Px and AChE enzyme activities were observed in the groups in which C4S applied compared to the groups in which glucose alone applied. At the same time, significant decrease (p<0.001) in MDA levels was obtained in the groups in which C4S applied compared to the groups in which glucose alone applied. These acquired findings have shown that in the erythrocytes to which high amount of glucose has been treated; C4S may have a positive impact upon antioxidant defense systems. As the results of this study, C4S can be said to be benefited as an antioxidant.

Anahtar Kelimeler

Human erythrocytes, Antioxidant defense, Chondroitin-4-sulphate, Glucose, Antioxidant enzymes

Laboratuvar Koşullarında Yüksek Glukoza Maruz Kalmış İnsan Eritrositlerinde Kondroitin-4-Sülfatın Antioksidan Savunma Sistemi Üzerine Etkisi

Öz

Bu çalışmada, yüksek glukoza maruz kalmış insan eritrositlerinde  kondroitin-4-sülfatın (C4S) antioksidan savunma sistemine olan etkisi araştırılmıştır. Eritrosit süspansiyonu hazırlamak için, sigara içmeyen, 20-40 yaş aralığında sağlıklı gönüllülerden 10-20 ml venöz kanı etilendiamin tetraasetik asitli (EDTA) tüplere toplanmıştır. İn vitro uygulamalar için kanlar 6 gruba ayrılmıştır. Tüm gruplarda antioksidan savunma sistemi üzerine olan etkilerin belirlenmesi için süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSH-Px), asetilkolinesteraz (AChE) enzim aktiviteleri, glutatyon (GSH) ve MDA düzeyleri ölçülmüştür. İstatistiksel değerlendirmeler sonucunda, C4S uygulanan gruplar ile sadece glukoz uygulanan gruplar karşılaştırıldığında GSH-Px ve AChE enzim aktivitelerinde önemli ölçüde artış (p<0,001), MDA düzeylerinde de önemli ölçüde azalış olduğu (p<0,001) tespit edilmiştir. Elde edilen bu bulgular, yüksek glukoz içeren eritrositlerde C4S'nin uygulanabileceğini ve C4S'nin antioksidan savunma sistemleri üzerine olumlu etkileri olabileceğini göstermektedir. Sonuç olarak C4S'nin etkili bir antioksidan olduğu söylenebilir.

Anahtar Kelimeler

İnsan eritrositleri, Antioksidan savunma, Kondroitin-4-sülfat, Glukoz, Antioksidan enzimler

Kaynakça

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