Eisenia hortensis Sölomositlerinde Bentazone ve Chloridazon Herbisitlerinin Genotoksisitesinin Değerlendirilmesi
Öz
Bu çalışmada, Bentazone ve Chloridazon herbisitlerinin Eisenia hortensis türleri üzerindeki genotoksik etkileri araştırılmıştır. E. hortensis türleri Afyonkarahisar'daki doğal yaşam alanlarından toplanmıştır. Solucan sölomositlerinde genotoksisiteyi değerlendirmek için Comet ve Mikronükleus testi yapıldı. Bentazone herbisitlerin LD50 değeri Chloridazon için sırasıyla 236 ppm ve 76.6 ppm olarak kaydedildi. Daha sonra LD50/2, LD50 and 2XLD50 konsantrasyonlarında Bentazon ve Chloridazon herbisitleri E. hortensis'e 48 saat süreyle uygulandı. Her iki herbisitte de DNA'da konsantrasyona bağlı artış ve kromozomal hasar gözlendi (P <0.05). En yüksek DNA hasarı ve kromozomal sapmaları, diğer konsantrasyonlara ve kontrol grubuna kıyasla en yüksek dozlarda tespit edildi. Bentazone ve Chloridazon'un E. hortensis’ te DNA hasarı ve kromozomal sapmalara neden olduğu sonucuna varılmıştır.
Anahtar Kelimeler
Bentazon Chloridazon Eisenia hortensis Genotoksisite Comet yöntemi Mikronukleus
Destekleyen Kurum
Afyon Kocatepe Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi
Proje Numarası
15.FEN.BİL.44
Kaynakça
- Ahmadi F., Jamali N., Jahangard-Yekta S., Jafari S., Nouri S., Najafi F. and Rahimi-Nasrabadi M., 2011. The experimentaland theoretical QM/MM study of interaction of chloridazon herbicide with ds-DNA. Spectrochimica Acta Part A: Molecularand Biomolecular Spectroscopy, 1011, 1004-1012.
- Aydın H., 2006. Toprak solucanlarının Çevre Toksikolojisi Yönünden Değerlendirilmesi. İstanbul Üniversites Veterinerlik Fakültesi Dergisi, 32, 75-79
- Brkic D., Szakonyne-Pasics I., Gasic S., Teodorovic I., Raskovic B., Brkic N. and Neskovic N., 2015. Subacute and subchronic toxicity of Avalonregd mixture (bentazone+dicamba) to rats. Environmental Toxicology and Pharmacology, 13,1-30.
- Castellanos L.R. and Hernandez T.C.S., 2007. Earthworm biomarkers of pesticide contamination: current status and perspectives. Journal of Pesticide Science 32(4),360-371
- Charles B. K., Wıllıam W. B.., Seth R.K., Bonnıe U., and Thomas F. S., 1995. Stages of Embryonic Development of the Zebrafish. Developmental Dynamıcs,203, 253-310
- Ciğerci, İ., Ali, M.M., Yüksek, Ş., Liman, R., 2016. Genotoxicity assessment of cobalt chloride in Eisenia hortensis earthworms coelomocytes by comet assay and micronucleus test. Chemosphere, 755, 754-757.
- Collins A., Hartmann A., Agurell E., Beevers C., Brendler-Schwaab S., Burlinson B. and Clay P., 2003. Recommendations for conducting the in vivo alkaline Comet assay. Mutagenesis, 18(1), 45-51.
- Gambi N., Pasteris A., Fabbri E., 2007. Acetylcholinesterase activity in the earthworm (Eisenia andrei) at different conditions of carbaryl exposure. Comparative Biochemistry & Physiology, 145 (4), 678–685.
- Kaya B., Marcos R., Yanikoğlu A. and Creus A., 2004. Evaluation of the genotoxicity of four herbicides in the wing spot test of Drosophila melanogaster using two different strains. Mutation Research, 55, 53-62.
- Lanno R., Wells J., Conder J., Bradham K., Basta N., 2004. The bioavailability of chemicalsin soil for earthworms. Ecotoxicol. Environ. Saf. 57 (1), 39–47.
- Lina S., Yarong G.., Jiao N., Shanning Lou., Fujiang H. 2020. Herbicide Applications Increase Greenhouse Gas Emissions of Alfalfa Pasture in the Inland Arid Region of Northwest China. PeerJ. May 25,8, 9231.
- Lukkari T., Taavitsainen M., Soimasuo M., Oikari A., Haimi J., 2004. Biomarker responses of the earthworm (Aporrectodea tuberculata) to copper and zinc exposure: differences between populations with and without earlier metal exposure. Environ. Pollut, 129, 377–386.
- Reinecke S.A., Reinecke, A.J., 2004. The comet assay as biomarker of heavy metal genotoxicity in earthworms. Arch. Environ. Contam. Toxicol. 46, 208-215.
- Reinecke S.A. and Reinecke A.J., 2007. The impact of organophosphate pesticides in orchards on earthworms in the Western Cape, South Africa. Ecotoxicol. Environ. Saf. 66 (2), 244–251.
- Saglıo P., Olsen K.H., Bretaud S., 2001. Behavioral and olfactory responses to prochloraz, bentazone, and nicosulfuron contaminated flows in golfish. Arch Environ Contam Toxicol, 41(2), 192-200.
- Venkateswara Rao J., Kavitha P., Jakka N.M., Sridhar V., Usman P.K., 2007. Toxicity of organophosphates on morphology andocomotor behavior in brine shrimp (Artemia salina). Arch. Environ. Contam. Toxicol. 53, 227–232.
- Xiao H., Zhou Q.X., Liang J.D., 2004. Single and joint effects of acetochlor and urea on earthworm Eisenia foelide populations in phaiozem. Environmental Geochemistry and Health, 26, 277–283.
- Xiao N.W., Jing B.B., Ge F., Liu, X.H., 2006. The fate of herbicide acetochlor and its toxicity to Eisenia fetida under laboratory conditions. Chemosphere, 62, 1366– 1373.
- Zhan, Y., 2012. Effects of silver nanoparticles on bacteria and earthworms. Master's thesis , Lincoln University, ABD, 129.
Öz
In current study, genotoxic effects of Bentazone and Chloridazon herbicides on Eisenia hortensis species were investigated. The species of E. hortensis were collected from the natural habitats in Afyonkarahisar. Comet assay and Micronucleus test was conducted to evaluate the genotoxicity in earthworm coelomocytes. The LD50 value of Bentazone herbicides was noted as 236 ppm and 76.6 ppm for Chloridazon, respectively. Then LD50/2, LD50 and 2XLD50 concentrations of Bentazone and Chloridazon herbicides were applied to E. hortensis for 48 h. Concentration dependent increase in DNA and chromosomal damage was observed (P < 0.05) by both herbicides. Highest DNA damage and micronucleus formation were noticed at highest doses compared to other concentrations and control group. It was concluded that Bentazone and Chloridazon induced DNA damage and chromosomal aberrations failure in E. hortensis earthworms.
Anahtar Kelimeler
Bentazone Chloridazon Chloridazon Eisenia hortensis Eisenia hortensis Genotoxicity Comet assay Micronucleus
Proje Numarası
15.FEN.BİL.44
Kaynakça
- Ahmadi F., Jamali N., Jahangard-Yekta S., Jafari S., Nouri S., Najafi F. and Rahimi-Nasrabadi M., 2011. The experimentaland theoretical QM/MM study of interaction of chloridazon herbicide with ds-DNA. Spectrochimica Acta Part A: Molecularand Biomolecular Spectroscopy, 1011, 1004-1012.
- Aydın H., 2006. Toprak solucanlarının Çevre Toksikolojisi Yönünden Değerlendirilmesi. İstanbul Üniversites Veterinerlik Fakültesi Dergisi, 32, 75-79
- Brkic D., Szakonyne-Pasics I., Gasic S., Teodorovic I., Raskovic B., Brkic N. and Neskovic N., 2015. Subacute and subchronic toxicity of Avalonregd mixture (bentazone+dicamba) to rats. Environmental Toxicology and Pharmacology, 13,1-30.
- Castellanos L.R. and Hernandez T.C.S., 2007. Earthworm biomarkers of pesticide contamination: current status and perspectives. Journal of Pesticide Science 32(4),360-371
- Charles B. K., Wıllıam W. B.., Seth R.K., Bonnıe U., and Thomas F. S., 1995. Stages of Embryonic Development of the Zebrafish. Developmental Dynamıcs,203, 253-310
- Ciğerci, İ., Ali, M.M., Yüksek, Ş., Liman, R., 2016. Genotoxicity assessment of cobalt chloride in Eisenia hortensis earthworms coelomocytes by comet assay and micronucleus test. Chemosphere, 755, 754-757.
- Collins A., Hartmann A., Agurell E., Beevers C., Brendler-Schwaab S., Burlinson B. and Clay P., 2003. Recommendations for conducting the in vivo alkaline Comet assay. Mutagenesis, 18(1), 45-51.
- Gambi N., Pasteris A., Fabbri E., 2007. Acetylcholinesterase activity in the earthworm (Eisenia andrei) at different conditions of carbaryl exposure. Comparative Biochemistry & Physiology, 145 (4), 678–685.
- Kaya B., Marcos R., Yanikoğlu A. and Creus A., 2004. Evaluation of the genotoxicity of four herbicides in the wing spot test of Drosophila melanogaster using two different strains. Mutation Research, 55, 53-62.
- Lanno R., Wells J., Conder J., Bradham K., Basta N., 2004. The bioavailability of chemicalsin soil for earthworms. Ecotoxicol. Environ. Saf. 57 (1), 39–47.
- Lina S., Yarong G.., Jiao N., Shanning Lou., Fujiang H. 2020. Herbicide Applications Increase Greenhouse Gas Emissions of Alfalfa Pasture in the Inland Arid Region of Northwest China. PeerJ. May 25,8, 9231.
- Lukkari T., Taavitsainen M., Soimasuo M., Oikari A., Haimi J., 2004. Biomarker responses of the earthworm (Aporrectodea tuberculata) to copper and zinc exposure: differences between populations with and without earlier metal exposure. Environ. Pollut, 129, 377–386.
- Reinecke S.A., Reinecke, A.J., 2004. The comet assay as biomarker of heavy metal genotoxicity in earthworms. Arch. Environ. Contam. Toxicol. 46, 208-215.
- Reinecke S.A. and Reinecke A.J., 2007. The impact of organophosphate pesticides in orchards on earthworms in the Western Cape, South Africa. Ecotoxicol. Environ. Saf. 66 (2), 244–251.
- Saglıo P., Olsen K.H., Bretaud S., 2001. Behavioral and olfactory responses to prochloraz, bentazone, and nicosulfuron contaminated flows in golfish. Arch Environ Contam Toxicol, 41(2), 192-200.
- Venkateswara Rao J., Kavitha P., Jakka N.M., Sridhar V., Usman P.K., 2007. Toxicity of organophosphates on morphology andocomotor behavior in brine shrimp (Artemia salina). Arch. Environ. Contam. Toxicol. 53, 227–232.
- Xiao H., Zhou Q.X., Liang J.D., 2004. Single and joint effects of acetochlor and urea on earthworm Eisenia foelide populations in phaiozem. Environmental Geochemistry and Health, 26, 277–283.
- Xiao N.W., Jing B.B., Ge F., Liu, X.H., 2006. The fate of herbicide acetochlor and its toxicity to Eisenia fetida under laboratory conditions. Chemosphere, 62, 1366– 1373.
- Zhan, Y., 2012. Effects of silver nanoparticles on bacteria and earthworms. Master's thesis , Lincoln University, ABD, 129.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Mühendislik |
| Bölüm | Makaleler |
| Yazarlar | |
| Proje Numarası | 15.FEN.BİL.44 |
| Yayımlanma Tarihi | 31 Aralık 2020 |
| Gönderilme Tarihi | 17 Kasım 2020 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 20 Sayı: 6 |
