KUDRET NARI YAĞI YÜKLÜ BİYOBOZUNUR NANOKAPSÜLLERİN YARA ÖRTÜSÜ AMAÇLI DİZAYNI VE ÜRETİMİ

Yıl 2023, Cilt: 1 Sayı: 1, 15 - 22, 27.12.2023

Elif Bozkır , Fatma İnan , İrem Mukaddes Bilgiseven , Serdar Karakurt

Öz

Yara, kimyasal, mekanik veya termal hasar sonucu cilt yüzeyindeki doku bütünlüğünün bozulmasıyla meydana gelmektedir ve her an vücudumuzda komplikasyon yaratabilir. Yaranın hızlı bir şekilde tedavi edilebilmesi ve yaraların bakımı pansuman materyaline bağlı olarak değişmektedir. Elektro-eğirme yöntemi ile üretilen nanofiber yara örtüleri, yaraların hızlı iyileşmesi açısından umut verici bir yöntem haline gelmiştir. Nanofiber yara örtülerinde yer alan nano boyutlu lifler, hücre dışı matrikse benzerliklerinden dolayı ve ilaç/biyolojik ajan içerebileceğinden kaynaklı olarak yaraların iyileşmesi için uygun ortam sağladıkları için oldukça tercih edilen ideal bir tedavi yöntemidir. Bu çalışmada, aktif biyolojik ajanlar içeren Momordica charantia (kudret narı) yağı sayesinde yaraların iyileşmesini önemli ölçüde hızlandıran ve hasta refahını artıran polimer tabanlı nanofiber yara örtüsü geliştirilmesi amaçlanmıştır. Bunun için, Momordica charantia yağı polilaktikoglikolik asit içerisine enkapsüle edilerek, polietilen glikol tabanlı nanofiber yara örtüsü elektro-spreylenmiştir. Elde edilen nanofiber yara örtüsünün kimyasal yapısı ve morfolojisi için Fourier Dönüşümlü Kızılötesi (FTIR) ve Taramalı Elektron Mikroskobu (SEM) analizleri gerçekleştirilmiştir. Elde edilen nanofiber yara örtüsünün FTIR analizinde biyobozunur nanokapsüllerle geliştirilen nanofiber yara örtüsünün kimyasal bağları ayrıntılı bir şekilde gözlenmiştir. SEM analizinde ise eş boyutlu PLGA nanokapsüllerin elde edildiği ve kudret narı yağı ile kapsüllenen PLGA nanopartiküllerinin PEG nanosprey yapısı arasında homojen dağıldığı görülmüştür. Sonuç olarak yarayı enfeksiyonlardan korumak, iyileşme sürecini hızlandırmak amacı ile geliştirilen nanofiber yara örtüsü geleneksel aromatik bitkisel yağ tedavisi ile birleştirilerek entegre polimerik yara örtü malzemesi olarak kullanılabilecek önemli bir adaydır.

Anahtar Kelimeler

Elektrospinning, Momordica charantia, kudret narı, Nanofiber Yara Örtüsü, PEG, PLGA

Destekleyen Kurum

Selçuk Üniversitesi BAP Koordinatörlüğü

Proje Numarası

23402001

Teşekkür

Selçuk Üniversitesi Bilimsel Araştırma Projeleri Birimine [Proje no:23402001] destekleri için teşekkür ederiz.

DESIGN AND PRODUCTION OF MOMORDICA CHARANTIA OIL LOADED BIODEGRADABLE NANOCAPSULES FOR WOUND HEALING PURPOSES

Öz

Wound formation occurs as a result of chemical, chemical, mechanical, or thermal damage, leading to the disruption of tissue integrity on the skin surface, and can potentially create complications in our body at any moment. The rapid treatment of wounds and the care of wounds depend on the wound dressing material. Nanofiber wound dressings produced by the electrospinning method have become a promising method for the rapid healing of wounds. The nano-sized fibers in nanofiber wound dressings are highly preferred as an ideal treatment method due to their similarities to the extracellular matrix and their ability to contain drugs/biological agents, providing a suitable environment for wound healing. In this study, the aim was to develop a polymer-based nanofiber wound dressing that significantly accelerates the healing of wounds and enhances patient well-being, utilizing Momordica charantia (bitter melon) oil containing active biological agents. For this purpose, Momordica charantia oil was encapsulated into poly(lactic-co-glycolic acid) and electrospun into a polyethylene glycol-based nanofiber wound dressing. Fourier Transform Infrared (FTIR) and Scanning Electron Microscope (SEM) analyses were performed to determine the chemical structure and morphology of the obtained nanofiber wound dressing. In the FTIR analysis of the nanofiber wound dressing developed with biodegradable nanocapsules, the chemical bonds of the nanofiber wound dressing were observed in detail. In the SEM analysis, uniformly sized PLGA nanocapsules were obtained, and it was observed that PLGA nanoparticles encapsulated with bitter melon oil were homogeneously distributed among the PEG nanospray structure. In conclusion, the nanofiber wound dressing developed to protect wounds from infections and expedite the healing process is an important candidate as an integrated polymeric wound dressing material, combined with traditional aromatic herbal oil treatment.

Anahtar Kelimeler

Electrospinning, Momordica charantia, bitter melon, Nanofiber Wound Dressing, PEG, PLGA

Proje Numarası

23402001

Kaynakça

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