Effects of chronic administration of thymoquinone on penicillin induced epileptiform activity in rats

Yıl 2023, Cilt: 28 Sayı: 1, 38 - 47, 20.01.2023

Ersin Beyazçiçek , Duru Aslıhan Avci , Özge Beyazçiçek

https://doi.org/10.21673/anadoluklin.1168238

Öz

Aim: Thymoquinone (TQ) is derived from Nigella Sativa (NS) which is a traditional medicinal plant used as a spice, and medication in traditional medicine. This study aims to investigate the chronic effects of TQ, which has been shown anticancer, antioxidant, and neuroprotective effects, on experimental penicillin-induced epilepsy models in rats.

Methods: Forty-nine adult male Wistar rats were used in this study. The rats were divided into 7 groups as; sham (S), control (penicillin, CONT), diazepam (DZM), 10 mg/kg TQ (TQ10), 50 mg/kg TQ (TQ50), 10 mg/kg TQ+DZM (TQ10+DZM) and 50 mg/kg TQ+DZM (TQ50+DZM). TQ was administered intraperitoneally for 21 days. Epileptiform activity (EA) was initiated by intracortical administration of penicillin. Electrocorticographic (ECoG) data obtained from the recordings were analyzed. The latency, frequency, and amplitude of EA waves were analyzed statistically.

Results: There was no EA in the sham group. However, it was observed that the first spike-wave latency increased significantly in TQ10 and TQ50 groups compared to the CONT group. Except for some time periods, there was no significant difference between the groups according to spike-wave frequency, and spike-wave amplitude.

Conclusion: It was observed that the application of chronic thymoquinone on the experimental penicillin-induced EA model in rats did not affect the frequency and amplitude of spike-wave representing epileptic seizures. However, TQ was found to prolong the latency of the first EA.

Anahtar Kelimeler

Electrocorticography, epilepsy;, nigella sativa, rat;, thymoquinone, timokinon

Sıçanlarda kronik uygulanan timokinonun penisilin ile oluşturulmuş epileptiform aktivite üzerine etkileri

Öz

Amaç: Timokinon (TQ), geleneksel bir tıbbi bitki olan ve geleneksel tıpta baharat ve ilaç olarak kullanılan Nigella sativa (NS)’den elde edilmiştir. Bu çalışmanın amacı, antikanser, antioksidan ve nöroprotektif etkileri bilinen TQ’nun sıçanlarda deneysel penisilin kaynaklı epilepsi modelinde kronik etkilerini araştırmaktır.

Yöntemler: Bu çalışmada kırk dokuz adet erişkin erkek Wistar sıçan kullanıldı. Sıçanlar sham (S), kontrol (penisilin, CONT), diazepam (DZM), 10 mg/kg TQ (TQ10), 50 mg/kg TQ (TQ50), 10 mg/kg TQ+DZM (TQ10+DZM) ve 50 mg/kg TQ+DZM (TQ50+DZM) olmak üzere 7 gruba ayrıldı. TQ, 21 gün süresince intraperitoneal olarak uygulandı. Penisilin intrakortikal uygulanması ile epileptiform aktivite (EA) oluşturuldu. Kayıtlardan elde edilen elektrokortikografik veriler analiz edildi. EA dalgalarının latansı, frekansı ve amplitüdü istatistiksel olarak analiz edildi.

Bulgular: Sham grubunda EA gözlemlenmedi. Ancak TQ10 ve TQ50 gruplarının ilk diken-dalga latansının CONT grubuna göre anlamlı olarak arttığı gözlendi. Diken-dalga frekansı ve diken-dalga amplitüdü bakımından bazı zaman periyotları dışında gruplar arasında istatistiksel olarak anlamlı fark yoktu.

Sonuç: Sıçanlarda deneysel penisilinle oluşturulmuş EA modelinde kronik TQ uygulamasının, epileptik nöbetleri temsil eden herhangi bir diken dalga frekansını ve amplitüdünü etkilemediği gözlendi. Ancak, TQ›nun ilk EA başlama süresini uzattığı belirlendi.

Anahtar Kelimeler

elektrokortikografi;, epilepsi;, nigella sativa, sıçan, timokinon

Kaynakça

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