Biochemical characterization of four different genotypes of Flax (Linum usitatissimum L.) seeds

Yıl 2017, Cilt: 1 Sayı: 1, 12 - 17, 25.10.2017

Gökhan Sadi , Burhan Karabakan Muhammed Aasım

https://doi.org/10.30616/ajb.342914

Öz

Flaxseed (Linum usitatissimum L.) is an oilseed
used in industrial and natural health products. It accumulates many
biologically active compounds having strong phytochemicals and antioxidant
properties. This study aims to provide an entire portfolio of bioactive
compounds present in four different genotypes of flaxseed (Avangard,
Bony Doon, Linda and Linton). Biochemical diversity among these four
different types of seed with respect to oxidant and antioxidant parameters
(phenolics, flavonoids, β-karoten, lycopen, DPPH scavenging activity, MDA,
proline, SOD, CAT, APx and GR) were investigated. According to results, Linda types are found to have
unusual phytochemicals and antioxidant profile since its oxidative status tends
to the production of oxidant molecules which was reflected in differences in
radical scavenging, MDA and antioxidant enzyme systems. Other
flaxseeds demonstrate noticeable activities with their antioxidant potential
together with their high phytochemicals.
Therefore, they may be utilized as a promising source of therapeutics
since they might provide an appropriate source of antioxidants.

Anahtar Kelimeler

Flaxseed, Phytochemicals, Bioactive compounds

Kaynakça

• Ábrahám E, Hourton-Cabassa C, Erdei L, Szabados L (2010) Methods for Determination of Proline in Plants. In: Methods Mol. Biol. pp 317–331.
• Aebi H (1984) In Methods in Enzymology. In: Catalase Vitr. Academy Press, NY, pp 121–126.
• Amawi H, Ashby C, Samuel T, Peraman R, Tiwari AK (2017) Polyphenolic Nutrients in Cancer Chemoprevention and Metastasis: Role of the Epithelial-to-Mesenchymal (EMT) Pathway. Nutrients 9(8):911.
• Czemplik M, Kulma A, Bazela K, Szopa J (2012) The biomedical potential of genetically modified flax seeds overexpressing the glucosyltransferase gene. BMC Complement Altern Med 12:251.
• Foster JG, Hess JL (1980) Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen. Plant Physiol 66:482–487.
• Goyal A, Sharma V, Upadhyay N, Gill S, Sihag M (2014) Flax and flaxseed oil: an ancient medicine & modern functional food. J Food Sci Technol 51:1633–1653.
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275.
• Maity P, Samanta S, Nandi AK, Sen IK, Paloi S, Acharya K, Islam SS (2014) Structure elucidation and antioxidant properties of a soluble β-d-glucan from mushroom Entoloma lividoalbum. Int J Biol Macromol 63:140–149.
• Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469–474.
• Pal J, Ganguly S, Tahsin KS, Acharya K (2010) In vitro free radical scavenging activity of wild edible mushroom, Pleurotus squarrosulus (Mont.) Singer. Indian J Exp Biol 48:1210–1218.
• Prasad K (2000) Flaxseed: a source of hypocholesterolemic and antiatherogenic agents. Drug News Perspect 13:99–104. Prasad K (2009) Flaxseed and Cardiovascular Health. J Cardiovasc Pharmacol 54:369–377.
• Sairam RK, Saxena DC (2000) Oxidative Stress and Antioxidants in Wheat Genotypes: Possible Mechanism of Water Stress Tolerance. J Agron Crop Sci 184:55–61.
• Savicka M, Skute N (2010) Effects of high temperature on malondialdehyde content, superoxide production and growth changes in wheat seedlings (Triticum aestivum L.). Ekologija 56:26–33.
• Taga MS, Miller EE, Pratt DE (1984) Chia seeds as a source of natural lipid antioxidants. J Am Oil Chem Soc 61:928–931.
• Turkoglu A, Duru ME, Mercan N, Kivrak I, Gezer K (2007) Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chem 101:267–273.
• Wang L, Chen J, Thompson LU (2005) The inhibitory effect of flaxseed on the growth and metastasisof estrogen receptor negative human breast cancer xenograftsis attributed to both its lignan and oil components. Int J Cancer 116:793–798.